Tratamiento y control de la diabetes con intervención de estilo de vida en pacientes ambulatorios: Serie de Casos y Actualización: Diabetes treatment and control using lifestyle intervention in outpatient setting: Case Series and Update

The prevalence of diabetes demands the identification of more efficient long-term methods to achieve good patient control. This paper reports the progress of 4 patients diagnosed with previously poorly controlled diabetes and prediabetes with comorbidities that were attended in an outpatient clinic, they received orientation regarding lifestyle change and were instructed to increase consumption of dietary fiber and physical activity. Body composition and biochemical markers were followed and changes were reported. In a 5 month period Patient 1 achieved criterion for controlled diabetes and was able to suspend oral hypoglycemic agents, reducing his body weight by 10%. Within a 8 week period Patient 2 experienced a 50% decrease of HOMA IR and was able to achieve criteria of controlled diabetes. Furthermore a 3 kg increase in lean body mass and a 4 kg body weight decrease were documented. These findings were accompanied by a 23% and 38% decrease in total cholesterol and triglycerides respectively. Patient 3 achieved criteria for type 2 diabetes remission within a 18 month time span(Inicial HBA1C 11.4% Final HBA1C 5.3). Patient 4 progressed to remission of prediabetes and a decrease of 5.9% in total body weight in a 3 month period.Las cifras de prevalencia de la diabetes nos obligan a identificar métodos más eficientes a largo plazo para lograr el buen control de los pacientes. En este trabajo se reporta el curso clínico de 4 pacientes con diagnóstico de diabetes y prediabetes quienes fueron atendidos en la consulta externa y recibieron asistencia no intensiva para cambio de comportamiento en salud, orientada a aumentar consumo de fibra alimentaria y a aumentar sus niveles de actividad física.  Se reportan cambios en indicadores bioquímicos y antropométricos. El paciente 1 en 5 meses logró suspender hipoglucemiantes orales, disminuyendo un 10% de su peso corporal y logrando criterios de prediabetes (remisión parcial). El paciente 2 en un período de 8 semanas logró alcanzar criterios de diabetes controlada, disminuyendo en 50% el índice HOMA IR.  Además, se documentó pérdida de 4 kg de masa grasa corporal con ganancia de 3 kg de masa magra.  Lo anterior se acompañó de una disminución del 23% del colesterol total y 38% de triglicéridos. La paciente 3 alcanzó criterios de remisión de DM2 durante un seguimiento de 18 meses(HBA1C 11.4% inicial y 5.3% final). La paciente 4 evolucionó a remisión de prediabetes y disminución de 5.9% de peso corporal total en un periodo de 3 meses

Superhydrophobic supported Ag-NPs@ZnO-nanorods with photoactivity in the visible range

In this article we present a new type of 1D nanostructures consisting of supported hollow ZnO nanorods (NRs) decorated with Ag nanoparticles (NPs). The 3D reconstruction by high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM) electron tomography reveals that the Ag NPs are distributed along the hollow interior of the ZnO NRs. Supported and vertically aligned Ag-NPs@ZnO-NRs grow at low temperature (135 °C) by plasma enhanced chemical vapour deposition on heterostructured substrates fabricated by sputtered deposition of silver on flat surfaces of Si wafers, quartz slides or ITO. The growth mechanisms of these structures and their wetting behavior before and after visible light irradiation are critically discussed. The as prepared surfaces are superhydrophobic with water contact angles higher than 150°. These surfaces turn into superhydrophilic with water contact angles lower than 10° after prolonged irradiation under both visible and UV light. The evolution rate of the wetting angle and its dependence on the light characteristics are related to the nanostructure and the presence of silver embedded within the ZnO NRs. ÂEuropean Union NMP3-CT-2006- 032583Ministerio de Ciencia e Innovación MAT2010-21228, MAT2010-18447, CSD2008-00023Junta de Andalucía P09-TEP-5283, CTS-518

SPOC: A widely distributed domain associated with cancer, apoptosis and transcription

BACKGROUND: The Split ends (Spen) family are large proteins characterised by N-terminal RNA recognition motifs (RRMs) and a conserved SPOC (Spen paralog and ortholog C-terminal) domain. The aim of this study is to characterize the family at the sequence level. RESULTS: We describe undetected members of the Spen family in other lineages (Plasmodium and Plants) and localise SPOC in a new domain context, in a family that is common to all eukaryotes using profile-based sequence searches and structural prediction methods. CONCLUSIONS: The widely distributed DIO (Death inducer-obliterator) family is related to cancer and apoptosis and offers new clues about SPOC domain functionality

Synthesis and Structural Analysis of Push-Pull Imidazole-Triazole Based Fluorescent Bifunctional Chemosensor for CU2+ and FE2+ Detection

[Abstract] Fluorescent imidazole-triazole based ligands L1 and L2 have been designed as chemical push-pull chemosensors for divalent metal ions and synthesized through palladium-catalyzed cross-coupling reactions using indium organometallics and click chemistry. The novel ligands exhibit intense absorption in the ultraviolet region with high molar extinction coefficients, and strong fluorescence emission with large Stokes displacements. On the basis of UV–Vis absorption spectroscopy and fluorescence emission data in acetonitrile, L1 is shown as a bifuncional chemosensor with differential response for Fe2+ and Cu2+ over a range of selected 3d divalent and other metal ions. The binding site of the ligand was established by single-crystal X-ray diffraction and 1H NMR spectroscopy studies. The association constants, determined by spectrofluorimetric titrations, show a steady binding affinity of L1 for Cu2+ and Fe2+ in comparison with other previously reported fluorescent bidentate chemosensors, offering the lowest limit of detection (LOD) with Cu2+. DFT calculations provide a rationale properly understanding and interpreting the experimentally observed results. Indeed, a mechanism of the different optical responses of L1 towards 3d divalent metal ions is proposed.We thank the Spanish Ministerio de Ciencia, Innovación y Universidades (PGC2018-097792-B-I00), Xunta de Galicia (GRC2018/039 and ED431B 2020/52) and EDRF funds for financial and human support. ADL thanks the Xunta de Galicia for a predoctoral fellowship (EDA 481A-2020/017). LV thanks CACTI for X-ray measurementsXunta de Galicia; GRC2018/039Xunta de Galicia; ED431B 2020/52Xunta de Galicia; ED 481A-2020/01

Plasma assisted deposition of single and multistacked TiO2 hierarchical nanotubes photoanodes

We present herein an evolved methodology for the growth of nanocrystalline hierarchical nanotubes combining physical vapor deposition of organic nanowires (ONWs) and plasma enhanced chemical vacuum deposition of anatase TiO2 layers. The ONWs act as vacuum removable 1D and 3D templates, with the whole process occurring at temperatures ranging from RT to 250 °C. As a result, a high density of hierarchical nanotubes with tunable diameter, length and tailored wall microstructures are formed on a variety of processable substrates as metal and metal oxide films or nanoparticles including transparent conductive oxides. The reiteration of the process leads to the development of an unprecedented 3D nanoarchitecture formed by stacking the layers of hierarchical TiO2 nanotubes. As a proof of concept, we present the superior performance of the 3D nanoarchitecture as a photoanode within an excitonic solar cell with efficiencies as high as 4.69% for a nominal thickness of the anatase layer below 2.75 ¿m. Mechanical stability and straightforward implementation in devices are demonstrated at the same time. The process is extendable to other functional oxides fabricated by plasma-assisted methods with readily available applications in energy harvesting and storage, catalysis and nanosensingJunta de Andalucia(FQM 1851 and FQM-2310)España Mineco 201560E055 MAT2016-79866-R MAT2013-40852-R MAT2013-4MAT2013-47192-C3-3-R2900-

Complex Variant t(9;22) Chromosome Translocations in Five Cases of Chronic Myeloid Leukemia

The Philadelphia (Ph1) chromosome arising from the reciprocal t(9;22) translocation is found in more than 90% of chronic myeloid leukemia (CML) patients and results in the formation of the chimeric fusion gene BCR-ABL. However, a small proportion of patients with CML have simple or complex variants of this translocation, involving various breakpoints in addition to 9q34 and 22q11. We report five CML cases carrying variant Ph translocations involving both chromosomes 9 and 22 as well as chromosomes 3, 5, 7, 8, or 10. G-banding showed a reciprocal three-way translocation involving 3q21, 5q31, 7q32, 8q24, and 10q22 bands. BCR-ABL fusion signal on der(22) was found in all of the cases by FISH

Cholesterol-Metabolizing Enzyme Cytochrome P450 46A1 as a Pharmacologic Target for Alzheimer’s Disease

Cytochrome P450 46A1 (CYP46A1 or cholesterol 24-hydroxylase) controls cholesterol elimination from the brain and plays a role in higher order brain functions. Genetically enhanced CYP46A1 expression in mouse models of Alzheimer's disease mitigates the manifestations of this disease. We enhanced CYP46A1 activity pharmacologically by treating 5XFAD mice, a model of rapid amyloidogenesis, with a low dose of the anti-HIV medication efavirenz. Efavirenz was administered from 1 to 9 months of age, and mice were evaluated at specific time points. At one month of age, cholesterol homeostasis was already disturbed in the brain of 5XFAD mice. Nevertheless, efavirenz activated CYP46A1 and mouse cerebral cholesterol turnover during the first four months of administration. This treatment time also reduced amyloid burden and microglia activation in the cortex and subiculum of 5XFAD mice as well as protein levels of amyloid precursor protein and the expression of several genes involved in inflammatory response. However, mouse short-term memory and long-term spatial memory were impaired, whereas learning in the context-dependent fear test was improved. Additional four months of drug administration (a total of eight months of treatment) improved long-term spatial memory in the treated as compared to the untreated mice, further decreased amyloid-β content in 5XFAD brain, and also decreased the mortality rate among male mice. We propose a mechanistic model unifying the observed efavirenz effects. We suggest that CYP46A1 activation by efavirenz could be a new anti-Alzheimer's disease treatment and a tool to study and identify normal and pathological brain processes affected by cholesterol maintenance

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Objetivo: caracterizar los eventos adversos presentados en una E.S.E de primer nivel en Caldas entre 2007 y  2009. Materiales y métodos: estudio descriptivo de corte transversal, retrospectivo, que analizó la  información obtenida de los eventos adversos (E.A) registrados en los años 2007 a 2009 en una institución de  primer nivel de Caldas. Se revisó la base de datos de reporte de E.A de la institución, que contenía 112  registros; se descartaron 63 registros y se seleccionaron los que podían ser evaluados a partir de la historia  clínica (49). Se les aplicó un instrumento a través del cual se identificaron variables socio-demográficas,  características del evento y calidad del registro. Resultados: de los E.A registrados, 38 corresponden al sexo  femenino y 11 al sexo masculino, los E.A más frecuentes fueron los obstétricos (40,8%), muerte por  inatención (16,3%), los menos frecuentes fueron fallos en la seguridad (2,0%); según gravedad, 18,4%  muertes; 57,1% leves y 8.2% moderados. En el 30,6% de los casos no se instauró manejo, en el 22,4% pequeña cirugía y otros manejos 20,4%. Con respecto a la calidad del registro, el 32,6% eran malos, el 44,8% regulares  y el 20,4% eran de buena calidad. Conclusiones: el 100% de los eventos adversos no contaban con actas  individuales de seguimiento, solo se cuenta con un acta de reporte de los eventos adversos más relevantes,  pero estas no implican un seguimiento del evento, ni contienen los datos específicos de la situación ocurrida  y del paciente.Objective: to characterize the adverse events happening in a first-level hospital in the Department of Caldas  between 2007 and 2009. Materials and methods: a cross-sectional retrospective descriptive study was  performed which analyzed the information obtained during the adverse events (AE) registered between 2007 and 2009 in a first level hospital in the Department of Caldas. An EA data report of the institution was revised  which contained 112 records. 63 records were highlighted and those that could be evaluated from the  medical record (49) were selected. They were applied an instrument though which social-demographic,  characteristics of the event and quality of the record variables were identified. Results: from the AE recorded, 38 cases corresponded to females and 11 cases to males. The most frequent AE were obstetric events (40.8%) and death as a result of lack of attention (16.3%); the least frequent cases were failures in safety (2.0%);  18.4% were deaths depending on seriousness of disease, 57.1% were slight events and 8.2% were moderate  events. In 30.6% of the cases management was not established; in 22.4% of the cases small surgery was  applied and in 20.4% other procedures were applied. As far as quality of records is concerned, 32.6% were  bad, 44.8% were average, and 20.4% were high quality. Conclusions: 100% of the adverse events did not have individual proceeding records. Only one report of the most relevant AE was available but it neither implies a  follow up of the event nor contains specific data of the situation or the patient

The Role of Surface Recombination on the Performance of Perovskite Solar Cells:Effect of Morphology and Crystalline Phase of TiO₂ Contact

Herein, the preparation of 1D TiO2 nanocolumnar films grown by plasma-enhanced chemical vapor deposition is reported as the electron selective layer (ESL) for perovskite solar devices. The impact of the ESL architecture (1D and 3D morphologies) and the nanocrystalline phase (anatase and amorphous) is analyzed. For anatase structures, similar power conversion efficiencies are achieved using an ESL either the 1D nanocolumns or the classical 3D nanoparticle film. However, lower power conversion efficiencies and different optoelectronic properties are found for perovskite devices based on amorphous 1D films. The use of amorphous TiO2 as electron selective contact produces a bump in the reverse scan of the current–voltage curve as well as an additional electronic signal, detected by impedance spectroscopy measurements. The dependence of this additional signal on the optical excitation wavelength used in the IS experiments suggests that it stems from an interfacial process. Calculations using a drift-diffusion model which explicitly considers the selective contacts reproduces qualitatively the main features observed experimentally. These results demonstrate that for a solar cell in which the contact is working properly the open-circuit photovoltage is mainly determined by bulk recombination, whereas the introduction of a “bad contact” shifts the balance to surface recombination.</p