Localization of dexamethasone within dendritic core-multishell (CMS) nanoparticles and skin penetration properties studied by multi-frequency electron paramagnetic resonance (EPR) spectroscopy

The skin and especially the stratum corneum (SC) act as a barrier and protect epidermal cells and thus the whole body against xenobiotica of the external environment. Topical skin treatment requires an efficient drug delivery system (DDS). Polymer-based nanocarriers represent novel transport vehicles for dermal application of drugs. In this study dendritic core-multishell (CMS) nanoparticles were investigated as promising candidates. CMS nanoparticles were loaded with a drug (analogue) and were applied to penetration studies of skin. We determined by dual-frequency electron paramagnetic resonance (EPR) how dexamethasone (Dx) labelled with 3-carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy (PCA) is associated with the CMS. The micro-environment of the drug loaded to CMS nanoparticles was investigated by pulsed high-field EPR at cryogenic temperature, making use of the fact that magnetic parameters (g-, A-matrices, and spin-lattice relaxation time) represent specific probes for the micro-environment. Additionally, the rotational correlation time of spin-labelled Dx was probed by continuous wave EPR at ambient temperature, which provides independent information on the drug environment. Furthermore, the penetration depth of Dx into the stratum corneum of porcine skin after different topical applications was investigated. The location of Dx in the CMS nanoparticles is revealed and the function of CMS as penetration enhancers for topical application is shown

Bridging the Semantic Web and NoSQL Worlds: Generic SPARQL Query Translation and Application to MongoDB

International audienceRDF-based data integration is often hampered by the lack of methods to translate data locked in heterogeneous silos into RDF representations. In this paper, we tackle the challenge of bridging the gap between the Semantic Web and NoSQL worlds, by fostering the development of SPARQL interfaces to heterogeneous databases. To avoid defining yet another SPARQL translation method for each and every database, we propose a two-phase method. Firstly, a SPARQL query is translated into a pivot abstract query. This phase achieves as much of the translation process as possible regardless of the database. We show how optimizations at this abstract level can save subsequent work at the level of a target database query language. Secondly, the abstract query is translated into the query language of a target database, taking into account the specific database capabilities and constraints. We demonstrate the effectiveness of our method with the MongoDB NoSQL document store, such that arbitrary MongoDB documents can be aligned on existing domain ontologies and accessed with SPARQL. Finally, we draw on a real-world use case to report experimental results with respect to the effectiveness and performance of our approach

Robust control of microvibrations with experimental verification

The paper addresses the problem of actively attenuating a particular class of vibrations, known as microvibrations, which arise, for example, in panels used on satellites. A control scheme which incorporates feedback action is developed which operates at a set of dominant frequencies in a disturbance spectrum, where the control path model is estimated online. Relative to earlier published techniques, a new feature of the presented controller is the use of the inverse Hessian to improve adaptation speed. The control scheme also incorporates a frequency estimation technique to determine the relevant disturbance frequencies with higher precision than the standard fast Fourier transform (FFT). The control scheme is implemented on an experimental test-bed and the total achieved attenuation, as measured from the experiments, is 26dB. The low computational demand of the control scheme allows for single chip controller implementation, a feature which is particularly attractive for potential applications areas, such as small satellites, where there are critical overall weight restrictions to be satisfied whilst delivering high quality overall performance