CHARACTERIZATION OF POLYHYDROXYBUTYRATE SYNTHESIZED BY BACILLUS CEREUS

Objectives: To characterize the Polyhydroxybutyrate (PHB) produced from Bacillus cereus using various instrumental methods and reduce them to the nanoscale which can be used as drug carrierMethods: The isolated bacterium was identified by 16S rDNA analysis. PHB produced by the bacterium was extracted by a process using boiling chloroform followed by methanol which selectively precipitates PHA. The polymer produced was analyzed using various instrumental techniques like FTIR, GC-MS, 1Hand 13C NMR, XRD and FESEM. This biogenic PHB has been reduced to nanospheres which were analyzed by FESEM.Results: A bacterium with the ability to produced PHB was isolated from the cloth used to smear oil on pan cake pan which was identified as Bacillus cereus by 16S rDNA analysis. The organism was capable of accumulating 1.19 g L-1 of PHA corresponding to 49.7% of its dry weight after 48 h of incubation. The polymer produced was analyzed using various instrumental techniques which identified the polymer as PHB.Conclusion: The organism seems to be a potential candidate for the biogenic synthesis of PHB which can find application as drug carrier.Â

Drug delivery system for chronic obstructive pulmonary diseases using IoT

Controlling drug delivery for ventilator circuits is the most difficult task. Most of the medicine is often lost in the exhaled breath, forcing the patient to breathe in a way that suits the flow of the medicine and can cause choking. The internal intercostal muscles contract during forced exhalation, lowering the chest and reducing chest volume, while the abdominal muscles push the diaphragm and contract the chest cavity. For multiple pulmonary lung disorders such as Chronic Obstructive Pulmonary Disease (COPD), asthma and others, this treatment has become an unavoidable part of the normal management strategy. Three types of inhalers that are often used include nebulizers, metered dosage inhalers, and dry powder inhalers. The most popular way to create medical aerosols is via spraying, which is done on both adults and children all over the world, particularly for at-home long-term lung disease therapy and emergency treatment of acute illnesses. The proposed system manages drug delivery in all ventilator environments with and without humidification and controls airflow for children, adults, and the elderly, and sends data to the appropriate people via IoT