Band 3 protein disorder in one family with G6PD enzyme deficiency

Purpose: Proteins found in complex membrane structure determines its stability and flexibility. The structural defect and deficiencies observed on erythrocyte membrane cause hemolytic anemias. In this study the erythrocyte membrane proteins of a family having G6PD enzyme deficiency were studied to investigate its possible contribution to hemolysis. Methods: The enzyme activity was measured by using Beutler method and red cell membrane proteins were investigated by SDS-PAGE in 8.3% gels according to Fairbanks et al. Results: G6PD enzyme deficiency was found in all members of family, except for the father. While as an erythrocyte membrane protein band 3 protein deficiency was observed in the mother, father and two children, but one child was found to be normal. Also the mother and 5 years old son were sickle cell carrier, and the father has ?-thalassemia-2 (?3.7) Conclusion: The results showed that there may be associations between erythrocyte membrane proteins and hemolytic anemias such as G6PD deficiency and hemoglobinopathies

3 NEW G6PD VARIANTS, G6PD ADANA, G6PD SAMANDAG, AND G6PD BALCALI IN CUKUROVA, TURKEY

WOS: A1987H778600019PubMed ID: 3610149

STUDIES ON RED-CELL GLUCOSE-6-PHOSPHATE-DEHYDROGENASE - EVALUATION OF REFERENCE VALUES

WOS: A1994MU13700009PubMed ID: 8154852The analytical, intra-individual, inter-individual variation and reference values were determined for red cell glucose-6-phosphate dehydrogenase (G6PD). Different procedures for the conditions for storage of red blood cells and the preparation of haemolysates were investigated. A total of 2170 samples of blood were taken from apparently healthy persons-1212 males and 958 females-from randomly selected villages and city centres in the southern part of Turkey. Analytical variation, intra-individual variation and inter-individual variation were 8.67%, 32.8% and 31.8%, respectively. The mean (SD) for G6PD was 8.6 (3.3) IU/gHb. The index of individuality, 1.03, showed that the reference intervals could be used for diagnostic purposes. Whole blood or a red cell pellet could be stored in physiological saline for one week at 4 degrees C or -20 degrees with little loss of activity. Two of three different procedures for the preparation of haemolysate gave data that showed no statistical difference and were equally satisfactory

Correlation between trace elements and lipid profiles

The aim of this study was to examine the plasma Cu, Zn, and Mg levels in three different groups (control, hyperlipidemic, and hypercholesterolemic) and to determine any correlation between these parameters and the level of triglyceride (T.G), cholesterol (Chol), LDL, and HDL. For this purpose, serum Cu, Zn, and Mg levels were determined by atomic absorbtion spectrophotometry in three groups: Group I (n = 35 control, normal lipid profile); Group II (n = 36 hyperlipidemic); Group III (n = 15 familial hypercholesterolemia), and the results were statistically analyzed. The results and their statistical evaluation for the lipid and plasma Cu, Zn, and Mg levels were as follows: Only statistically significant differences were observed between Group I and Group II for T.G, Chol, LDL, and HDL, but no statistically significant differences were observed between the other groups and parameters. When associations between T.G, Chol, LDL, HDL, and plasma Cu, Zn, Mg were examined, significant correlations were observed between Cu and T.G (r = 0.356), Cu and Chol (r = 0.828), Cu and LDL (r = 0.806) in Group III; Zn and HDL (r = 0.543) in Group I, Zn and Chol (r = 0.378), Zn and LDL (r = 0.538) in Group III; Mg and T.G (r = 0.354), Mg and Chol (r = 0.444), Mg and LDL (r = 0.433), Mg and HDL (r = 0.375) in Group I

CORRELATION BETWEEN TRACE-ELEMENTS AND LIPID PROFILES

WOS: A1994QK13300003The aim of this study was to examine the plasma Cu, Zn, and Mg levels in three different groups (control, hyperlipidemic, and hypercholesterolemic) and to determine any correlation between these parameters and the level of triglyceride (T.G), cholesterol (Chol), LDL, and HDL. For this purpose, serum Cu, Zn, and Mg levels were determined by atomic absorbtion spectrophotometry in three groups: Group I (n = 35 control, normal lipid profile); Group II (n = 36 hyperlipidemic); Group III (n = 15 familial hypercholesterolemia), and the results were statistically analyzed. The results and their statistical evaluation for the lipid and plasma Cu, Zn, and Mg levels were as follows: Only statistically significant differences were observed between Group I and Group II for T.G, Chol, LDL, and HDL, but no statistically significant differences were observed between the other groups and parameters. When associations between T.G, Chol, LDL, HDL, and plasma Cu, Zn, Mg were examined, significant correlations were observed between Cu and T.G (r = 0.356), Cu and Chol (r = 0.828), Cu and LDL (r = 0.806) in Group III; Zn and HDL (r = 0.543) in Group I, Zn and Chol (r = 0.378), Zn and LDL (r = 0.538) in Group III; Mg and T.G (r = 0.354), Mg and Chol (r = 0.444), Mg and LDL (r = 0.433), Mg and HDL (r = 0.375) in Group I. (C) 1995 Wiiey-Liss, Inc

Oxidative Stress and Apoptosis in Relation to Exposure to Magnetic Field

PubMedID: 20824388We investigated the effect of extremely low-frequency electromagnetic field (ELF-EMF) with pulse trains exposure on lipid peroxidation, and, hence, oxidative stress in the rat liver tissue. The parameters that we measured were the levels of plasma alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase as well as plasma albumin, bilirubin, and total protein levels in 30 adult male Wistar rats exposed to ELF. We also determined the percentage of apoptotic and necrotic cells of the kidney extracts from the animals by flow cytometry method. Apoptotic cell death was further characterized by monitoring DNA degradation using gel electrophoresis. The results showed an increase in the levels of oxidative stress indicators, and the flow cytometric data suggested a possible relationship between the exposure to magnetic field and the cell death. We showed significantly lower necrotic cell percentages in experimental animals compared to either unexposed or sham control groups. However, DNA ladder analyses did not differentiate between the groups. Our results were discussed in relation to the response of biological systems to EMF. © 2010 Springer Science+Business Media, LLC.Acknowledgment The authors acknowledge with thanks the support provided by the Biochemistry department of Cukurova Medical School by way of valuable contributions in the determination of oxidative stress parameters