13 research outputs found
Optimization of transfection methods for HuhΒ7 and Vero cells: a comparative study
Availability of an efficient transfection protocol is the first determinant in success of gene transferring studies in mammalian cells which is accomplished experimentally for every single cell type. Herein, we provide data of a comparative study on optimization of transfection condition by electroporation and chemical methods for Huh-7 and Vero cells. Different cell confluencies, DNA/reagent ratios and total transfection volumes were optimized for two chemical reagents including jetPEIβ’ and Lipofectamineβ’ 2000. Besides, the effects of electric field strength and pulse length were investigated to improve electroporation efficiency. Transfection of cells by pEGFP-N1 vector and tracking the expression of GFP by FACS and Fluorescence Microscopy analysis were the employed methods to evaluate transfection efficiencies. Optimized electroporation protocols yielded 63.73 Β± Β± 2.36 and 73.9 Β± 1.6 % of transfection in Huh-7 and Vero cells respectively, while maximum achieved level of transfection by jetPEIβ’ was respectively 14.2 Β± 0.69 and 28 Β± 1.11 % for the same cells. Post transfectional chilling of the cells did not improve electrotransfection efficiency of Huh-7 cells. Compared to chemical based reagents, electroporation showed the superior levels of transfection in both cell lines. The presented protocols should satisfy most of the experimental applications requiring high transfection efficiencies of these two cell lines.ΠΠ°Π»ΠΈΡΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Π° ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠ΅ΡΠ²ΡΠΌ ΡΡΠ»ΠΎΠ²ΠΈΠ΅ΠΌ ΡΡΠΏΠ΅ΡΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΠΎ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΡ Π³Π΅Π½ΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ
, ΡΡΠΎ Π΄ΠΎΡΡΠΈΠ³Π°Π΅ΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎ Π΄Π»Ρ ΠΊΠ°ΠΆΠ΄ΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΏΠ° ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΠ΄Π΅ΡΡ ΠΌΡ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠΌ Π΄Π°Π½Π½ΡΠ΅ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Huh-7 ΠΈ Vero Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΎΡΠ°ΡΠΈΠΈ ΠΈ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ. ΠΠ»Ρ Π΄Π²ΡΡ
Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, jetPEIβ’ ΠΈ Lipofectamineβ’ 2000, Π±ΡΠ»ΠΈ ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Ρ ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ, ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΠΠ/ΡΠ΅Π°Π³Π΅Π½Ρ ΠΈ ΠΎΠ±ΡΠΈΠ΅ ΠΎΠ±ΡΠ΅ΠΌΡ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π΄Π»Ρ ΡΠ»ΡΡΡΠ΅Π½ΠΈΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΎΡΠ°ΡΠΈΠΈ Π±ΡΠ»ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΎ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠΈΠ»Ρ ΡΠ»Π΅ΠΊΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΠ»Ρ ΠΈ Π΄Π»ΠΈΠ½Ρ ΠΈΠΌΠΏΡΠ»ΡΡΠ°. Π’ΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π²Π΅ΠΊΡΠΎΡΠ° pEGFP-N1, ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ GFP Ρ ΠΏΠΎΠΌΠΎΡΡΡ FACS ΠΈ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½Π°Ρ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡ Π±ΡΠ»ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π΄Π»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ. Π ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Π°Ρ
Π΄ΠΎΡΡΠΈΠ³Π°Π»Π°ΡΡ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ Π½Π° ΡΡΠΎΠ²Π½Π΅ 63.73 Β± 2.36 ΠΈ 73.9 Β± 1.6 % Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
Huh-7 ΠΈ Vero ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ jetPEIβ’ ΡΠΎΡΡΠ°Π²Π»ΡΠ» 14.2 Β± 0.69 ΠΈ 28 Β± 1.11 % Π΄Π»Ρ ΡΠ΅Ρ
ΠΆΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΡ
Π»Π°ΠΆΠ΄Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎΡΠ»Π΅ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ Π½Π΅ ΡΠ»ΡΡΡΠ°Π»ΠΎ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠ»Π΅ΠΊΡΡΠΎΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Huh-7. Π ΠΎΠ±Π΅ΠΈΡ
ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
Π»ΠΈΠ½ΠΈΡΡ
ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΎΡΠ°ΡΠΈΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° Π΄ΠΎΡΡΠΈΡΡ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ². ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΠΏΡΠΎΡΠΎΠΊΠΎΠ» ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΏΡΠΈΠ³ΠΎΠ΄Π½ΡΠΌ Π΄Π»Ρ Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π° ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΌΠ°Π½ΠΈΠΏΡΠ»ΡΡΠΈΠΉ, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΡΠ΅Π±ΡΡΡ Π²ΡΡΠΎΠΊΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
Π»ΠΈΠ½ΠΈΠΉ
The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men
Background: Human papilloma virus (HPV) infection is one of the most common sexually transmitted diseases that affects men like women and infected cutaneous and mucosal squamous epithelium. The aim of the present study was to determine the prevalence of HPV in the semen of oligospermic, azoospermic and normal patients. Methods: From June 2012 to June 2013, a total of 90 individuals were enrolled in this cross sectional comparative study. The participants were classified into three groups (oligospermia, azoosprmia and normal). This classification was based on a new WHO reference values for human semen characteristics published on 2010. After extraction of DNA from specimens L1 gene of HPV was amplified by nested polymerase chain reaction (Nested-PCR) and the PCR products of positive specimens were genotyped using INNO-LiPA HPV Genotyping Extra assay. Results: Among 50 confirmed oligospermic male, 15 were HPV DNA positive (30). In azoospemic group we had 8 HPV DNA positive (40) and in normal group just 3 of 20(15) samples were positive. Statistical assessment was done with SPSS v.15. Chi-square test showed no significant relationship between 3 groups results. Based on independent samples t-test, we found statistical significant relationship for sperm count (p<0.05) and sperm motility (slow) (p<0.05) in oligospermic group positive samples compared with negative. In the present study, 13 HPV genotypes were detected among positive samples. HPV genotypes 16, 45 in the high risk group and 6,11,42 in the low risk group were more frequent than the others. Conclusion: The current study shows that HPV infection can affect on sperm count and motility and decrease count of sperm cell and decrease motility capability of these cells
Design of Lentiviral Vector of Apoptin and Investigating its Cytotoxic Effect on Reh Acute Lymphoblastic Leukemia Cells
BACKGROUND AND OBJECTIVE: Resistance to chemotherapy drugs is one of the most important treatment problems in patients with acute lymphoblastic leukemia. Apoptin due to its ability to induce apoptosis in tumor cells, has an undeniable potential in cancer treatment, especially those that respond lessly to chemotherapy. Therefore, in this study, the effect of induction of apoptin expression on induction of cell death in reh malignant lymphoblasts was investigated.
METHODS: In this experimental study, after culturing of Reh cells (prepared by the Pasteur Institute), the entry of lentiviral vector, metabolic activity and cell proliferation were measured using flow cytometry, MTT and trypan blue at 24, 48, 72 and 96 hours. Real time PCR was also used to determine the apoptin gene expression.
FINDINGS: The results of this study indicate the design and construction of a suitable lentiviral vector that can infect more than 65% of the cells. Also, after infecting the cells with the vector, the apoptin gene expression rate was increased about 10 times to control, and subsequently, the cell viability decreased by 53% time-dependently (p <0.01) . The results also showed that the inhibitory effect of apoptin gene expression on the metabolic activity of Reh cells was about 35% (p<0.01).
CONCLUSION: The results of the study showed that apoptin gene expression has anti-tumor activity in Reh cells and can be used as a promising therapy in ALL
Prevalence of Merkel cell polyomavirus in Tehran: An age-specific serological study
Background: Several new types of polyomavirus have been discovered in recent years mainly because of the recent state-of-the-art detection technologies. Among the polyomaviruses, Merkel cell polyomavirus (MCPyV) has attracted the most attention because of its possible role in the etiology of Merkel cell carcinoma, a rare but lethal form of skin cancer. Objectives: This study aimed to determine age-specific seroprevalence of MCPyV in Tehran. Patients and Methods: In this cross-sectional study, we collected 440 serum samples from healthy individuals 2 to 78 years of age who visited the Pasteur InstituteΓ’οΏ½οΏ½s clinic in Tehran, Iran, using a convenience sampling strategy. We developed a virus-like particle-based enzyme-linked immunosorbent assay that uses VP1, the major capsid protein of MCPyV, to detect and quantitate serum antibodies to MCPyV. We compared the prevalence of MCPyV between males and females and across eight age groups. Results: A total of 255 (57.9) of the serum samples were MCPyV positive. The seroprevalence in children under 10 years of age was 25. The seroprevalence increased to 56 over the next decade of life (10 - 19 years of age). The seroprevalence rate in males and females was 56.1 and 59.7 respectively, and a binary logistic regression showed no significant difference between males and females (P = 0.77). However, the prevalence of MCPyV increased with age (P = 0.012). Conclusions: Our results suggest that human exposure to MCPyV occurs throughout life. The MCPyV antibody levels remained high among older adults in our population, consistent with reports from other populations. ΓΒ© 2016, Iranian Red Crescent Medical Journal
C-Terminal Domain Deletion Enhances the Protective Activity of cpa/cpb Loaded Solid Lipid Nanoparticles against Leishmania major in BALB/c Mice
Cutaneous leishmaniasis (CL) is the most common form of leishmaniasis with an annual incidence of approximately 2 million cases and is endemic in 88 countries, including Iran. CL's continued spread, along with rather ineffectual treatments and drug-resistant variants emergence has increased the need for advanced preventive strategies. We studied Type II cysteine proteinase (CPA) and Type I (CPB) with its C-terminal extension (CTE) as cocktail DNA vaccine against murine and canine leishmaniasis. However, adjuvants' success in enhancing immune responses to selected antigens led us to refocus our vaccine development programs. Herein, we discuss cationic solid lipid nanoparticles' (cSLN) ability to improve vaccine-induced protective efficacy against CL and subsequent lesion size and parasite load reduction in BALB/c mice. For this work, we evaluated five different conventional as well as novel parasite detection techniques, i.e., footpad imaging, footpad flowcytometry and lymph node flowcytometry for disease progression assessments. Vaccination with cSLN-cpa/cpb-CTE formulation showed highest parasite inhibition at 3-month post vaccination. Immunized mice showed reduced IL-5 level and significant IFN-Γ£ increase, compared to control groups. We think our study represents a potential future and a major step forward in vaccine development against leishmaniasis
Optimization of transfection methods for Huh-7 and Vero cells: A comparative study
Availability of an efficient transfection protocol is the first determinant in success of gene transferring studies in mammalian cells which is accomplished experimentally for every single cell type. Herein, we provide data of a comparative study on optimization of transfection condition by electroporation and chemical methods for Huh-7 and Vero cells. Different cell confluencies, DNA/reagent ratios and total transfection volumes were optimized for two chemical reagents including jetPEIβ’ and Lipofectamineβ’ 2000. Besides, the effects of electric field strength and pulse length were investigated to improve electroporation efficiency. Transfection of cells by pEGFP-N1 vector and tracking the expression of GFP by FACS and Fluorescence Microscopy analysis were the employed methods to evaluate transfection efficiencies. Optimized electroporation protocols yielded 63.73 Β± Β± 2.36 and 73.9 Β± 1.6 % of transfection in Huh-7 and Vero cells respectively, while maximum achieved level of transfection by jetPEIβ’ was respectively 14.2 Β± 0.69 and 28 Β± 1.11 % for the same cells. Post transfectional chilling of the cells did not improve electrotransfection efficiency of Huh-7 cells. Compared to chemical based reagents, electroporation showed the superior levels of transfection in both cell lines. The presented protocols should satisfy most of the experimental applications requiring high transfection efficiencies of these two cell lines.ΠΠ°Π»ΠΈΡΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Π° ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠ΅ΡΠ²ΡΠΌ ΡΡΠ»ΠΎΠ²ΠΈΠ΅ΠΌ ΡΡΠΏΠ΅ΡΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΠΎ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΡ Π³Π΅Π½ΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ
, ΡΡΠΎ Π΄ΠΎΡΡΠΈΠ³Π°Π΅ΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎ Π΄Π»Ρ ΠΊΠ°ΠΆΠ΄ΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΏΠ° ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΠ΄Π΅ΡΡ ΠΌΡ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠΌ Π΄Π°Π½Π½ΡΠ΅ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Huh-7 ΠΈ Vero Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΎΡΠ°ΡΠΈΠΈ ΠΈ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ. ΠΠ»Ρ Π΄Π²ΡΡ
Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, jetPEIβ’ ΠΈ Lipofectamineβ’ 2000, Π±ΡΠ»ΠΈ ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Ρ ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ, ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΠΠ/ΡΠ΅Π°Π³Π΅Π½Ρ ΠΈ ΠΎΠ±ΡΠΈΠ΅ ΠΎΠ±ΡΠ΅ΠΌΡ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π΄Π»Ρ ΡΠ»ΡΡΡΠ΅Π½ΠΈΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΎΡΠ°ΡΠΈΠΈ Π±ΡΠ»ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΎ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠΈΠ»Ρ ΡΠ»Π΅ΠΊΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΠ»Ρ ΠΈ Π΄Π»ΠΈΠ½Ρ ΠΈΠΌΠΏΡΠ»ΡΡΠ°. Π’ΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π²Π΅ΠΊΡΠΎΡΠ° pEGFP-N1, ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ GFP Ρ ΠΏΠΎΠΌΠΎΡΡΡ FACS ΠΈ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½Π°Ρ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡ Π±ΡΠ»ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π΄Π»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ. Π ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Π°Ρ
Π΄ΠΎΡΡΠΈΠ³Π°Π»Π°ΡΡ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ Π½Π° ΡΡΠΎΠ²Π½Π΅ 63.73 Β± 2.36 ΠΈ 73.9 Β± 1.6 % Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
Huh-7 ΠΈ Vero ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ jetPEIβ’ ΡΠΎΡΡΠ°Π²Π»ΡΠ» 14.2 Β± 0.69 ΠΈ 28 Β± 1.11 % Π΄Π»Ρ ΡΠ΅Ρ
ΠΆΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΡ
Π»Π°ΠΆΠ΄Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎΡΠ»Π΅ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ Π½Π΅ ΡΠ»ΡΡΡΠ°Π»ΠΎ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠ»Π΅ΠΊΡΡΠΎΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Huh-7. Π ΠΎΠ±Π΅ΠΈΡ
ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
Π»ΠΈΠ½ΠΈΡΡ
ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΎΡΠ°ΡΠΈΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° Π΄ΠΎΡΡΠΈΡΡ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ². ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΠΏΡΠΎΡΠΎΠΊΠΎΠ» ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΏΡΠΈΠ³ΠΎΠ΄Π½ΡΠΌ Π΄Π»Ρ Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π° ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΌΠ°Π½ΠΈΠΏΡΠ»ΡΡΠΈΠΉ, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΡΠ΅Π±ΡΡΡ Π²ΡΡΠΎΠΊΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
Π»ΠΈΠ½ΠΈΠΉ