THE OLDER GENERATION IN THE TRADITIONAL CULTURE OF THE MIDDLE VOLGA REGION

В статье рассматривается культурный потенциал старшего поколения и его место в традиционном обществе; анализируется роль стариков в традиционных крестьянских семьях у русских, мордвы и чувашей; показывается значение «третьего возраста» в функционировании механизма сохранения и передачи социокультурных традиций.The article examines the cultural potential of the older generation and its place in traditional society; it analyzes the role of elders in traditional peasant families in the traditional peasant family of Russian, Mordovians and Chuvash; it shows the importance of the “third age” in the functioning of the mechanism of preservation and transmission of social and cultural traditions

HLA homozygosity does not adversely affect measles vaccine-induced cytokine responses

AbstractThe association between HLA homozygosity and measles-specific Th1 (IFN-γ, IL-2 and IL-12p40) and Th2 (IL-4 and IL-10) cytokine responses were assessed in a group of 339 healthy schoolchildren 12–18 years of age previously immunized with two doses of live-attenuated measles virus vaccine. No associations were observed between class I HLA homozygosity and measles-specific cytokine levels. Children who were homozygous at the class II DRB1, DQA1, DPA1 and DPB1 loci had higher median IFN-γ secretion levels compared with children who were heterozygous for DRB1 (77.7 vs. 39.5 pg/ml, p=0.05), DQA1 (60.9 vs. 36.6 pg/ml, p=0.03), DPA1 (46.1 vs. 27.1 pg/ml, p=0.01) and DPB1 (61.5 vs. 36.0 pg/ml, p=0.01) loci, respectively. Homozygosity at increasing numbers of HLA loci ( >=4) was associated with increased IFN-γ secretion levels (test for trend p-value=0.01). Our results suggest that HLA homozygosity showed no disadvantage for measles-specific cytokine responses and instead was associated with increased IFN-γ levels

Associations between SNPs in candidate immune-relevant genes and rubella antibody levels: a multigenic assessment

<p>Abstract</p> <p>Background</p> <p>The mechanisms of immune response are structured within a highly complex regulatory system. Genetic associations with variation in the immune response to rubella vaccine have typically been assessed one locus at a time. We simultaneously assessed the associations between 726 SNPs tagging 84 candidate immune response genes and rubella-specific antibody levels. Blood samples were obtained from 714 school-aged children who had received two doses of MMR vaccine. Associations between rubella-specific antibody levels and 726 candidate tagSNPs were assessed both one SNP at a time and in a variety of multigenic analyses.</p> <p>Results</p> <p>Single-SNP assessments identified 4 SNPs that appeared to be univariately associated with rubella antibody levels: rs2844482 (p = 0.0002) and rs2857708 (p = 0.001) in the 5'UTR of the LTA gene, rs7801617 in the 5'UTR of the IL6 gene (p = 0.0005), and rs4787947 in the 5'UTR of the IL4R gene (p = 0.002). While there was not significant evidence in favor of epistatic genetic associations among the candidate SNPs, multigenic analyses identified 29 SNPs significantly associated with rubella antibody levels when selected as a group (p = 0.017). This collection of SNPs included not only those that were significant univariately, but others that would not have been identified if only considered in isolation from the other SNPs.</p> <p>Conclusions</p> <p>For the first time, multigenic assessment of associations between candidate SNPs and rubella antibody levels identified a broad number of genetic associations that would not have been deemed important univariately. It is important to consider approaches like those applied here in order to better understand the full genetic complexity of response to vaccination.</p

THE ROLE OF THE STUDY OF NATIONAL CULTURE AND TRADITION IN THE AESTHETIC EDUCATION OF MODERN DESIGNERS

В статье представлена связь изобразительного, декоративно-прикладного и народного искусства в целях художественного образования и эстетического воспитания студентов на народных традицияхThe article presents the connection of fine, decorative, applied and folk art for the purpose of art education and aesthetic education of students on folk tradition

Extended LTA, TNF, LST1 and HLA Gene Haplotypes and Their Association with Rubella Vaccine-Induced Immunity

Recent studies have suggested the importance of HLA genes in determining immune responses following rubella vaccine. The telomeric class III region of the HLA complex harbors several genes, including lymphotoxin alpha (LTA), tumor necrosis factor (TNF) and leukocyte specific transcript -1 (LST1) genes, located between the class I B and class II DRB1 loci. Apart from HLA, little is known about the effect of this extended genetic region on HLA haplotypic backgrounds as applied to immune responses.We examined the association between immune responses and extended class I-class II-class III haplotypes among 714 healthy children after two doses of rubella vaccination. These extended haplotypes were then compared to the HLA-only haplotypes. The most significant association was observed between haplotypes extending across the HLA class I region, ten-SNP haplotypes, and the HLA class II region (i.e. A-C-B-LTA-TNF-LST1-DRB1-DQA1-DQB1-DPA1-DPB1) and rubella-specific antibodies (global p-value of 0.03). Associations were found between both extended A*02-C*03-B*15-AAAACGGGGC-DRB1*04-DQA1*03-DQB1*03-DPA1*01-DPB1*04 (p = 0.002) and HLA-only A*02-C*03-B*15-DRB1*04-DQA1*03-DQB1*03-DPA1*01-DPB1*04 haplotypes (p = 0.009) and higher levels of rubella antibodies. The class II HLA-only haplotype DRB1*13-DQA1*01-DQB1*06-DPA1*01-DPB1*04 (p = 0.04) lacking LTA-TNF-LST1 SNPs was associated with lower rubella antibody responses. Similarly, the class I-class II HLA-only A*01-C*07-B*08-DRB1*03-DQA1*05-DQB1*02-DPA1*01-DPB1*04 haplotype was associated with increased TNF-alpha secretion levels (p = 0.009). In contrast, the extended AAAACGGGGC-DRB1*01-DQA1*01-DQB1*05-DPA1*01-DPB1*04 (p = 0.01) haplotype was found to trend with decreased rubella-specific IL-6 secretion levels.These data suggest the importance of examining both HLA genes and genes in the class III region as part of the extended haplotypes useful in understanding genomic drivers regulating immune responses to rubella vaccine

FEATURES OF STEROID REGULATION OF CONGENITAL PROTECTIVE FACTORS AND OSTEOTROPIC MEDIATORS OF ORAL FLUID IN PREGNANT WOMEN WITH INFLAMMATORY PERIODONTAL DISEASES

Aim. To study changes in antimicrobial and osteotropic oral fluid mediators during pregnancy in women with chronic generalized periodontitis (CGР) of mild and moderate severity, taking into account the factor of steroid regulation by cortisol.Materials and methods. Among the 62 pregnant women (age 18 to 35 years), depending on the presence or absence of inflammatory periodontal diseases, the main group (n=31) with moderate and moderate CGР and control group (n=31) with no disease were formed. In addition, 32 healthy non-pregnant women (age 18 to 30 years) examined the oral fluid in the same diagnostic volume. In the oral liquid, the amount of catelicidin LL-37, osteoprotegerin and cortisol was determined using a solid-phase immunoassay method. Laboratory studies were conducted in I (8-12 weeks), II (13-27 weeks) and III (28-40 weeks) trimester of pregnancy.Results. In pregnant women with a physiologically pregnant pregnancy and in the absence of dental diseases in the oral fluid with a consequent increase in the concentration of cortisol, there was a decrease in the content of catelicidin LL-37 1 trimester followed by an increase in the 2nd and 3rd trimesters of the antimicrobial potential of the oral biological environment, an increase in the concentration of osteoprotegerin that inhibits the activity of osteoresorption in the alveolar bone. In pregnant women with CGR of mild and moderate severity, a marked increase in the concentration of cortisol in the oral fluid was accompanied in the first two terms by an increased content of cathelicidin LL-37 and activation of antimicrobial congenital mechanisms of resistance to pathogenic bacteria, followed by a decrease in the antimicrobial factor to the third trimester of the gestation period, a consistent decrease in the content of osteoprotegerin in mixed saliva.Conclusion. The presence of CGР of mild and moderate severity in pregnant women leads to a dissociation of the connection between cortisol mixed saliva and immune defense factors, osteotropic mediators in contrast to women who do not suffer from dental pathology during the gestational period

CONTENTS OF REGULATORY T-CELLS IN PERIPHERAL BLOOD AND ENDOMETRIAL TISSUE IN DYNAMIC OF MENSTRUAL CYCLE

The maintenance of CD4+CD25+/high and CD4+CD25+CD127 — regulatory T-cells in a peripheral, menstrual blood and an endometrial tissue in different phases of a menstrual cycle is investigated. It is shown that in a phase of average secretion the number of regulatory T-cells is increased. Thus quantity of CD4+CD25+increased in peripheral circulation, and in an endometrial tissue number of CD4+CD25+CD127- cells grew

THE USE OF ANTI-INFLAMMATORY EFFECT OF THE SORPTION COMPLEX IN REHABILITATION OF PATIENTS WITH CHRONIC HEART FAILURE AFTER MYOCARDIAL INFARCTION

The aim of the study was to investigate the anti-inflammatory effect of minimum dosages of enterosorbent containing carbon and aluminum oxide on the background of standard treatment for patients with chronic heart failure after myocardial infarction. After two weeks of receiving enterosorbent containing carbon and aluminum oxide in patients with CHF (n = 39) showed a reduction in the level of C-reactive protein, as well as marked improvement in microcirculation, which is a manifestation reduce systemic inflammation characteristic of chronic heart failure

Application of an Oil-Displacing Composition for Increasing Flow Rate of Low Producing High-Viscosity Oil Wells of the Usinskoye Oil Field

The results of a pilot application of a chemical composition for enhanced oil recovery developed at the IPC SB RAS are presented. The EOR-composition was tested in 2014 at the Permian-Carboniferous heavy oil deposit at the Usinskoye oil field. It is very effective for an increase in oil production rate and decrease in water cuttings of well production. In terms of the additionally produced oil, the resulting effect is up to 800 tons per well and its duration is up to 6 months. The application of technologies of low-productivity-well stimulation using the oil-displacing IKhNPRO system with controlled viscosity and alkalinity is thought to be promising. This composition is proposed for the ‘cold’ stimulation of high-viscosity oil production as an alternative to thermal methods