EXPERIMENTAL DERMATOPHYTOSIS ON THE OUTER EAR OF GUINEA PIGS: A MODEL THAT MIMICS NATURAL INFECTIONdoi: http://dx.doi.org/10.5892/ruvrd.v12i1.1359

In the present study a new model of experimental dermatophytosis was tested. The aim was to achieve maximum similarity to a natural infection through a procedure that is easy to carry out. The experimental infection was induced by a single inoculation of 0.5 mL of a suspension with hyphal fragments of T. mentagrophytes var. mentagrophytes on the outer ear of guinea pigs. The first signs of infection were observe on the seventh day after inoculation. The experiment demonstrated that this method, besides being effective, causes less stress and the clinical aspects the lesions here were similar to natural lesions

Effect of a hyperlipidic diet rich in omegas 3, 6 and 9 on aberrant crypt formation in rat colonic mucosa Efeito de uma dieta hiperlipídica rica em ômegas 3, 6 e 9 na formação de criptas aberrantes em mucosa cólica de ratos

PURPOSE: To determine whether a hypercaloric and hyperlipidic diet enriched with polyunsaturated fatty acids influences the formation of aberrant crypt foci (ACF) in colonic mucosa of Wistar rats treated with azoxymethane (AOM). METHODS: At eight weeks of life, the rats were assigned to four groups: Group I&#8213;standard diet (STD) not treated with AOM; Group II&#8213;hypercaloric and hyperlipidic diet (FED), not treated with AOM; Group III&#8213;STD, treated with AOM; Group IV&#8213;FED, treated with AOM. At 16 weeks, the animals were injected intraperitoneal with 0.9% saline solution (Group I and II) or AOM at 15mg/Kg (Groups III and IV) once a week for two weeks. Fifteen weeks later, the animals were euthanized. RESULTS: FED promoted weight gain in Groups II and IV compared to Groups I and III, respectively. The groups did not differ with regard to the total number of ACF. The Chi-square test revealed no predominance of the presence of foci with <4 crypts. However, foci with &#8805;5 crypts were proportionally more prevalent in Group III than in Group IV (p=0.043). CONCLUSION: The administration of polyunsaturated fatty acids did not interfere with the formation of aberrant crypt foci, but reduced ACF multiplicity, exercising an attenuating effect on carcinogenesis.<br>OBJETIVO: Determinar se uma dieta hipercalórica, hiperlipídica, rica em ácidos graxos poliinsaturados (FED) tem influência na formação de focos de cripta aberrante (FCA) em mucosa cólica de ratos Wistar expostos ao azoximetano (AOM). MÉTODOS: Com oito semanas de vida, os ratos foram distribuídos em quatro grupos: Grupo I: Dieta padrão (SD) sem AOM; Grupo II: FED, sem AOM; Grupo III: SD, com AOM; Grupo IV: FED com AOM. Com 16 semanas, os animais dos grupos I e II receberam injeções intraperitoneais de solução salina 0,9%, enquanto os dos grupos III e IV receberam AOM na dose de 15mg/Kg de peso, 1 vez por semana por duas semanas. Quinze semanas após, os animais foram mortos. RESULTADOS: FED promoveu aumento de peso nos grupos II e IV em relação aos grupos I e III. Não houve aumento significante no número total de FCA entre os grupos. Em relação à multiplicidade das criptas por FCA, o teste do qui-quadrado mostrou que não houve predominância da presença <4 criptas por foco. Contudo, focos &#8805;5 criptas foram proporcionalmente mais prevalentes no grupo III que no grupo IV (p=0,043). CONCLUSÃO: Os ácidos graxos poliinsaturados não interferem na formação de focos de cripta aberrante, contudo reduz sua multiplicidade, exercendo efeito atenuador na carcinogênese

Experimental dermatophytosis on the outer ear of guinea pigs: A model that mimics natural infection

In the present study a new model of experimental dermatophytosis was tested. The aim was to achieve maximum similarity to a natural infection through a procedure that is easy to carry out. The experimental infection was induced by a single inoculation of 0.5 mL of a suspension with hyphal fragments of T. mentagrophytes var. mentagrophytes on the outer ear of guinea pigs. The first signs of infection were observe on the seventh day after inoculation. The experiment demonstrated that this method, besides being effective, causes less stress and the clinical aspects the lesions here were similar to natural lesions

Bacillary angiomatosis in HIV-positive patient from Northeastern Brazil: a case report

It is a report of disseminated bacillary angiomatosis (BA) in a 23-year-old female patient, who is HIV-positive and with fever, weight loss, hepatomegaly, ascites, and papular-nodular skin lesions. The clinical and diagnostic aspects involved in the case were discussed. Bacillary angiomatosis must always be considered in the diagnosis of febrile cutaneous manifestations in AIDS