Life in Hampton Roads Report: The Tenth Annual Life in Hampton Roads Survey

[From the Executive Summary] The Social Science Research Center (SSRC) at Old Dominion University recently completed data collection for the tenth annual Life in Hampton Roads (LIHR) telephone survey. The purpose of the survey was to gain insight into residents’ perceptions of the quality of life in Hampton Roads. As in previous years, the project also investigated attitudes and perceptions of citizens regarding topics of local interest such as transportation and traffic, perceptions of police, health, community, education, work, experiences with flooding, and other issues. These are presented independently or as trending with previous years when appropriate. This year, the SSRC also partnered with the city of Chesapeake who provided additional questions for the survey. The SSRC completed interviews with 882 Hampton Roads residents via landline and cell phones

Author Correction: A cell-free platform for the prenylation of natural products and application to cannabinoid production

In the original version of this Article, the genotype of the M30 mutant presented in Fig. 3b was given incorrectly as Y288V/A232S, and the M31 mutant was given incorrectly as M1/A232S. The correct genotype of the M30 mutant is Y288A/A232S and for M31 it is Y288V/A232S. In addition, to keep consistency in genotype formatting, the genotype of the M27 mutant should be Y288V/G286S. The errors have been corrected in both the PDF and HTML versions of the Article

Life in Hampton Roads Report: The 12th Annual Life in Hampton Roads Survey

[From the Executive Summary] The Social Science Research Center (SSRC) at Old Dominion University is pleased to present the results from the 12th annual Life in Hampton Roads (LIHR) survey. The purpose of the survey was to gain insight into residents’ perceptions of the quality of life in Hampton Roads. It is important to note that the methodology for this year’s survey differs from previous Life in Hampton Roads surveys. The first ten years of the survey were conducted using a random sample of Hampton Roads residents via telephone. Last year state and university COVID-19 restrictions did not allow for staffing of the SSRC call center during the survey period. Therefore, on-line survey panels were used to solicit respondents to complete a web-based survey. This year, a mixed methods approach of telephone calls and web surveys were used to administer the survey. Given the continued and evolving pandemic conditions in Hampton Roads and the rest of the world, many of this year’s questions focused on residents’ experiences with and responses to continuing COVID-19 conditions

Life in Hampton Roads Report: The 11th Annual Life in Hampton Roads Survey

[From the Executive Summary] The Social Science Research Center (SSRC) at Old Dominion University is pleased to present the results from the 11th annual Life in Hampton Roads (LIHR) survey. The purpose of the survey was to gain insight into residents’ perceptions of the quality of life in Hampton Roads. It is important to note that the methodology for this year’s survey differs from previous Life in Hampton Roads surveys. The first ten years of the survey were conducted using a random sample of Hampton Roads residents via telephone. However, this year state and university COVID-19 restrictions did not allow for staffing of the SSRC call center during the survey period. Therefore, on-line survey panels were used to solicit respondents to complete a web-based survey. Given the pandemic conditions in Hampton Roads and the rest of the world, many of this year’s questions focused on residents’ experiences with and responses to COVID-19 conditions

The Evolution of Reduced Microbial Killing

Bacteria engage in a never-ending arms race in which they compete for limited resources and niche space. The outcome of this intense interaction is the evolution of a powerful arsenal of biological weapons. Perhaps the most studied of these are colicins, plasmid-based toxins produced by and active against Escherichia coli. The present study was designed to explore the molecular responses of a colicin-producing strain during serial transfer evolution. What evolutionary changes occur when colicins are produced with no target present? Can killing ability be maintained in the absence of a target? To address these, and other, questions, colicinogenic strains and a noncolicinogenic ancestor were evolved for 253 generations. Samples were taken throughout the experiment and tested for killing ability. By the 38th transfer, a decreased killing ability and an increase in fitness were observed in the colicin-producing strains. Surprisingly, DNA sequence determination of the colicin plasmids revealed no changes in plasmid sequences. However, a set of chromosomally encoded loci experienced changes in gene expression that were positively associated with the reduction in killing. The most significant expression changes were observed in DNA repair genes (which were downregulated in the evolved strains), Mg ion uptake genes (which were upregulated), and late prophage genes (which were upregulated). These results indicate a fine-tuned response to the evolutionary pressures of colicin production, with far more genes involved than had been anticipated

You Are The Right One

Eric Rhymes is dead. He was found at the old Jackson farm, a place many warn is haunted. In the town of Hemlock, Michigan, though, ghosts are all too common. The town is full of them, full of a history that details the slow decline of a region. It is a graveyard of crumbling factories, empty storefronts, foreclosed properties, and lonely train tracks. For every street, every home, every farm, there is said to be a story, and Daniel Meyers believes them all. In the wake of the latest tragedy, Dan begins looking at the history and scars of the community with a closer eye. But Dan’s own feelings for his older brother’s best friend, Sam, have become a haunting of their own. Embracing those feelings, Dan sets in motion a chain of events that will forever change him. You Are the Right One is the story of youthful yearnings, of nostalgic melancholy, and the inability to make peace with the past, the worst kind of haunting

Author Correction: A cell-free platform for the prenylation of natural products and application to cannabinoid production.

In the original version of this Article, the genotype of the M30 mutant presented in Fig. 3b was given incorrectly as Y288V/A232S, and the M31 mutant was given incorrectly as M1/A232S. The correct genotype of the M30 mutant is Y288A/A232S and for M31 it is Y288V/A232S. In addition, to keep consistency in genotype formatting, the genotype of the M27 mutant should be Y288V/G286S. The errors have been corrected in both the PDF and HTML versions of the Article

A cell-free platform for the prenylation of natural products and application to cannabinoid production

Producing individual cannabinoids by metabolically engineered microbes has proven challenging. Here, the authors develop a cell-free enzymatic prenylating system to generate isoprenyl pyrophosphate substrates directly from glucose and produce both common and rare cannabinoids at >1 g/L