Host-Tailored Sensors for Leucomalachite Green Potentiometric Measurements

A new biomimetic sensor for leucomalachite green host-guest interactions and potentiometric transduction is presented. The artificial host was imprinted in methacrylic acid or acrylamido-2-methyl-1-propanesulfonic acid-based polymers. Molecularly imprinted particles were dispersed in 2-nitrophenyloctyl ether and trapped in poly(vinyl chloride). The potentiometric sensors exhibited a near-Nernstian response in steady state evaluations, with slopes and detection limits ranging from 45.8 to 81.2 mV and 0.28 to 1.01 , respectively. They were independent from the pH of test solutions within 3 to 5. Good selectivity was observed towards drugs that may contaminate water near fish cultures, such as oxycycline, doxycycline, enrofloxacin, trimethoprim, creatinine, chloramphenicol, and dopamine. The sensors were successfully applied to field monitoring of leucomalachite green in river samples. The method offered the advantages of simplicity, accuracy, applicability to colored and turbid samples, and automation feasibility

Application of a Monte Carlo lung dosimetry code to the inhalation of thoron progeny.

To determine radiation doses incurred by inhaled thoron progeny, the Monte Carlo radon progeny lung dosimetry code IDEAL-DOSE was adapted to the inhalation of thoron progenies, comprising the alpha-emitting nuclides (216)Po, (212)Bi and (212)Po. Dose calculations for defined exposure conditions yielded a dose conversion coefficient (DCC) of 4.6 mSv WLM(-1) or 94.2 nSv (Bq h m(-3))(-1) when compared with a DCC of 3.8 mSv WLM(-1) if based on the International Commission on Radiological Protection Human Respiratory Tract Model. Bronchial doses were computed for different thoron progenies exposure conditions measured in a Bavarian half-timbered house and in a thoron experimental house at the Helmholtz Zentrum München. DCCs ranged from 4.9 to 12.9 mSv WLM(-1), depending on particle size, unattached fraction and fractional activity concentrations. For exposure-specific indoor aerosol parameters, the thoron progeny DCC is smaller than the radon progeny DCC by about a factor of 2

Coniferous Trees as Bioinspiration for Designing Long Reinforced Prestressed Concrete Columns

This article contains the results of identifying the potential of coniferous trees to act as bioinspiration for the structural design of columns in single-story warehouses subjected to high wind velocity and severe seismic action. This study starts by analyzing the biomechanics of coniferous trees, continues with an abstraction of the relevant features, and ends with the transfer of a design methodology for long reinforced and prestressed concrete columns. To verify the applicability and validity of the mathematical relationships extracted from the bibliographic study to characterize the biomechanics of coniferous trees, a study site is conducted for Norway spruce trees felled by the wind in the Bilbor area. The design methodology for long reinforced and prestressed concrete columns bioinspired by the Norway spruce trees is experimentally validated using two case studies. The first case study deals with the effect of centric prestressing on long concrete columns, and the second on the influence of the walnut shell powder on the adhesion of the reinforcement in concrete. The case studies presented aim to transfer some characteristics from trees to reinforced concrete to improve the performance of long columns under horizontal forces. The results obtained indicate a good approximation of the trees’ structural behavior for this site and for ones investigated by other researchers in different forests

Clinical and pathological implications of miRNA in bladder cancer

Cornelia Braicu,1 Roxana Cojocneanu-Petric,1,2 Sergiu Chira,1 Anamaria Truta,1,3 Alexandru Floares,4 Bogdan Petrut,5,6 Patriciu Achimas-Cadariu,7,8,* Ioana Berindan-Neagoe1,9–11,*1Research Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania; 2Faculty of Biology and Geology, Babes-Bolyai University, Cluj-Napoca, Romania; 3Department of Medical Genetics, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania; 4Solutions of Artificial Intelligence Applications, Cluj-Napoca, Romania; 5Department of Urology, The Oncology Institute “ Prof Dr. Ion Chiricuta”, Cluj-Napoca, Romania; 6Department of Urology, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania; 7Department of Surgery, The Oncology Institute “ Prof Dr. Ion Chiricuta”, Cluj-Napoca, Romania; 8Department of Surgical Oncology and Gynaecological Oncology, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania; 9Department of Immunology, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania; 10Department of Functional Genomics and Experimental Pathology, The Oncology Institute “ Prof Dr. Ion Chiricuta”, Cluj-Napoca, Romania; 11Department of Experimental Therapeutics M.D. Anderson Cancer Center Houston, TX, USAAbstract: MicroRNAs (miRNAs) are small, noncoding RNA species with a length of 20–22 nucleotides that are recognized as essential regulators of relevant molecular mechanisms, including carcinogenesis. Current investigations show that miRNAs are detectable not only in different tissue types but also in a wide range of biological fluids, either free or trapped in circulating microvesicles. miRNAs were proven to be involved in cell communication, both in pathological and physiological processes. Evaluation of the global expression patterns of miRNAs provides key opportunities with important practical applications, taking into account that they modulate essential biological processes such as epithelial to mesenchymal transition, which is a mechanism relevant in bladder cancer. miRNAs collected from biological specimens can furnish valuable evidence with regard to bladder cancer oncogenesis, as they also have been linked to clinical outcomes in urothelial carcinoma. Therefore, a single miRNA or a signature of multiple miRNAs may improve risk stratification of patients and may supplement the histological diagnosis of urological tumors, particularly for bladder cancer.Keywords: bladder cancer, miRNA, prognostic, diagnosti