A novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system

There is an increasing demand for easy, high-throughput (HTP) methods for protein engineering to support advances in the development of structural biology, bioinformatics and drug design. Here, we describe an N- and C-terminal cloning method utilizing Gateway cloning technology that we have adopted for chimeric and mutant genes production as well as domain shuffling. This method involves only three steps: PCR, in vitro recombination and transformation. All three processes consist of simple handling, mixing and incubation steps. We have characterized this novel HTP method on 96 targets with >90% success. Here, we also discuss an N- and C-terminal cloning method for domain shuffling and a combination of mutation and chimeragenesis with two types of plasmid vectors

コウダイ レンケイ ニ ヨル セツゾクキョウイク プログラム カイハツ ノ ココロミ

高大連携により効率的な接続教育プログラムを開発するため,高校向けアンケートを実施した。職業に就いた際に必要とされるキャリア基礎能力として本学で過去実施したアンケート結果を基にした10 要素,ならびに経済産業省の「社会人基礎力」12 要素の必要性と達成度を設問とした。その結果,社会人にもっとも必要とされるキャリア基礎能力は「コミュニケーション能力」であること,また,高校教科「情報」によりPC 基礎操作能力は十分獲得されていること,がわかった。この結果を踏まえ,高大連携プログラムの一環として高校-大学教員によるコミュニケーション教育研究会を開催し,全12 講からなる接続教育プログラム「コミュニケーションリテラシー」を開講した。Enquiries were made to upper secondary school teachers on basic vocational abilities necessary for thegraduates. Since "information literacy" was introduced recently as compulsory subject in secondary schools, itsrelation with elements necessary for an ordinary person who wishes to work in a company was investigated. Basedon the results, a project was set up with members from secondary school teachers and Shohoku College teachersand library staff. As a result, a novel coordination program, namely, "communication literacy", consisting of 12lessons, was held for secondary school third grade students

コウダイレンケイ ニ ヨル キョウイク コウリュウ ネットワーク ノ コウチク : コミュニケーション キョウイク ケンキュウカイ ノ カツドウ ト コミュニケーションリテラシー

高大連携校より本学への入学が決定した生徒を対象とした単位認定科目「コミュニケーションリテラシー」を開講した。同科目の内容やスケジュールは、平成19 年度よりほぼ月に一度のペースで高大連携協定校教員と本学教職員で構成する「コミュニケーション教育研究会」で調査研究・見学・参観・討議などを重ねて決定されたものである。この研究会の中ではまた、ブレインストーミングにより、経済産業省の「社会人基礎能力」を基本とした「職業を持った社会人に必要とされる能力要素」の洗い出しを行ない、これら要素の必要性や獲得度のアンケート調査を実施した。対象は高大連携校と全国の高校である。その結果、職業をもった社会人にもっとも必要とされる基本的な基礎能力は、「コミュニケーション能力」と「一般常識」であること、さらには「課題発見能力や企画・提案能力」や「表現力やプレゼンテーション能力」が不十分であるととらえられていることがわかった。こうした問題意識を踏まえて「コミュニケーションリテラシー」の実施内容を組み立てた。本年度の「コミュニケーションリテラシー」受講生は110 名となった。この効果を検証することは容易ではないが、受講生に対しアンケート調査などを行った結果に関しても報告する。Novel coordination program, namely, "Communication Literacy", consisting of 15 lesson classes, was developed and held for secondary school third grade students. This class was developed and implemented in a project set up with members from secondary school teachers and Shohoku College teachers and staff. Survey on basic vocational abilities necessary for the graduates was made by sending questionnaires to secondary school teachers. Questionnaires were made to students participated in the program to clarify the effect of the program

Effectiveness of time-varying echo information for target geometry identification in bat-inspired human echolocation.

Bats use echolocation through flexible active sensing via ultrasounds to identify environments suitable for their habitat and foraging. Mimicking the sensing strategies of bats for echolocation, this study examined how humans acquire new acoustic-sensing abilities, and proposes effective strategies for humans. A target geometry identification experiment-involving 15 sighted people without experience of echolocation-was conducted using two targets with different geometries, based on a new sensing system. Broadband frequency-modulated pulses with short inter-pulse intervals (16 ms) were used as a synthetic echolocation signal. Such pulses mimic buzz signals emitted by bats for echolocation prior to capturing their prey. The study participants emitted the signal from a loudspeaker by tapping on Android devices. Because the signal included high-frequency signals up to 41 kHz, the emitted signal and echoes from a stationary or rotating target were recorded using a 1/7-scaled miniature dummy head. Binaural sounds, whose pitch was down-converted, were presented through headphones. This way, time-varying echo information was made available as an acoustic cue for target geometry identification under a rotating condition, as opposed to a stationary one. In both trials, with (i.e., training trials) and without (i.e., test trials) answer feedback immediately after the participants answered, the participants identified the geometries under the rotating condition. Majority of the participants reported using time-varying patterns in terms of echo intensity, timbre, and/or pitch under the rotating condition. The results suggest that using time-varying patterns in echo intensity, timbre, and/or pitch enables humans to identify target geometries. However, performance significantly differed by condition (i.e., stationary vs. rotating) only in the test trials. This difference suggests that time-varying echo information is effective for identifying target geometry through human echolocation especially when echolocators are unable to obtain answer feedback during sensing