ENZYME SENSORS FOR BIOTECHNOLOGICAL PROCESSES AND PRODUCTS

Enzyme sensors have been developed usina coupled enzyme reactions to determine the concentrations of the biotechnoloay products starch, pullulan, oligosaccharides. maltose. the low-calorie sweetener maltitol. glutamine, and enzyme activities of alpha-amylase and pullulanase. These enzyme sensors have been tested to control enzyme production by immobilized cells and also animal tissue cultures

ENZYME- AND IMMUNO-FIA-CONTROL OF ANIMAL CELL CULTURES

Three enzyme sensors (for glucose, lactate and glutamine) have been coupled simultaneously with a flow-injection analysis device on-line with bioreactors harboring monoclonal antibody and interleukin-2 producing cell lines. Two approaches for FIA-coupled immunosensors were demonstrated for detection of alpha-interferon