Thermo-Electric Properties of Quantum Point Contacts

I. Introduction II. Theoretical background (Landauer-Buttiker formalism of thermo-electricity, Quantum point contacts as ideal electron waveguides, Saddle-shaped potential) III. Experiments (Thermopower, Thermal conductance, Peltier effect) IV. ConclusionsComment: #4 of a series of 4 legacy reviews on QPC'

DNA Adducts of Decarbamoyl Mitomycin C Efficiently Kill Cells without Wild-Type p53 Resulting from Proteasome-Mediated Degradation of Checkpoint Protein 1

The mitomycin derivative 10-decarbamoyl mitomycin C (DMC) more rapidly activates a p53independent cell death pathway than mitomycin C (MC). We recently documented that an increased proportion of mitosene1-β-adduct formation occurs in human cells treated with DMC in comparison to those treated with MC. Here, we compare the cellular and molecular response of human cancer cells treated with MC and DMC. We find the increase in mitosene 1-β-adduct formation correlates with a condensed nuclear morphology and increased cytotoxicity in human cancer cells with or without p53. DMC caused more DNA damage than MC in the nuclear and mitochondrial genomes. Checkpoint 1 protein (Chk1) was depleted following DMC, and the depletion of Chk1 by DMC was achieved through the ubiquitin proteasome pathway since chemical inhibition of the proteasome protected against Chk1 depletion. Gene silencing of Chk1 by siRNA increased the cytotoxicity of MC. DMC treatment caused a decrease in the level of total ubiquitinated proteins without increasing proteasome activity, suggesting that DMC mediated DNA adducts facilitate signal transduction to a pathway targeting cellular proteins for proteolysis. Thus, the mitosene-1-β stereoisomeric DNA adducts produced by the DMC signal for a p53-independent mode of cell death correlated with reduced nuclear size, persistent DNA damage, increased ubiquitin proteolysis and reduced Chk1 protein

A multi-scale approach interpreting sediment processes and distribution from desert sand colour in central Saudi Arabia

In central Saudi Arabia the redness of sands observed on satellite imagery, often related to iron oxide amount, was used to better interpret geomorphic processes operating in the area. To compare variations between dune and interdune morphology, linear and dome dunes, and an ephemeral river partly buried under the sand sea, Nafud Al-Thuwayrat, a multiscale approach was used to identify the spatial variation in causes of iron oxide amounts and colour. Macro-scale mapping of Image Spectral Redness identified that the extent and intensity of colour grading across the orientation of well-defined linear and dome dune types, interdunes and the Wadi Al-Rimah, indicated sediment mixing of different provenance. Meso-scale chemical, physical and colour analysis of surface samples indicated that iron oxide coated grains were present within all the samples and was not the sole control on Image Spectral Redness. The reddest sediments were the best sorted medium-to fine-quartz sands with iron oxide bearing chemistry and the paler sediments contained coarser sands with additional feldspars, calcite and gypsum. Micro-scale analysis of grain characteristics found dune-interdune contrasts and that the reddest sands with iron coatings were of aeolian origin and the paler sands contained larger fluvial material mixed with reworked aeolian deposits. Dust in the area consists of high amounts of iron oxide and appears to be a major contributor to redness in the sand dunes. These controls on colour show the sand seas are paler westwards because a major river, and local drainage systems, deliver fluvial sediments from the carbonate and sandstone bedrock into the quartz rich aeolian material from the linear dunes. Evaporite deposits dominate in the paler interdunes, and south of the Wadi the dome dunes are the reddest as they are not migrating but building up. This multiscale approach has provided a modern analogy of processes for palaeoenvironmental studies

Evidence-based Kernels: Fundamental Units of Behavioral Influence

This paper describes evidence-based kernels, fundamental units of behavioral influence that appear to underlie effective prevention and treatment for children, adults, and families. A kernel is a behavior–influence procedure shown through experimental analysis to affect a specific behavior and that is indivisible in the sense that removing any of its components would render it inert. Existing evidence shows that a variety of kernels can influence behavior in context, and some evidence suggests that frequent use or sufficient use of some kernels may produce longer lasting behavioral shifts. The analysis of kernels could contribute to an empirically based theory of behavioral influence, augment existing prevention or treatment efforts, facilitate the dissemination of effective prevention and treatment practices, clarify the active ingredients in existing interventions, and contribute to efficiently developing interventions that are more effective. Kernels involve one or more of the following mechanisms of behavior influence: reinforcement, altering antecedents, changing verbal relational responding, or changing physiological states directly. The paper describes 52 of these kernels, and details practical, theoretical, and research implications, including calling for a national database of kernels that influence human behavior

A Systematic Review of the Pulmonary Microbiome in Patients with Acute Exacerbation COPD Requiring ICU Admission

Background: Chronic obstructive pulmonary disease (COPD) is a major health concern. Acute exacerbations (AECOPD) may require intensive care unit (ICU) admission and mechanical ventilation. Acute infections and chronic colonization of the respiratory system are known to precipitate AECOPD. Detailed knowledge of the respiratory microbiome could lead to effective treatment and prevention of exacerbations.Objective: The aim of this review is to summarize the available evidence on the respiratory microbiome of patients with a severe AECOPD requiring mechanical ventilation and intensive care admission. Methods: A systematic literature search was conducted to identify the published papers until January 2023. The collected data were then subjected to qualitative analysis. After the first analysis, a secondary focused review of the most recent publications studying the relationship between microbiome and mortality in AECOPD was performed. Results: Out of 120 screened articles six articles were included in this review. Potentially pathogenic microorganisms (PPMs) were identified in 30% to 72% of the patients with community-acquired bacteria, gram-negative enteric bacilli, Stenotrophomonas and Pseudomonas being the most frequently isolated. During hospitalization, 21% of patients experienced colonization by PPMs. Adequate antimicrobial therapy resulted in the eradication of 77% of the identified PPMs. However, 24% of the bacteria displayed multi-drug resistance leading to prolonged or failure of eradication. Conclusion: PPMs are prevalent in a significant proportion of patients experiencing an AECOPD. The most identified PPMs include community-acquired pathogens and gram-negative enteric bacilli. Notably, no differences in mortality or duration of ventilation were observed between patients with and without isolated PPMs. However, the included studies did not investigate the virome of the patients, which may influence the microbiome and the outcome of infection. Therefore, further research is essential to comprehensively investigate the complete microbial and viral composition of the lower respiratory system in COPD patients admitted to the ICU.</p