Evaluación biomédica de implantes dentales sometidos a cargas oblicuas: combinación de varias características geométricas

Objective: an efficient and simple methodology, based on virtual simulation and computer modelling, for the assessment and evaluation of dental implants design is proposed in this work. Materials and methods: the finite element method, a very common tool in engineering applications, is used for the evaluation the biomechanical performance of the biomedical devices. Von Mises stress is used as the main parameter to assess the suitability of the implant, when it is subjected to functional and biological loading. Also, this work reports the effects on the implant caused by geometrical variations such as length, diameter, thread, cortical bone thickness and abutment inclination. The useful-life of the implant was estimated by performing, in a virtual way, the fatigue tests required by the ISO:14801 standard. Results: for all the analyzed cases, maximum stress was obtained at the connecting screw under oblique loading. The estimated useful-life of the implant was around 5.000.000 cycles, which satisfies both ISO standard and functional requirements. Also, maximum stress was found in the compact-bone tissue surrounding the implant, which is in very good agreement with previous reports. Conclusion: a dental implant with optimal characteristics is proposed and validated using the discussed methodology.Peer ReviewedPostprint (published version

Equilibrium and non-equilibrium dynamics simultaneously operate in the Galápagos islands

Island biotas emerge from the interplay between colonisation, speciation and extinction and are often the scene of spectacular adaptive radiations. A common assumption is that insular diversity is at a dynamic equilibrium, but for remote islands, such as Hawaii or Galapagos, this idea remains untested. Here, we reconstruct the temporal accumulation of terrestrial bird species of the Galapagos using a novel phylogenetic method that estimates rates of biota assembly for an entire community. We show that species richness on the archipelago is in an ascending phase and does not tend towards equilibrium. The majority of the avifauna diversifies at a slow rate, without detectable ecological limits. However, Darwin's finches form an exception: they rapidly reach a carrying capacity and subsequently follow a coalescent-like diversification process. Together, these results suggest that avian diversity of remote islands is rising, and challenge the mutual exclusivity of the non-equilibrium and equilibrium ecological paradigms

On the interaction of the North Andes plate with the Caribbean and South American plates in northwestern South America from GPS geodesy and seismic data

We examine the hypocentral distribution of seismicity and a series of geodetic velocity vectors obtained from Global Positioning System observations between 1994 and 2015 both offshore and mainland northwestern South America (66°W–77°W; 8°N–14°N). Our analysis, that includes a kinematic block modelling, shows that east of the Caribbean–South American–North Andes plates’ triple junction at ∼68°W; 10.7°N, right-lateral easterly oriented shear motion (∼19.6 ± 2.0 mm yr−1) between the Caribbean and South America plates is split along two easterly striking, right-lateral strike-slip subparallel fault zones: the San Sebastián fault that runs off-shore the Venezuelan coast and slips about 17.0±0.5mm yr−1 and the La Victoria fault, located on-shore to the south, which is accumulating strain equivalent to 2.6 ± 0.4 mm yr−1.West of the triple junction, relative right-lateral motion between the Caribbean and South American plates is mostly divided between the Morrocoy and Boconó fault systems that strike northwest and southwest from the triple junction, respectively, and bound the intervening North Andes plate that shows an easterly oriented geodetic slip of 15.0 ± 1.0 mm yr−1 relative to the South American plate. Slip on the Morrocoy fault is right-lateral and transtensional. Motion across the Boconó fault is also right-lateral but instead transpressional, divided between ∼9 and 11 mm yr−1 of right-slip on the Bocon´o fault and 2–5 mm yr−1 of convergence across adjacent and subparallel thrust faults. Farther west of the triple junction, ∼800 km away in northern Colombia, the Caribbean plate subducts to the southeast beneath the North Andes plate at a geodetically estimated rate of ∼5–7 mm yr−1

Novel flaviviruses from mosquitoes: Mosquito-specific evolutionary lineages within the phylogenetic group of mosquito-borne flaviviruses

Copyright © 2014 The Authors. Published by Elsevier Inc. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The attached file is the published version of the article

Pentamidine Is Not a Permeant but a Nanomolar Inhibitor of the Trypanosoma brucei Aquaglyceroporin-2

The chemotherapeutic arsenal against human African trypanosomiasis, sleeping sickness, is limited and can cause severe, often fatal, side effects. One of the classic and most widely used drugs is pentamidine, an aromatic diamidine compound introduced in the 1940s. Recently, a genome-wide loss-of-function screen and a subsequently generated trypanosome knockout strain revealed a specific aquaglyceroporin, TbAQP2, to be required for high-affinity uptake of pentamidine. Yet, the underlying mechanism remained unclear. Here, we show that TbAQP2 is not a direct transporter for the di-basic, positively charged pentamidine. Even though one of the two common cation filters of aquaglyceroporins, i.e. the aromatic/arginine selectivity filter, is unconventional in TbAQP2, positively charged compounds are still excluded from passing the channel. We found, instead, that the unique selectivity filter layout renders pentamidine a nanomolar inhibitor of TbAQP2 glycerol permeability. Full, non-covalent inhibition of an aqua(glycero)porin in the nanomolar range has not been achieved before. The remarkable affinity derives from an electrostatic interaction with Asp265 and shielding from water as shown by structure-function evaluation and point mutation of Asp265. Exchange of the preceding Leu264 to arginine abolished pentamidine-binding and parasites expressing this mutant were pentamidine-resistant. Our results indicate that TbAQP2 is a high-affinity receptor for pentamidine. Taken together with localization of TbAQP2 in the flagellar pocket of bloodstream trypanosomes, we propose that pentamidine uptake is by endocytosis

110 Years of Avipoxvirus in the Galapagos Islands

The role of disease in regulating populations is controversial, partly owing to the absence of good disease records in historic wildlife populations. We examined birds collected in the Galapagos Islands between 1891 and 1906 that are currently held at the California Academy of Sciences and the Zoologisches Staatssammlung Muenchen, including 3973 specimens representing species from two well-studied families of endemic passerine birds: finches and mockingbirds. Beginning with samples collected in 1899, we observed cutaneous lesions consistent with Avipoxvirus on 226 (6.3%) specimens. Histopathology and viral genotyping of 59 candidate tissue samples from six islands showed that 21 (35.6%) were positive for Avipoxvirus, while alternative diagnoses for some of those testing negative by both methods were feather follicle cysts, non-specific dermatitis, or post mortem fungal colonization. Positive specimens were significantly nonrandomly distributed among islands both for mockingbirds (San Cristobal vs. Espanola, Santa Fe and Santa Cruz) and for finches (San Cristobal and Isabela vs. Santa Cruz and Floreana), and overall highly significantly distributed toward islands that were inhabited by humans (San Cristobal, Isabela, Floreana) vs. uninhabited at the time of collection (Santa Cruz, Santa Fe, Espanola), with only one positive individual on an uninhabited island. Eleven of the positive specimens sequenced successfully were identical at four diagnostic sites to the two canarypox variants previously described in contemporary Galapagos passerines. We conclude that this virus was introduced late in 1890′s and was dispersed among islands by a variety of mechanisms, including regular human movements among colonized islands. At present, this disease represents an ongoing threat to the birds on the Galapagos Islands