Fast and semi-automatic S-wave and P-wave velocity estimations from landstreamer data: a field case from the Middle East

Seismic surface and body wave analyses are powerful tools for the geotechnical characterization of sites. The use of landstreamers facilitates the acquisition of dense data sets over large areas. However, efficient processing workflows are needed to estimate 3D velocity models from these massive data sets. For surface wave analysis, the manual picking of dispersion curves (DCs) of large data sets is very time-consuming, whereas the accuracy can be biased by operator choices. We apply a semi-automatic workflow for the analysis, processing, and interpretation of a large-scale landstreamer data set acquired for engineering purposes in the Middle East. The workflow involves the application of a validated automatic DC picking algorithm, and the transformation of the DCs into S- and P-wave velocity models through the wavelength-depth technique. The method has a high level of automation, is data driven and does not require extensive data inversion. Another remarkable benefit is that the auto-picking is more than 1,000 times more efficient than standard manual picking and the estimated velocities are in good agreement with available geotechnical and geophysical information. We conclude that the semi-automatic approach may represent a fast and straightforward method suitable for both research and industrial projects, thus enhancing further collaborations and developments

Formoterol Exerts Anti-Cancer Effects Modulating Oxidative Stress and Epithelial-Mesenchymal Transition Processes in Cigarette Smoke Extract Exposed Lung Adenocarcinoma Cells

Lung cancer frequently affects patients with Chronic Obstructive Pulmonary Disease (COPD). Cigarette smoke (CS) fosters cancer progression by increasing oxidative stress and by modulating epithelial-mesenchymal transition (EMT) processes in cancer cells. Formoterol (FO), a long-acting β2-agonist widely used for the treatment of COPD, exerts antioxidant activities. This study explored in a lung adenocarcinoma cell line (A549) whether FO counteracted the effects of cigarette smoke extract (CSE) relative to oxidative stress, inflammation, EMT processes, and cell migration and proliferation. A549 was stimulated with CSE and FO, ROS were evaluated by flow-cytometry and by nanostructured electrochemical sensor, EMT markers were evaluated by flow-cytometry and Real-Time PCR, IL-8 was evaluated by ELISA, cell migration was assessed by scratch and phalloidin test, and cell proliferation was assessed by clonogenic assay. CSE significantly increased the production of ROS, IL-8 release, cell migration and proliferation, and SNAIL1 expression but significantly decreased E-cadherin expression. FO reverted all these phenomena in CSE-stimulated A549 cells. The present study provides intriguing evidence that FO may exert anti-cancer effects by reverting oxidative stress, inflammation, and EMT markers induced by CS. These findings must be validated in future clinical studies to support FO as a valuable add-on treatment for lung cancer management

does a mediterranean model of family functioning in the perception of italian and spanish adolescents exist a cross national study

The current study explored the influence of belonging to a different areas, South of Italy and Spain, on perception of parenting and family functioning adolescents in order to highlight the similarities and differences among two samples and verify if Mediterranean model of family exists". Empirical evidence of the existence of a Mediterranean family model has been conducted to verify which common and differentiating features exist in the perception of family ties among adolescents. Data highlighted that adolescents belonging to these areas report similar levels in different component of familial ties that can be defined as "Links and Roles": On the contrary, important differences were underlined on the perception of levels of rigidity and enmeshment within family: on the base of Marcia's model of identity status, we called this dimension "Foreclosure status". DOI: 10.5901/mjss.2014.v5n1p37

Comparative Analysis of Five Multiplex RT-PCR Assays in the Screening of SARS-CoV-2 Variants

The rapid and presumptive detection of SARS-CoV-2 variants may be performed using multiplex RT-PCR assays. The aim of this study was to evaluate the diagnostic performance of five qualitative RT-PCR tests as compared with next-generation sequencing (NGS). We retrospectively examined a multi-variant panel (n = 72) of SARS-CoV-2-positive nasopharyngeal swabs categorized as variants of concern (Alpha, Beta, Gamma and Delta), variants under monitoring (Iota and Kappa) and wild-type strains circulating in Liguria (Italy) from January to August 2021. First, NGS libraries of study samples were prepared and mapped to the reference genome. Then, specimens were screened for the detection of L452R, W152C, K417T, K417N, E484Q, E484K and N501Y mutations using the SARS-CoV-2 Variants II Assay Allplex, UltraGene Assay SARS-CoV-2 452R & 484K & 484Q Mutations V1, COVID-19 Ultra Variant Catcher, SARS-CoV-2 Extended ELITe MGB and Simplexa SARS-CoV-2 Variants Direct. The overall accuracy of these assays ranged from 96.9% to 100%. Specificity and sensitivity were 100% and 96-100%, respectively. We highly recommend the use of these assays as second-level tests in the routine workflow of SARS-CoV-2 laboratory diagnostics, as they are accurate, user friendly, low cost, may identify specific mutations in about 2-3 h and, therefore, optimize the surveillance of SARS-CoV-2 variants

Gold nanowires-based sensor for quantification of H2O2 released by human airway epithelial cells

Hydrogen peroxide (H2O2) is a biomarker relevant for oxidative stress monitoring. Most chronic airway diseases are characterized by increased oxidative stress. To date, the main methods for the detection of this analyte are expensive and time-consuming laboratory techniques such as fluorometric and colorimetric assays. There is a growing interest in the development of electrochemical sensors for H2O2 detection due to their low cost, ease of use, sensitivity and rapid response. In this work, an electrochemical sensor based on gold nanowire arrays has been developed. Thanks to the catalytic activity of gold against hydrogen peroxide reduction and the high surface area of nanowires, this sensor allows the quantification of this analyte in a fast, efficient and selective way. The sensor was obtained by template electrodeposition and consists of gold nanowires about 5 high and with an average diameter of about 200 nm. The sensor has excellent properties in terms of reproducibility, repeatability and selectivity. The sensor was validated by quantifying the hydrogen peroxide released by human airways A549 cells exposed or not to the pro-oxidant compound rotenone. The obtained results were validated by comparing them with those obtained by flow cytometry after staining the cells with the fluorescent superoxide-sensitive Mitosox Red probe giving a very good concordance