Pola Resistensi Acinetobacter Baumannii Yang Diisolasi Di Intensive Care Unit (Icu) RSUD Arifin Achmad Provinsi Riau Periode 1 Januari Hingga 31 Desember 2014

Bacterial resistance toward antibiotics occurs when antibiotics have lost their ability to effectively control or kill the growth of the bacteria. Bacterial resistance is a worldwide problem in both developing and developed countries. The purpose of this research is to know A. baumannii pattern and their antibiotics resistance isolated in an Intensive Care Unit of Arifin Achmad General Hospital in Riau Province period 1 January until 31 December 2014. This research used descriptive and retrospective design with the total number of isolates as much as 59 isolates. This study was carried out at Microbiology of the clinical Patologi Laboratory Arifin Achmad General Hospital in Riau Province using a Vitek Compact 2. The most commonly isolated bacteria of sputum culture were A. baumannii 34,7% meanwhile in blood culture A. baumannii was found as much as 1,7%. Resistance pattern showed that A. baumannii bacteria have the resistance against meropenem and the sensitivity were amikasin and tygesiclin

Nanoscale metal oxides 2D materials heterostructures for photoelectrochemical water splitting a review

Photoelectrochemical PEC water splitting is an interesting approach to harness clean and renewable solar energy to generate green hydrogen. To this end, metal oxides MOs have been investigated as photoelectrodes photoanode and photocathode due to their tunable optoelectronic properties and abundance, but achieving an efficient overall performance based on single MOs is very challenging due to their narrow visible light absorption, unfavorable band position, low charge mobilities, and limited stability. Heterostructuring MOs with other materials has therefore been proposed in the literature. In this review, we specifically highlight photoelectrodes based on the heterostructure of MOs and an emerging group of 2D materials consisting of mono elemental Xenes , carbides nitrides carbonitrides MXenes , boron carbon nitride BCNs , transition metal dichalcogenides TMDs , metal organic frameworks MOFs , and bismuth oxyhalides BiOX . The benefits of the formation of MOs 2D materials heterostructures are outlined, and the state of the art of MOs 2D materials heterostructures as photoelectrodes with various architectures are extensively discussed. Finally, a critical outlook on fundamental challenges and potential future directions in the development of MOs 2D materials heterostructures is presente

A simple method for generating full length cDNA from low abundance partial genomic clones

BACKGROUND: PCR amplification of target molecules involves sequence specific primers that flank the region to be amplified. While this technique is generally routine, its applicability may not be sufficient to generate a desired target molecule from two separate regions involving intron /exon boundaries. For these situations, the generation of full-length complementary DNAs from two partial genomic clones becomes necessary for the family of low abundance genes. RESULTS: The first approach we used for the isolation of full-length cDNA from two known genomic clones of Hox genes was based on fusion PCR. Here we describe a simple and efficient method of amplification for homeobox D13 (HOXD13) full length cDNA from two partial genomic clones. Specific 5' and 3' untranslated region (UTR) primer pairs and website program (primer3_www.cgv0.2) were key steps involved in this process. CONCLUSIONS: We have devised a simple, rapid and easy method for generating cDNA clone from genomic sequences. The full length HOXD13 clone (1.1 kb) generated with this technique was confirmed by sequence analysis. This simple approach can be utilized to generate full-length cDNA clones from available partial genomic sequences

The Helicobacter pylori Genome Project : insights into H. pylori population structure from analysis of a worldwide collection of complete genomes

Helicobacter pylori, a dominant member of the gastric microbiota, shares co-evolutionary history with humans. This has led to the development of genetically distinct H. pylori subpopulations associated with the geographic origin of the host and with differential gastric disease risk. Here, we provide insights into H. pylori population structure as a part of the Helicobacter pylori Genome Project (HpGP), a multi-disciplinary initiative aimed at elucidating H. pylori pathogenesis and identifying new therapeutic targets. We collected 1011 well-characterized clinical strains from 50 countries and generated high-quality genome sequences. We analysed core genome diversity and population structure of the HpGP dataset and 255 worldwide reference genomes to outline the ancestral contribution to Eurasian, African, and American populations. We found evidence of substantial contribution of population hpNorthAsia and subpopulation hspUral in Northern European H. pylori. The genomes of H. pylori isolated from northern and southern Indigenous Americans differed in that bacteria isolated in northern Indigenous communities were more similar to North Asian H. pylori while the southern had higher relatedness to hpEastAsia. Notably, we also found a highly clonal yet geographically dispersed North American subpopulation, which is negative for the cag pathogenicity island, and present in 7% of sequenced US genomes. We expect the HpGP dataset and the corresponding strains to become a major asset for H. pylori genomics

Voice Enable Personalized Web Search

The technology of voice browsing is rapidly evolving these days. It is because the use of cell phones is increasing at a very high rate, as compared to connected PCs. Speech interface integrated browser is a web browser that helps users by using an interactive voice user interface ,useful to those who have difficulties in seeing and reading a web content. Listening and speaking are the natural modes of communication and information gathering. As a result we are now heading towards a more voice based approach of browsing rather than operating on textual mode. A voice browser or speech browser will take and presents the information in the form of voice as well as text, using text to speech and speech to text conversion to render information. People want to get accurate and appropriate data at the top of search results in a user friendly manner. People also want to get a personal space over the internet when they are browsing on web, from this arises a need of personalization of the search history. Thus there is a need of a highly efficient and effective ranking algorithm that provides search results according to user preferences. This paper concentrates on this new technique, voice browsing, which unites speech recognition and speech synthesis with better personalized search that can be very fruitful in the coming years. In this paper we provide personalization by creating individual search history for each user on the browser and also focused on the search results to get customized according to the user demand

Thermal Annealing Effect on the Morphology of Inkjet Printed Polymer:Fullerene Composites Solar Cells

One of critical factor in determining the performance of solar cells is the morphology of the active layer. The drying process of the active layer prepared by inkjet printing technique plays a key role in obtaining high quality surface morphology. This paper reports the effect of thermal annealing on the morphology of printed active layer. The printed active layer is a blend of poly(3-octylthiophene-2,5-diyl) and (6,6)-phenyl $C_{71}$ butyric acid methyl ester dissolved in a mixture of dichlorobenzene:mesitylene. The printed films were then annealed at three different temperatures, namely 120, 140, and 160C for 60 min to obtain the best performance of solar cells. It was found that the performance of solar cells strongly depends on the annealing temperature. The devices with the active layer annealed at 140C exhibits the highest performance with short circuit current density and open circuit voltage as high as 2.88 μA/$cm^2$ and 0.85 V, respectively. The effect of annealing on the properties of printed active layer will be discussed