YY CMi: contact or near contact system?

New V photoelectric observations of the eclipsing system YY CMi, obtained at La Silla, Chile, and Merate Observatory, Italy, are presented. New times of minima and ephemeris based on our observations are also given. The V light curve was analysed by using the WD code to derive the geometrical and physical parameters of the system. Since no spectroscopic mass ratio is available, the q-search method was applied to yield the preliminary range of the mass ratio in order to search for the final solution. First the unspotted solution was carried out by using the unperturbed parts of the light curve and applying the DC program of the WD code. The solution was performed by assuming contact (mode 3) and semi-detached (mode 4) configuration, since no classification of the system is possible from the shape of the light curve. The solution in mode 4 does not lead to an acceptable model, since the secondary was found to be slightly overcontact. Therefore the contact solution was finally adopted. Moreover the light curve peculiarities (Max II fainter than Max I and excess of light around the phase 0.32) were explained by assuming a cool and a hot spot on the surface of the secondary (cooler) component. The degree of contact is very small (f about 3%) and the thermal contactis poor (T1-T2) about 650 K. These results together with the high photometric mass ratio q about 0.89 indicate that YY CMi is very probably a system at the beginning or the end of the contact phase.Comment: 7 pages, 7 ps figures. Accepted for Astronomy and Astrophysics, Supplement Serie

Frequency dependence of the dielectric and electro-optic response in suspensions of charged rod-like colloidal particles

We have performed an experimental investigation on the electrokinetic properties of charged rod-like fluorinated latex colloids. Systematic measurements of electrophoretic mobility, dielectric constant and electric birefringence have been performed as a function of the concentration of added nonionic surfactant and salt. In the investigated range of parameters, the zeta potential is a strongly decreasing function of the concentration of nonionic surfactant, while it is basically independent from ionic strength. We have obtained the frequency dependence of dielectric constant and Kerr constant as a function of zeta-potential and ionic strength. We observe the transition from a low frequency behavior, where both the dielectric constant and the Kerr constant are enhanced by the presence of the double layer, to a high frequency behavior, where both quantities take the value expected for unchanged particles in an insulating medium. The shape of the frequency dispersion of the Kerr constant coincides with that of the dielectric constant, but the cut-off frequencies are the same only when the zeta-potential of the particles is low

Characterization of anisotropic nano-particles by using depolarized dynamic light scattering in the near field

Light scattering techniques are widely used in many fields of condensed and sof t matter physics. Usually these methods are based on the study of the scattered light in the far field. Recently, a new family of near field detection schemes has been developed, mainly for the study of small angle light scattering. These techniques are based on the detection of the light intensity near to the sample, where light scattered at different directions overlaps but can be distinguished by Fourier transform analysis. Here we report for the first time data obtained with a dynamic near field scattering instrument, measuring both polarized and depolarized scattered light. Advantages of this procedure over the traditional far field detection include the immunity to stray light problems and the possibility to obtain a large number of statistical samples for many different wave vectors in a single instantaneous measurement. By using the proposed technique we have measured the translational and rotational diffusion coefficients of rod-like colloidal particles. The obtained data are in very good agreement with the data acquired with a traditional light scattering apparatus.Comment: Published in Optics Express. This version has changes in bibliograph

REM34 and REM35 control female and male gametophyte development in Arabidopsis thaliana

The REproductive Meristem (REM) gene family encodes for transcription factors belonging to the B3 DNA binding domain superfamily. In Arabidopsis thaliana the REM gene family is composed of 45 members, preferentially expressed during flower, ovule and seed development. Only a few members of this family have been functionally characterized: VERNALIZATION1 (VRN1) and most recently TARGET OF FLC AND SVP1 (TFS1) regulate flowering time and VERDANDI (VDD), together with VALKYRIE (VAL) control the death of the receptive synergid cell in the female gametophyte. We investigated the role of REM34, REM35 and REM36, three closely related and linked genes similarly expressed in both female and male gametophytes. Simultaneous silencing by RNA interference (RNAi) caused about 50% of the ovules to remain unfertilized. Careful evaluation of both ovule and pollen development showed that this partial sterility of the transgenic RNAi lines was due to a post meiotic block in both female and male gametophytes. Furthermore, protein interaction assays revealed that REM34 and REM35 interact, which suggests that they work together during the first stages of gametogenesis

Depicting conformational ensembles of \u3b1-synuclein by single molecule force spectroscopy and native mass spectroscopy

Description of heterogeneous molecular ensembles, such as intrinsically disordered proteins, represents a challenge in structural biology and an urgent question posed by biochemistry to interpret many physiologically important, regulatory mechanisms. Single-molecule techniques can provide a unique contribution to this field. This work applies single molecule force spectroscopy to probe conformational properties of \u3b1-synuclein in solution and its conformational changes induced by ligand binding. The goal is to compare data from such an approach with those obtained by native mass spectrometry. These two orthogonal, biophysical methods are found to deliver a complex picture, in which monomeric \u3b1-synuclein in solution spontaneously populates compact and partially compacted states, which are differently stabilized by binding to aggregation inhibitors, such as dopamine and epigallocatechin-3-gallate. Analyses by circular dichroism and Fourier-transform infrared spectroscopy show that these transitions do not involve formation of secondary structure. This comparative analysis provides support to structural interpretation of charge-state distributions obtained by native mass spectrometry and helps, in turn, defining the conformational components detected by single molecule force spectroscopy

Mean Curvature Flow on Ricci Solitons

We study monotonic quantities in the context of combined geometric flows. In particular, focusing on Ricci solitons as the ambient space, we consider solutions of the heat type equation integrated over embedded submanifolds evolving by mean curvature flow and we study their monotonicity properties. This is part of an ongoing project with Magni and Mantegazzawhich will treat the case of non-solitonic backgrounds \cite{a_14}.Comment: 19 page

Machine learning prediction models for mitral valve repairability and mitral regurgitation recurrence in patients undergoing surgical mitral valve repair

Background: Mitral valve regurgitation (MR) is the most common valvular heart disease and current variables associated with MR recurrence are still controversial. We aim to develop a machine learning-based prognostic model to predict causes of mitral valve (MV) repair failure and MR recurrence. Methods: 1000 patients who underwent MV repair at our institution between 2008 and 2018 were enrolled. Patients were followed longitudinally for up to three years. Clinical and echocardiographic data were included in the analysis. Endpoints were MV repair surgical failure with consequent MV replacement or moderate/severe MR (>2+) recurrence at one-month and mod-erate/severe MR recurrence after three years. Results: 817 patients (DS1) had an echocardiographic examination at one-month while 295 (DS2) also had one at three years. Data were randomly divided into training (DS1: n = 654; DS2: n = 206) and validation (DS1: n = 164; DS2 n = 89) cohorts. For intra-operative or early MV repair failure assessment, the best area under the curve (AUC) was 0.75 and the complexity of mitral valve prolapse was the main predictor. In predicting moderate/severe recurrent MR at three years, the best AUC was 0.92 and residual MR at six months was the most important predictor. Conclusions: Machine learning algorithms may improve prognosis after MV repair procedure, thus improving indications for correct candidate selection for MV surgical repair

Stellar Lyman-alpha Emission Lines in the Hubble Space Telescope Archive: Intrinsic Line Fluxes and Absorption from the Heliosphere and Astrospheres

We search the Hubble Space Telescope (HST) archive for previously unanalyzed observations of stellar H I Lyman-alpha emission lines, our primary purpose being to look for new detections of Lyman-alpha absorption from the outer heliosphere, and to also search for analogous absorption from the astrospheres surrounding the observed stars. The astrospheric absorption is of particular interest because it can be used to study solar-like stellar winds that are otherwise undetectable. We find and analyze 33 HST Lyman-alpha spectra in the archive. All the spectra were taken with the E140M grating of the Space Telescope Imaging Spectrograph (STIS) instrument on board HST. The HST/STIS spectra yield 4 new detections of heliospheric absorption (70 Oph, Xi Boo, 61 Vir, and HD 165185) and 7 new detections of astrospheric absorption (EV Lac, 70 Oph, Xi Boo, 61 Vir, Delta Eri, HD 128987, and DK UMa), doubling the previous number of heliospheric and astrospheric detections. When combined with previous results, 10 of 17 lines of sight within 10 pc yield detections of astrospheric absorption. This high detection fraction implies that most of the ISM within 10 pc must be at least partially neutral, since the presence of H I within the ISM surrounding the observed star is necessary for an astrospheric detection. In contrast, the detection percentage is only 9.7% (3 out of 31) for stars beyond 10 pc. Our Lyman-alpha analyses provide measurements of ISM H I and D I column densities for all 33 lines of sight, and we discuss some implications of these results. Finally, we measure chromospheric Lyman-alpha fluxes from the observed stars. We use these fluxes to determine how Lyman-alpha flux correlates with coronal X-ray and chromospheric Mg II emission, and we also study how Lyman-alpha emission depends on stellar rotation.Comment: 56 pages, 15 figures; AASTEX v5.0 plus EPSF extensions in mkfig.sty; accepted by ApJ

Surface Layer of Lactobacillus helveticus MIMLh5 Promotes Endocytosis by Dendritic Cells

Surface layers (S-layers) are proteinaceous arrays covering the cell walls of numerous bacteria. Their suggested properties, such as interactions with the host immune system, have been only poorly described. Here, we aimed to elucidate the role of the S-layer from the probiotic bacterial strain Lactobacillus helveticus MIMLh5 in the stimulation of murine bone-marrow-derived dendritic cells (DCs). MIMLh5 induced greater production of interferon beta (IFN-\u3b2), interleukin 10 (IL-10), and IL-12p70, compared to S-layer-depleted MIMLh5 (naked MIMLh5 [n-MIMLh5]), whereas the isolated S-layer was a poor immunostimulator. No differences in the production of tumor necrosis factor alpha (TNF-\u3b1) or IL-1\u3b2 were found. Inhibition of the mitogen-activated protein kinases JNK1/2, p38, and ERK1/2 modified IL-12p70 production similarly in MIMLh5 and n-MIMLh5, suggesting the induction of the same signaling pathways by the two bacterial preparations. Treatment of DCs with cytochalasin D to inhibit endocytosis before the addition of fluorescently labeled MIMLh5 cells led to a dramatic reduction in the proportion of fluorescence-positive DCs and decreased IL-12 production. Endocytosis and IL-12 production were only marginally affected by cytochalasin D pretreatment when fluorescently labeled n-MIMLh5 was used. Treatment of DCs with fluorescently labeled S-layer-coated polystyrene beads (Sl-beads) resulted in much greater uptake of beads, compared to noncoated beads. Prestimulation of DCs with cytochalasin D reduced the uptake of Sl-beads more than plain beads. These findings indicate that the S-layer plays a role in the endocytosis of MIMLh5 by DCs. In conclusion, this study provides evidence that the S-layer of L. helveticus MIMLh5 is involved in endocytosis of the bacterium, which is important for strong Th1-inducing cytokine production.IMPORTANCE Beneficial microbes may positively affect host physiology at various levels, e.g., by participating in immune system maturation and modulation, boosting defenses and dampening reactions, thus affecting the whole homeostasis. As a consequence, the use of probiotics is increasingly regarded as suitable for more extended applications for health maintenance, not only microbiota balancing. This implies a deep knowledge of the mechanisms and molecules involved in host-microbe interactions, for the final purpose of fine tuning the choice of a probiotic strain for a specific outcome. With this aim, studies targeted to the description of strain-related immunomodulatory effects and the identification of bacterial molecules responsible for specific responses are indispensable. This study provides new insights in the characterization of the food-origin probiotic bacterium L. helveticus MIMLh5 and its S-layer protein as a driver for the cross-talk with DCs