表紙、目次、投稿規程、執筆要領、執筆者紹介

Currently a protein/peptide-mediated gene delivery has been considered a promising approach in non-viral gene transfer. The previous investigations have shown that histones and other nuclear proteins might be effective vectors for gene transfer into cells. Transfection of eukaryotic cells by nucleic acid and histone complexes (histonefection) effectively occurs with various histone proteins. The presence of DNA-binding domains and specific signal sequences of nuclear location allows to use histones (H1/H5, H2A, H2B, H3, H4) and other nuclear proteins (such as HMG family proteins and histonelike prokaryotic proteins) for recombinant genes transfer. The positive charge of histone protein molecules enables electrostatic interaction with negatively charged molecules of nucleic acids and charge neutralization that facilitates the complexes penetration through a negatively charged cell membrane. Thus, histonefection is a promising method for non-viral transfer of recombinant nucleic acids in gene therapy

Draft genome sequence of 'Cohnella kolymensis' B-2846

A draft genome sequence of "Cohnella kolymensis" strain B-2846 was derived using IonTorrent sequencing technology. The size of the assembly and G+C content were in agreement with those of other species of this genus. Characterization of the genome of a novel species of Cohnella will assist in bacterial systematics

МОДЕЛИ УПРАВЛЕНИЯ В СИСТЕМЕ УЧРЕЖДЕНИЙ СУДЕБНЫХ ЭКСПЕРТИЗ С ПОЗИЦИИ НАУЧНОГО МЕНЕДЖМЕНТА

The individual forensic activity in comparison with the activity of forensic experts of specialized state institutions is considered, the main advantages and disadvantages are determined. The directions of development of specialized state and non-state forensic institutions are presented in accordance with R. Quinn's competing values model.Розглянуто індивідуальну судово-експертну діяльність у порівнянні із діяльністю судових експертів спеціалізованих державних установ, наведено основні переваги та недоліки. Визначено напрями розвитку спеціалізованих державних і недержавних судово-експертних установ згідно з моделлю конкуруючих цінностей Р. Куінна

Spores of puffball fungus Lycoperdon pyriforme as a reference standard of stable monodisperse aerosol for calibration of optical instruments

Advanced air quality control requires real-time monitoring of particulate matter size and concentration, which can only be done using optical instruments. However, such techniques need regular calibration with reference samples. In this study, we suggest that puffball fungus (Lycoperdon pyriforme) spores can be utilized as a reference standard having a monodisperse size distribution. We compare the Lycoperdon pyriforme spores with the other commonly used reference samples, such as Al2O3 powder and polystyrene latex (PSL) microspheres. Here we demonstrate that the puffball spores do not coagulate and, thus, maintain the same particle size in the aerosol state for at least 15 minutes, which is enough for instrument calibration. Moreover, the puffball mushrooms can be stored for several years and no agglomeration of the spores occurs. They are also much cheaper than other calibration samples and no additional devices are needed for aerosol generation since the fungal fruiting body acts as an atomizer itself. The aforementioned features make the fungal spores a highly promising substance for calibration and validation of particle size analyzers, which outperforms the existing, artificially produced particles for aerosol sampling. Furthermore, the L. pyriforme spores are convenient for basic research and development of new optical measurement techniques, taking into account their uniform particle size and absent coagulation in the aeroso

Identification of electrofacies on the basis of well logging to determine sedimentation environment of horizon JK[2] in Em-Egovskoe field (Western Siberia)

Well logging results are one of the ways to study the buried terrigenous rocks genesis. To ensure the most objective characterization of the rock and identification of electrofacies it is necessary to use a complex geological and geophysical survey. The comprehensive investigations of environmental conditions based on well logging have been performed for the horizon JK[2] of Tumenskoe formation in Em-Egovskoe area, Krasnoleninskoe field (Western Siberia). The defined electrofacies were compared with the results of earlier conducted granulometric and mineralogical analyses. The totality of research provided for a conclusion that the investigated sediments of horizon JK2 had been formed within the destructive tidal delta. Thus, objective facies prediction can only be ensured by analyzing core and well logging data comprehensively