Antioxidant potential and modulatory effects of restructured lipids from the amazonian palms on liver cells

Enzymatic interesterification is used to manipulate oil and fat in order to obtain improved restructured lipids with desired technological properties. However, with raw materials containing significant amounts of bioactive compounds, the influence of this enzymatic process on the bioactivity of the final product is still not clear. Thus, the aim of this study is to evaluate the antioxidant potential and modulatory effects of two raw materials from the Amazonian area, buriti oil and murumuru fat, before and after lipase interesterification, on human hepatoma cells (HepG2). The results indicate that minor bioactive compounds naturally found in the raw materials and their antioxidant capacity are preserved after enzymatic interesterification, and that the restructured lipids modulate HepG2 endogenous antioxidant enzyme554553561CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP2012- -22774-5; 2012-22829-4Sem informaçãoSem informaçã

Antioxidant Potential and Modulatory Effects of Restructured Lipids from the Amazonian Palms on Liver Cells

Enzimska interesterifikacija koristi se za obradu ulja i masti radi dobivanja restrukturiranih lipida sa željenim tehnološkim svojstvima. Međutim, s obzirom na to da sirovi materijali sadržavaju velike udjele bioaktivnih tvari, još uvijek je nejasan utjecaj ovakvih enzimskih procesa na biološku aktivnost konačnog produkta. Stoga je svrha ovog istraživanja bila procijeniti antioksidacijski potencijal i modulacijski učinak dvaju sirovih materijala iz područja Amazone (buriti ulja i murumuru masti) na stanice jetre (HepG2), i to prije i nakon procesa interesterifikacije. Istraživanje je pokazalo da su neke bioaktivne komponente sirovih materijala i njihov antioksidacijski učinak očuvani nakon enzimske esterifikacije restrukturiranih lipida, pri čemu dolazi do modulacije HepG2 endogenog antioksidacijskog enzima.Enzymatic interesterification is used to manipulate oil and fat in order to obtain improved restructured lipids with desired technological properties. However, with raw materials containing significant amounts of bioactive compounds, the influence of this enzymatic process on the bioactivity of the final product is still not clear. Thus, the aim of this study is to evaluate the antioxidant potential and modulatory effects of two raw materials from the Amazonian area, buriti oil and murumuru fat, before and after lipase interesterification, on human hepatoma cells (HepG2). The results indicate that minor bioactive compounds naturally found in the raw materials and their antioxidant capacity are preserved after enzymatic interesterification, and that the restructured lipids modulate HepG2 endogenous antioxidant enzyme

“Mangá caboclo” do Brasil: Transformações da cultura pop japonesa na convergência digital

O presente artigo trata da reprodução e consumo de mangá (quadrinhos japoneses) no Brasil. O recorte de pesquisa são os quadrinhos brasileiros Turma da Mônica Jovem, em estilo mangá, e o modelo de negócios para o segmento que o produto editorial inaugura no país a partir da convergência com novos meios digitais. O Brasil desenvolveu historicamente uma relação singular com o mangá, que influenciou a produção brasileira de quadrinhos e demandou o desenvolvimento de um modelo de marketing editorial atento às transformações culturais e marcado pela convergência midiática na vida contemporânea.  This article is about reproduction and consumption of manga (Japanese comics) in Brazil. The research outline is the Brazilian comic book Turma da Mônica Jovem (Monica Teen), in manga style, and the business model for the segment this editorial product has launched in that country starting with the convergence of new digital media. Brazil has historically developed a unique relationship with manga, which influenced the Brazilian production of comics and demanded the development of an editorial marketing model aware of cultural changes and marked by media convergence in contemporary life.Este artículo es sobre la reproducción y consumo de mangá (historietas japonesas) en Brasil. La sección de esta investigación es las historietas brasileñas llamadas Turma da Mônica Jovem, en estilo mangá, y el modelo de negocios para el segmento que este producto editorial estrena en el paós desde la convergencia con nuevos medios digitales. Brasil ha desarrollado historicamente una relacción singular con el mangá, que ha influenciado la producción local de historietas y exigió el desarrollo de un modelo de marketing editorial listo para las transformaciones culturales y marcado por la convergencia de medios en la vida contemporánea

Exploring in vitro effects of biotransformed isoflavones extracts : antioxidant, antiinflammatory, and antilipogenic

The present study aimed to investigate, in in vitro assays, the antilipogenic and antiinflammatory potential as well as the antioxidant capacity of biotransformed soymilk by tannase and beta-glycosidase enzymes. The results showed a significant enhancement of the antioxidant capacity, especially by biotransformed soymilk with free tannase (SFT), corresponding to an increase of 2.3 and 1.25 times by oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays, respectively. The lipid accumulation reduction by 3T3-L1 adipocytes assay was not significant. However, the antiinflammatory responses were expressive. In lipopolysaccharide-stimulated RAW 264.7 macrophages, SFT reduced around 37 times TNF-alpha expression at the highest tested concentration of the sample. Other inflammatory parameters, as IL-6 and nitric oxide, were no longer detected when the cells were treated with SFT and soymilk with immobilized enzymes, respectively. The biotransformed soy extracts with tannase have great potential to act as a nutraceutical, protecting the cells against oxidative damage and helping maintain health under inflammatory stress. Practical applications Soy isoflavones have been associated with several beneficial effects on human health, including inhibition capacity of lipid accumulation in adipocytes, antiinflammatory properties, and antioxidant potential. However, the isoflavones bioavailability differs among their chemical forms, and studies have shown that the higher health benefits are conferred by aglycones and their metabolites, such as equol, compared to the other forms. For this reason, the enrichment of isoflavone aglycones and metabolites in soy-based products has attracted growing attention. The present study was focused on developing a bioprocess able to produce a rich extract with soy isoflavones metabolites, with increased bioactive potential for application as a functional ingredient or a nutraceutical437COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPnão tem17367-

Enhanced estrogenic effects of biotransformed soy extracts

Soy isoflavones have been associated with several beneficial effects to human health including estrogenic action. Due to the risks associated with pharmaceutical hormone replacement therapy, soy extracts rich in isoflavones and metabolites appear as a safe alternative for menopausal women. This study aimed to improve the process conditions for biotransformation of soymilk polyphenols by tannase to obtain higher amounts of isoflavone aglycones and metabolites such as equol. The estrogenic potential of the extracts was evaluated in in vitro assays. The results showed that the aglycones increased 36–46 times after reaction with tannase and proved that it is possible to produce equol in soymilk using an enzymatic bioprocess, without gut microbial intervention. Moreover, the soymilk biotransformed by tannase presented higher estrogenic action in the MCF-7 BUS cell line assay, making it a promising nutraceutical with possible effects in the attenuation or treatment of menopause symptoms48117124COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informação17367-7, 201

beta(1) Adrenergic receptor is key to cold- and diet-induced thermogenesis in mice

Brown adipose tissue (BAT) is predominantly regulated by the sympathetic nervous system (SNS) and the adrenergic receptor signaling pathway. Knowing that a mouse with triple beta-receptor knockout (KO) is cold intolerant and obese, we evaluated the independent role played by the beta(1) isoform in energy homeostasis. First, the 30 min i.v. infusion of norepinephrine (NE) or the beta(1) selective agonist dobutamine (DB) resulted in similar interscapular BAT (iBAT) thermal response in WT mice. Secondly, mice with targeted disruption of the beta(1) gene (KO of beta(1) adrenergic receptor (beta 1KO)) developed hypothermia during cold exposure and exhibited decreased iBAT thermal response to NE or DB infusion. Thirdly, when placed on a high-fat diet (HFD; 40% fat) for 5 weeks, beta 1KO mice were more susceptible to obesity than WT controls and failed to develop diet-induced thermogenesis as assessed by BAT Ucp1 mRNA levels and oxygen consumption. Furthermore, beta 1KO mice exhibited fasting hyperglycemia and more intense glucose intolerance, hypercholesterolemia, and hypertriglyceridemia when placed on the HFD, developing marked non-alcoholic steatohepatitis. In conclusion, the beta(1) signaling pathway mediates most of the SNS stimulation of adaptive thermogenesis. Journal of Endocrinology (2012) 214, 359-365Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)MackPesquisa, BrazilMackPesquisa, Brazi