Making Prevention Work: Case Study Netherlands. Materials about Prevention Vol. 18 June 2020

As part of a larger project mapping preventive structures and policies for children, young people and families in 12 European countries, the Making Prevention Work study aims to provide a consistent base for developing preventive policies in Europe. It examines approaches across the EU that demonstrate success with local preventive work. The in-depth case study of the Netherlands presented in this publication is one of three published in the context of the Making Prevention Work study. Making Prevention Work draws on a concept of prevention that is framed in universalist and integrative terms. The concept is universalist in that it addresses all children and young people, even those not seen as being “at-risk.” It is integrative because prevention should be organized from a child’s point of view, not in terms of administrative responsibilities. As such, this concept targets the establishment of prevention chains that link different institutions over the life-course. Making Prevention Work includes summary factsheets of the preventive concepts, structures and practices mapped in 12 EU member states (Austria, Czech Republic, Denmark, England (UK), Finland, France, Germany, Ireland, Lithuania, the Netherlands, Spain and Sweden) as well as three case studies (Austria, France and the Netherlands) featuring data from interviews with experts and implementing actors

Making Prevention Work: Preventive structures and policies for children, youth and families: Comprehensive report. Materials about Prevention Vol. 15 June 2020.

This report maps preventive structures and policies for children, young people and families in 12 European countries. By examining what works in each of the countries surveyed, it aims to provide a foundation for the development of prevention policies across Europe. The report draws on a concept of prevention that is framed in universalist and integrative terms. The concept is universalist in that it addresses all children and young people, even those not seen as being “at-risk.” It is integrative because prevention should be organized from a child’s point of view, not in terms of administrative responsibilities. As such, this concept targets the establishment of prevention chains that link different institutions over the life-course. The report includes summary factsheets of the preventive concepts, structures and practices mapped in 12 EU member states (Austria, Czechia, Denmark, England (UK), Finland, France, Germany, Ireland, Lithuania, the Netherlands, Spain and Sweden). In addition, three in-depth case studies (Austria, France and the Netherlands) featuring data from interviews with experts and implementing actors are also presented

The Influence of Text on Coherence of Story Retells, Reading Comprehension, Vocabulary Acquisition, and Eye Gaze: A Computer-Based Story Telling Task with Eye Tracking

With the growing trend of using multimedia platforms such as YouTube to facilitate storytelling, understanding how and when to integrate text with visuals would benefit both the creators of these platforms and the young readers viewing them. The current study examined the effect of orthography on vocabulary acquisition and narrative comprehension in young readers (children in 2nd and 3rd grade, ages 6-9) during a computer-based storytelling task. We aimed to determine if having text available during storytelling benefits readers as predicted by Perfetti’s Lexical Quality Hypothesis (Perfetti & Hart, 2002), or hampers learning as predicted by Mayer’s Redundancy Effect (Moreno & Mayer, 2002). The dissertation also explored if having access to the text would affect children’s eye gaze patterns while viewing the story, which had been found during the pilot study. It was hypothesized that the group who had access to their native English text would perform better in measures of comprehension and vocabulary knowledge and that they would show a different eye gaze pattern during the computer-based storytelling task than the group presented with Greek symbols in place of English. Children completed a battery of pretests to assess reading level and working memory ability and then were randomly assigned to conditions where picture books displayed either the story text in English (meaningful condition) or in Greek symbols (meaningless text condition). They then watched a PowerPoint presentation featuring Mercer Mayer’s “Frog, where are you?” (Mayer, 1969) while having their eye gaze monitored using Tobii 4C eye tracker. After viewing the presentation, the children retold the story and answered ten comprehension questions, assessing both direct and inferential comprehension. They then completed a vocabulary knowledge scale that used words from the stories to assess vocabulary comprehension, as well as a cloze task created by the experimenter using target words from the story. Results found that the presence of text did not affect comprehension or vocabulary acquisition, as both groups had similar scores across measures. However, the study found significant differences in eye gaze patterns between the groups. In both conditions participants spent significantly more time viewing the text during the beginning of the story and then reduced their dwell time during the middle and the end. Participants in both conditions also spent a higher percentage of dwell time on the illustrations during the beginning and middle of the story before reducing their focus at the end. However, when compared to participants exposed to the Greek symbols, participants with access to their native text spent significantly less time viewing the story pictures overall. Results also found that for each page of the story, all participants focused on the text more before and during the playing of the recorded audio narration than after the narration was completed. An interaction between condition and the time stage of narration on dwell time suggested that narration affected participants differently depending on whether they had access to the meaningful text. Specifically, the participants with access to their native English text showed variation in their dwell times between the three different time stages: before, during, and after narration, which was not seen in the group who saw the Greek symbols. While the study needs to be replicated to confirm results due to limitations caused by sample size and data loss in eye tracking, the present findings could help educators better integrate multimedia story telling within their classrooms by designing the activities to better match their students’ attentional and cognitive needs

Genetics, sleep and memory:a recall-by-genotype study of ZNF804A variants and sleep neurophysiology

© 2015 Hellmich et al.Background: Schizophrenia is a complex, polygenic disorder for which over 100 genetic variants have been identified that correlate with diagnosis. However, the biological mechanisms underpinning the different symptom clusters remain undefined. The rs1344706 single nucleotide polymorphism within ZNF804A was among the first genetic variants found to be associated with schizophrenia. Previously, neuroimaging and cognitive studies have revealed several associations between rs1344706 and brain structure and function. The aim of this study is to use a recall-by-genotype (RBG) design to investigate the biological basis for the association of ZNF804A variants with schizophrenia. A RBG study, implemented in a population cohort, will be used to evaluate the impact of genetic variation at rs1344706 on sleep neurophysiology and procedural memory consolidation in healthy participants. Methods/Design: Participants will be recruited from the Avon Longitudinal Study of Parents and Children (ALSPAC) on the basis of genotype at rs1344706 (n = 24). Each participant will be asked to take part in two nights of in-depth sleep monitoring (polysomnography) allowing collection of neurophysiological sleep data in a manner not amenable to large-scale study. Sleep questionnaires will be used to assess general sleep quality and subjective sleep experience after each in-house recording. A motor sequencing task (MST) will be performed before and after the second night of polysomnography. In order to gather additional data about habitual sleep behaviour participants will be asked to wear a wrist worn activity monitor (actiwatch) and complete a sleep diary for two weeks. Discussion: This study will explore the biological function of ZNF804A genotype (rs1344706) in healthy volunteers by examining detailed features of sleep architecture and physiology in relation to motor learning. Using a RBG approach will enable us to collect precise and detailed phenotypic data whilst achieving an informative biological gradient. It would not be feasible to collect such data in the large sample sizes that would be required under a random sampling scheme. By dissecting the role of individual variants associated with schizophrenia in this way, we can begin to unravel the complex genetic mechanisms of psychiatric disorders and pave the way for future development of novel therapeutic approaches

Translational neurophysiology in sheep:Measuring sleep and neurological dysfunction in CLN5 affected Batten disease sheep

This is the final published version of a paper originally published in BRAIN 2015: 138; 862?874, DOI: http://dx.doi.org/10.1093/brain/awv026Creating valid mouse models of slowly progressing human neurological diseases is challenging, not least because the short lifespan of rodents confounds realistic modelling of disease time course. With their large brains and long lives, sheep offer significant advantages for translational studies of human disease. Here we used normal and CLN5 Batten disease affected sheep to demonstrate the use of the species for studying neurological function in a model of human disease. We show that electroencephalography can be used in sheep, and that longitudinal recordings spanning many months are possible. This is the first time such an electroencephalography study has been performed in sheep. We characterized sleep in sheep, quantifying characteristic vigilance states and neurophysiological hallmarks such as sleep spindles. Mild sleep abnormalities and abnormal epileptiform waveforms were found in the electroencephalographies of Batten disease affected sheep. These abnormalities resemble the epileptiform activity seen in children with Batten disease and demonstrate the translational relevance of both the technique and the model. Given that both spontaneous and engineered sheep models of human neurodegenerative diseases already exist, sheep constitute a powerful species in which longitudinal in vivo studies can be conducted. This will advance our understanding of normal brain function and improve our capacity for translational research into neurological disorders.This work was funded by CHDI Inc. (AJM). Founding the\ud sheep flock, and costs in NZ relating to the rearing and\ud genotyping of the animals were funded by a series of grants\ud from the Neurological Foundation of NZ and the Batten\ud Disease Support and Research Association (DNP, NLM)

Schizophrenia-associated variation at <i>ZNF804A</i> correlates with altered experience-dependent dynamics of sleep slow waves and spindles in healthy young adults

The rs1344706 polymorphism in ZNF804A is robustly associated with schizophrenia and schizophrenia is, in turn, associated with abnormal non-rapid eye movement (NREM) sleep neurophysiology. To examine whether rs1344706 is associated with intermediate neurophysiological traits in the absence of disease, we assessed the relationship between genotype, sleep neurophysiology, and sleep-dependent memory consolidation in healthy participants. We recruited healthy adult males with no history of psychiatric disorder from the Avon Longitudinal Study of Parents and Children (ALSPAC) birth cohort. Participants were homozygous for either the schizophrenia-associated 'A' allele (N = 22) or the alternative 'C' allele (N = 18) at rs1344706. Actigraphy, polysomnography (PSG) and a motor sequence task (MST) were used to characterize daily activity patterns, sleep neurophysiology and sleep-dependent memory consolidation. Average MST learning and sleep-dependent performance improvements were similar across genotype groups, albeit more variable in the AA group. During sleep after learning, CC participants showed increased slow-wave (SW) and spindle amplitudes, plus augmented coupling of SW activity across recording electrodes. SW and spindles in those with the AA genotype were insensitive to learning, whilst SW coherence decreased following MST training. Accordingly, NREM neurophysiology robustly predicted the degree of overnight motor memory consolidation in CC carriers, but not in AA carriers. We describe evidence that rs1344706 polymorphism in ZNF804A is associated with changes in the coordinated neural network activity that supports offline information processing during sleep in a healthy population. These findings highlight the utility of sleep neurophysiology in mapping the impacts of schizophrenia-associated common genetic variants on neural circuit oscillations and function

The Spectral Ocean Color Imager (SPOC) – An Adjustable Multispectral Imager

SPOC (SPectral Ocean Color) is a 3U small satellite mission that will use an adjustable multispectral imager to map sensitive coastal regions and off coast water quality of Georgia and beyond. SPOC is being developed by the University of Georgia’s (UGA) Small Satellite Research Laboratory (SSRL) through NASA’s Undergraduate Student Instrument Project (USIP). UGA is working with Cloudland Instruments to develop a small scale (\u3c 1000 \u3ecm3) multispectral imager, ranging from 400-850nm, for Earth science applications which will fly as part of the NASA CubeSat Launch Initiative. The project is UGA’s first satellite mission and is built by a team of undergraduates from a wide range of backgrounds and supervised by a multidisciplinary team of graduate students and faculty. Development, assembly, testing, and validation of the multispectral imager, as well integrating it into the satellite are all being done in house. At an orbit of 400 km the resulting images will be 90 km x 100 km in size, with a default spatial resolution and spectral resolution of 130 m and 4 nm, respectively

Sleep EEG in young people with 22q11.2 deletion syndrome:a cross-sectional study of slow-waves, spindles and correlations with memory and neurodevelopmental symptoms

Background:: Young people living with 22q11.2 Deletion Syndrome (22q11.2DS) are at increased risk of schizophrenia, intellectual disability, attention-deficit hyperactivity disorder (ADHD) and autism spectrum disorder (ASD). In common with these conditions, 22q11.2DS is also associated with sleep problems. We investigated whether abnormal sleep or sleep-dependent network activity in 22q11.2DS reflects convergent, early signatures of neural circuit disruption also evident in associated neurodevelopmental conditions. Methods:: In a cross-sectional design, we recorded high-density sleep EEG in young people (6–20 years) with 22q11.2DS (n=28) and their unaffected siblings (n=17), quantifying associations between sleep architecture, EEG oscillations (spindles and slow waves) and psychiatric symptoms. We also measured performance on a memory task before and after sleep. Results:: 22q11.2DS was associated with significant alterations in sleep architecture, including a greater proportion of N3 sleep and lower proportions of N1 and REM sleep than in siblings. During sleep, deletion carriers showed broadband increases in EEG power with increased slow-wave and spindle amplitudes, increased spindle frequency and density, and stronger coupling between spindles and slow-waves. Spindle and slow-wave amplitudes correlated positively with overnight memory in controls, but negatively in 22q11.2DS. Mediation analyses indicated that genotype effects on anxiety, ADHD and ASD were partially mediated by sleep EEG measures. Conclusions:: This study provides a detailed description of sleep neurophysiology in 22q11.2DS, highlighting alterations in EEG signatures of sleep which have been previously linked to neurodevelopment, some of which were associated with psychiatric symptoms. Sleep EEG features may therefore reflect delayed or compromised neurodevelopmental processes in 22q11.2DS, which could inform our understanding of the neurobiology of this condition and be biomarkers for neuropsychiatric disorders. Funding:: This research was funded by a Lilly Innovation Fellowship Award (UB), the National Institute of Mental Health (NIMH 5UO1MH101724; MvdB), a Wellcome Trust Institutional Strategic Support Fund (ISSF) award (MvdB), the Waterloo Foundation (918-1234; MvdB), the Baily Thomas Charitable Fund (2315/1; MvdB), MRC grant Intellectual Disability and Mental Health: Assessing Genomic Impact on Neurodevelopment (IMAGINE) (MR/L011166/1; JH, MvdB and MO), MRC grant Intellectual Disability and Mental Health: Assessing Genomic Impact on Neurodevelopment 2 (IMAGINE-2) (MR/T033045/1; MvdB, JH and MO); Wellcome Trust Strategic Award ‘Defining Endophenotypes From Integrated Neurosciences’ Wellcome Trust (100202/Z/12/Z MO, JH). NAD was supported by a National Institute for Health Research Academic Clinical Fellowship in Mental Health and MWJ by a Wellcome Trust Senior Research Fellowship in Basic Biomedical Science (202810/Z/16/Z). CE and HAM were supported by Medical Research Council Doctoral Training Grants (C.B.E. 1644194, H.A.M MR/K501347/1). HMM and UB were employed by Eli Lilly & Co during the study; HMM is currently an employee of Boehringer Ingelheim Pharma GmbH & Co KG. The views and opinions expressed are those of the author(s), and not necessarily those of the NHS, the NIHR or the Department of Health funders