Antimicrobial effect of Satureja cuneifolia Ten. essential oil

The essential oil composition of Satureja cuneifolia Ten., was analyzed with respect to samples picked in different vegetative stages in the region of Rotimije (Stolac, Herzegovina). We did not find oil of a composition characteristic of the Satureja genus. The researched species mostly did not contain either Thymol or Carvacrol. The antimicrobial effect of the oil was highly marked during the post - flowering period, especially for the Staphylococcus aureus bacterium. Essential oil fungicidal activity was best marked on Candida albicans yeast during the flowering period. Picking this plant at the proper time can considerably increase its therapeutic effect

Xeromorphism of trichomes in Lamiaceae species

The xeromorphic adaptation of epidermal cells of Lamiaceae plant leaves, the consequence of their adaptation to an arid habitat, was investigated. These xerophytic species inhibit transpiration in different ways, depending on the number, the form and the position of trichomes, whether they are dead hairs or live glandular structures. The Salvia genus is characterized on one hand by multicellular, uniseriate and scaled dead hairs and on the other hand there are several types of glandular structures, generally with voluminous heads. Lavender and rosemary (Lavandula angustifolia Mill, and Rosmarinus officinalis L.) have well-developed ramified forms of dead hairs with different ends and different levels of flexibility. The representatives of the Satureja genus have cone-shaped trichomes, showing predominant glandular structures (with unicellular base, unicellular stalk and twelve-cell head) and a slight structural variation. While glandular trichomes are frequent in this genus, non-glandular trichomes are rare and few. In Salvia, although no glandular trichomes are developed, it has numerous simple, uniseriate, multicellular hairs. This is how the leaf epidermis, by xeromorphic changes, ensures the preservation of the entire plant

Antimicrobial effect of Satureja cuneifolia Ten. essential oil

The essential oil composition of Satureja cuneifolia Ten., was analyzed with respect to samples picked in different vegetative stages in the region of Rotimije (Stolac, Herzegovina). We did not find oil of a composition characteristic of the Satureja genus. The researched species mostly did not contain either Thymol or Carvacrol. The antimicrobial effect of the oil was highly marked during the post - flowering period, especially for the Staphylococcus aureus bacterium. Essential oil fungicidal activity was best marked on Candida albicans yeast during the flowering period. Picking this plant at the proper time can considerably increase its therapeutic effect

Influence of Geographic Position, Leaf Surface and Genetic Variability on Content of Total Essential Oils in 12 Distinct Populations of Bay Laurel (Laurus nobilis L.)

Within research project “Taxonomy, Ecology and Utilization of Carob tree (Ceratonia siliqua L.) and Bay Laurel (Laurus nobilis L.) in Croatia” (HRZZ-IP-11-2013-3304), a total of 1200 plant samples were collected from 12 distinct populations of bay laurel in Croatian Adriatic area. After morphometric analyses of leaves, AFLP analyses of genetic variability, and variability of total essential oils content in leaves of bay laurel populations, significant correlation between leaf surface and essential oils content (Spearman’s Rank Order Corr. Coeff. rs = 0.15 ns) was not found, while the correlation between latitude and essential oils content was strongly negative (Spearman’s Rank Order Corr. Coeff. rs = - 0.78; p<0.05). However, the correlation between longitude and essential oils content was strongly positive (Spearman’s Rank Order Corr. Coeff. rs = 0.73; p<0.05). On the other hand, the results of Mantel test showed low, but positive and highly significant correlation between AFLP variability of populations and essential oils content (r = 0.39; p<0.01), while the significant correlation between AFLP variability of populations and leaf lamina surface (i.e. expectedly the most influential factor on accumulation of essential oils) was not obtained. Obtained results of these matrix correlations (i.e. Spearman’s Rank Order Correlations and Mantel test) correspond with the results of Friedman’s ANOVA and Kendall’s Coeff. of Concordance for variability of total essential oils content between the populations (ANOVA Chi Square = 21.88; p = 0.025 and Kendall’s Coeff. of Concordance = 0.99; Aver. rank r = 0.98). According to these results, it is possible to conclude that the populations of bay laurel from locations of south-east Croatian Adriatic islands and coastal area accumulate higher quantity of essential oils in the comparison with the populations of north-west islands and coastal area

Comparison of Morphological Characteristics of Carob Tree (Ceratonia siliqua L.) Pods and Seeds of Populations Collected from Two Distant Croatian Islands: Drvenik Mali and Mali Lošinj

It is well-known that carob tree (Ceratonia siliqua L.) is not overspread in Croatian north Adriatic islands and coast. It is hardly possible to find spontaneous (not planted) trees of Ceratonia siliqua northern from the island of Dugi Otok and Zadar coast. During the first investigated year of research project TEUCLIC financed by Croatian Foundation for Science, we found the northernmost habitat of carob tree on the island of Mali Lošinj. This paper deals with the results of morphometric analysis of carob tree pods and seeds of Mali Lošinj population, compared with the same analysis of carob tree population of Drvenik Mali, island placed in middle-Dalmatian archipelago, in order to find the possible differences of most important morphological traits between these two relatively distant populations. After provided morphometric research the differences in length and thickness of carob pods between these two populations were not significant. However, the pods of carob trees of Mali Lošinj population had significantly smaller weight and smaller number of seeds per pod in the comparison with Drvenik Mali population. Also, width of carob pods of Mali Lošinj population was significantly smaller in the comparison with Drvanik Mali population, as well as weight and average number of seeds per pod. The carob seeds of Drvenik Mali population are significantly longer, wider and heavier in the comparison with the Mali Lošinj population, in spite of the fact that the seeds of population of Mali Lošinj were significantly thicker in the comparison with seeds of Drvenik Mali population. The differences between these morphometric traits cannot be explained by environmental conditions. Differences in morphometric traits of carob pods and seeds between these two populations are primarily caused by genetic factors, which have to be confirmed by the analysis of genomic DNA isolated from the leaves

Kemijska analiza fenolnih spojeva vrsta roda Veronica

Identifikacija i kvantifikacija fenolnih spojeva različitih vrsta roda Veronica.LC-QTOF - tekućinska kromatografija sa spektrometrom mas

Kemijska analiza fenolnih spojeva vrsta roda Veronica

Identifikacija i kvantifikacija fenolnih spojeva različitih vrsta roda Veronica.LC-QTOF - tekućinska kromatografija sa spektrometrom mas

Antiphytoviral Activity of Satureja montana L. ssp. variegata (Host) P. W. Ball Essential Oil and Phenol Compounds on CMV and TMV

The essential oil of Satureja montana L. ssp. variegata (Host) P. W. Ball (Lamiaceae) was characterized by a high concentration of oxygenated monoterpenes (71.3%), among which carvacrol (19.4%) and thymol (16.6%) were the major compounds. When the essential oil was applied on local hosts Chenopodium amaranticolor Coste &amp; Reyn. and Chenopodium quinoa Willd. simultaneously with the infecting virus, the number of local lesions on both Tobacco mosaic virus (TMV) and Cucumber mosaic virus (CMV) infected plants was reduced for 29.2% and 24.1%, respectively. When applied individually for each virus, thymol was more effective in reducing CMV infection (33.2%), while carvacrol was more effective in reducing the TMV infection (34.3%). No synergistic effect of both monoterpenes was observed in the antiviral activity of the oil