Dual-topology insertion of a dual-topology membrane protein.

Some membrane transporters are dual-topology dimers in which the subunits have inverted transmembrane topology. How a cell manages to generate equal populations of two opposite topologies from the same polypeptide chain remains unclear. For the dual-topology transporter EmrE, the evidence to date remains consistent with two extreme models. A post-translational model posits that topology remains malleable after synthesis and becomes fixed once the dimer forms. A second, co-translational model, posits that the protein inserts in both topologies in equal proportions. Here we show that while there is at least some limited topological malleability, the co-translational model likely dominates under normal circumstances

Magnetowave Induced Plasma Wakefield Acceleration for Ultra High Energy Cosmic Rays

Magnetowave induced plasma wakefield acceleration (MPWA) in a relativistic astrophysical outflow has been proposed as a viable mechanism for the acceleration of cosmic particles to ultra high energies. Here we present simulation results that clearly demonstrate the viability of this mechanism for the first time. We invoke the high frequency and high speed whistler mode for the driving pulse. The plasma wakefield so induced validates precisely the theoretical prediction. We show that under appropriate conditions, the plasma wakefield maintains very high coherence and can sustain high-gradient acceleration over a macroscopic distance. Invoking gamma ray burst (GRB) as the source, we show that MPWA production of ultra high energy cosmic rays (UHECR) beyond ZeV 10^21 eV is possible.Comment: 4 pages, 4 figure

Spin Squeezing under Non-Markovian Channels by Hierarchy Equation Method

We study spin squeezing under non-Markovian channels, and consider an ensemble of $N$ independent spin-1/2 particles with exchange symmetry. Each spin interacts with its own bath, and the baths are independent and identical. For this kind of open system, the spin squeezing under decoherence can be investigated from the dynamics of the local expectations, and the multi-qubit dynamics can be reduced into the two-qubit one. The reduced dynamics is obtained by the hierarchy equation method, which is a exact without rotating-wave and Born-Markov approximation. The numerical results show that the spin squeezing displays multiple sudden vanishing and revival with lower bath temperature, and it can also vanish asymptotically.Comment: 7 pages, 4 figure

“An ethnographic seduction”: how qualitative research and Agent-based models can benefit each other

We provide a general analytical framework for empirically informed agent-based simulations. This methodology provides present-day agent-based models with a sound and proper insight as to the behavior of social agents — an insight that statistical data often fall short of providing at least at a micro level and for hidden and sensitive populations. In the other direction, simulations can provide qualitative researchers in sociology, anthropology and other fields with valuable tools for: (a) testing the consistency and pushing the boundaries, of specific theoretical frameworks; (b) replicating and generalizing results; (c) providing a platform for cross-disciplinary validation of results

Gardnerella vaginalis promotes group B Streptococcus vaginal colonization, enabling ascending uteroplacental infection in pregnant mice

BACKGROUND: Group B Streptococcus is a common vaginal bacterium and the leading cause of invasive fetoplacental infections. Group B Streptococcus in the vagina can invade through the cervix to cause ascending uteroplacental infections or can be transmitted to the neonate during vaginal delivery. Some studies have found that women with a dysbiotic polymicrobial or Lactobacillus-depleted vaginal microbiota are more likely to harbor group B Streptococcus. Gardnerella vaginalis is often the most abundant bacteria in the vaginas of women with dysbiosis, while being detected at lower levels in most other women, and has been linked with several adverse pregnancy outcomes. Mouse models of group B Streptococcus and Gardnerella vaginalis colonization have been reported but, to the best of our knowledge, the two have not been studied together. The overarching idea driving this study is that certain members of the dysbiotic vaginal microbiota, such as Gardnerella vaginalis, may directly contribute to the increased rate of group B Streptococcus vaginal colonization observed in women with vaginal dysbiosis. OBJECTIVE: We used a mouse model to test the hypothesis that vaginal exposure to Gardnerella vaginalis may facilitate colonization and/or invasive infection of the upper reproductive tract by group B Streptococcus during pregnancy. STUDY DESIGN: Timed-pregnant mice were generated using an allogeneic mating strategy with BALB/c males and C57Bl/6 females. Dams were vaginally inoculated at gestational day 14 with group B Streptococcus alone (using a 10-fold lower dose than previously reported models) or coinoculated with group B Streptococcus and Gardnerella vaginalis. Bacterial titers were enumerated in vaginal, uterine horn, and placental tissues at gestational day 17. The presence (Fisher exact tests) and levels (Mann-Whitney U tests) of bacterial titers were compared between mono- and coinoculated dams in each compartment. Relative risks were calculated for outcomes that occurred in both groups. Tissue samples were also examined for evidence of pathophysiology. RESULTS: Inoculation of pregnant mice with 10 CONCLUSION: These data suggest that Gardnerella vaginalis vaginal exposure can promote group B Streptococcus vaginal colonization, resulting in a greater likelihood of invasive perinatal group B Streptococcus infections. These findings suggest that future clinical studies should examine whether the presence of Gardnerella vaginalis is a risk factor for group B Streptococcus vaginal colonization in women. Because Gardnerella vaginalis can also be present in women without bacterial vaginosis, these findings may be relevant both inside and outside of the context of vaginal dysbiosis

Should the Nordic Countries Join A European Monetary Union? An Empirical Analysis.

This paper examines the implications for the Nordic Countries (Denmark, Finland, Norway and Sweden) of participating in the finan stage of the European Monetary Union. Economic linkages with Germany are estimated using a time series approach under both the Bretton Woods and the post-Bretton Woods exchange rate regimes. Output responses of the Nordic countries to permanent and transitory disturbances are estimated and compared with two small "core" members, Belgium and the Netherlands. We find that the long-standing EU members (Belgium, Denmark and the Netherlands) are closely integrated with Germany in that German shocks have a direct and large impact on their output developments. These linkages appear much weaker for Finland, Norway and Sweden. Common European disturbances also do not distinguish the Nordic countries from the non-Nordic countries.

Propagation Characteristics of International Space Station Wireless Local Area Network

This paper describes the application of the Uniform Geometrical Theory of Diffraction (UTD) for Space Station Wireless Local Area Networks (WLANs) indoor propagation characteristics analysis. The verification results indicate good correlation between UTD computed and measured signal strength. It is observed that the propagation characteristics are quite different in the Space Station modules as compared with those in the typical indoor WLANs environment, such as an office building. The existing indoor propagation models are not readily applicable to the Space Station module environment. The Space Station modules can be regarded as oversized imperfect waveguides. Two distinct propagation regions separated by a breakpoint exist. The propagation exhibits the guided wave characteristics. The propagation loss in the Space Station, thus, is much smaller than that in the typical office building. The path loss model developed in this paper is applicable for Space Station WLAN RF coverage and link performance analysis

A progressive diagonalization scheme for the Rabi Hamiltonian

A diagonalization scheme for the Rabi Hamiltonian, which describes a qubit interacting with a single-mode radiation field via a dipole interaction, is proposed. It is shown that the Rabi Hamiltonian can be solved almost exactly using a progressive scheme that involves a finite set of one variable polynomial equations. The scheme is especially efficient for lower part of the spectrum. Some low-lying energy levels of the model with several sets of parameters are calculated and compared to those provided by the recently proposed generalized rotating-wave approximation and full matrix diagonalization.Comment: 8pages, 1 figure, LaTeX. Accepted for publication in J. Phys. B: At. Mol. Opt. Phy