In vitro effects of Musa x paradisiaca extracts on four developmental stages of Haemonchus contortus

International audienceThis study was carried out to evaluate the in vitro effect of Musa x paradisiaca stem and leaf against the parasitic nematode of small ruminants Haemonchus contortus. Three extracts (aqueous, methanolic and/or dichloromethane) of Musa x paradisiaca stem and leaf were tested in vitro on four developmental stages of H. contortus using egg hatch assay (ERA), larval development assay (LDA), 13 migration inhibition assay (LMI) and adult worm motility assay (AWM). The highly significant (P 67% for each extract) and the negative effect of the dichloromethane extract of leaf on adult worm motility (43% of inhibition of motility after 24 h of incubation) compared to the negative controls, suggest anthelmintic properties of Musa x paradisiaca stem and leaf against H. contortus. The active principles responsible for the activity could be secondary metabolites such as terpenoid and flavonoid compounds present in the leaf and stem of the plant

Various condensed tannins from tropical plants as potential multi-purpose nutraceutic in ruminant feed

Session 9: Nutritional management of health and welfareNational audienc

In vitro effects of Cassava (Manihot esculenta) leaf extracts on four development stages of Haemonchus contortus

International audienceThree extracts (aqueous, methanolic and dichloromethane) of Manihot esculenta (Cassava) leaf were tested in vitro on four development stages of Haemonchus contortus using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition assay (LMI) and adult worm motility assay (AWM). Compared to the negative control, significant effects (P < 0.0001) were observed for the methanolic extract of leaf against larval development (57.6% ±7.6), with a dose dependent effect. These results suggest that Cassava possess anthelmintic activity against H. contortus. The active principles responsible for the activity could be the terpenoids and condensed tannin compounds present in the leaf

Various condensed tannins from tropical plants. Potential multipurpose nutraceutics in ruminant feed

Condensed tannins (CT) are bio-reactive complex plant secondary metabolites. Protein-CT complexes could result in affection of rumen fermentation, allow availability of amino-acids and induce a gastrointestinal nematicidal action in ruminants. In this study 5 tropical plants were evaluated to provide some natural multi-active nutraceutics to animal production. The CT composition of plants was quantified and elucidated using vanillin-H2S04 assay and LC-MS analysis. CT extracts were assayed for bioactivity against the exsheathment of the nematode Haemonchus contortus, and fermentation profiles of plants (CH4 and NH3-N produced, organic matter digestibility (OMD)) were determined in vitro. Thiolysis analysis showed that CT extracts contained from 1.7 to 59 g of CT per 100g. mDP ranged from 3.3 to 15.3, PC/PD ratios from 0 to 10.7, cis/trans flavan-3-ol ratios from 1.7 to 24.7, and galloyl groups from 0 to 37.4 %. CT had a high effect (p<0.001) on nematicidal activity and fermentation profile.The complexity of composition of CT in tropical plants and their multipurpose potential for animal production were highlighted. It seems that CT chemical composition may influence bio-reactivity and neutracutical feed value