Elephants and pandemics

Baker & Winkler’s critique of Asian elephant tourism and conservation in Thailand has convinced me that this was “an industry with too many victims.” Yet I fear that B&W’s proposed remedy of returning to past elephant husbandry by Karen hill-peoples has little likelihood of improving the lives of the elephants for long. Who can predict whether the Karen will live up to this hope? B&W advocate for the Karen, but not for “an abolitionist stance on elephant-human relationships.” In my view, whether we discuss elephants or the wild mammals that carry SARS-CoV-2, abolition of many human uses of animals is needed to save our planet and ourselves. Are Karen elephant-keepers that different from the bat-hunters who seem to have brought the pandemic to our doorsteps? Both eke out a marginal living in traditional ways exploiting animals. Human survival does not depend on sustaining “wet markets” or elephant tourism or eating meat every day. What we really need is fundamental reform of our relationships with animals, starting with the human governance of the nonhuman world that sustains us all. Elephants and pandemics expose the myth of limitless human freedom

Elephants and pandemics

Baker & Winkler’s critique of Asian elephant tourism and conservation in Thailand has convinced me that this was “an industry with too many victims.” Yet I fear that B&W’s proposed remedy of returning to past elephant husbandry by Karen hill-peoples has little likelihood of improving the lives of the elephants for long. Who can predict whether the Karen will live up to this hope? B&W advocate for the Karen, but not for “an abolitionist stance on elephant-human relationships.” In my view, whether we discuss elephants or the wild mammals that carry SARS-CoV-2, abolition of many human uses of animals is needed to save our planet and ourselves. Are Karen elephant-keepers that different from the bat-hunters who seem to have brought the pandemic to our doorsteps? Both eke out a marginal living in traditional ways exploiting animals. Human survival does not depend on sustaining “wet markets” or elephant tourism or eating meat every day. What we really need is fundamental reform of our relationships with animals, starting with the human governance of the nonhuman world that sustains us all. Elephants and pandemics expose the myth of limitless human freedom

The metabolomic profile of gamma-irradiated human hepatoma and muscle cells reveals metabolic changes consistent with the Warburg effect

The two human cell lines HepG2 from hepatoma and HMCL-7304 from striated muscle were γ-irradiated with doses between 0 and 4 Gy. Abundant γH2AX foci were observed at 4 Gy after 4 h of culture post-irradiation. Sham-irradiated cells showed no γH2AX foci and therefore no signs of radiation-induced double-strand DNA breaks. Flow cytometry indicated that 41.5% of HepG2 cells were in G2/M and this rose statistically significantly with increasing radiation dose reaching a plateau at ∼47%. Cell lysates from both cell lines were subjected to metabolomic analysis using Gas Chromatography-Mass Spectrometry (GCMS). A total of 46 metabolites could be identified by GCMS in HepG2 cell lysates and 29 in HMCL-7304 lysates, most of which occurred in HepG2 cells. Principal Components Analysis (PCA) showed a clear separation of sham, 1, 2 and 4 Gy doses. Orthogonal Projection to Latent Structures-Discriminant Analysis (OPLS-DA) revealed elevations in intracellular lactate, alanine, glucose, glucose 6-phosphate, fructose and 5-oxoproline, which were found by univariate statistics to be highly statistically significantly elevated at both 2 and 4 Gy compared with sham irradiated cells. These findings suggested upregulation of cytosolic aerobic glycolysis (the Warburg effect), with potential shunting of glucose through aldose reductase in the polyol pathway, and consumption of reduced Glutathione (GSH) due to γ-irradiation. In HMCL-7304 myotubes, a putative Warburg effect was also observed only at 2 Gy, albeit a lesser magnitude than in HepG2 cells. It is anticipated that these novel metabolic perturbations following γ-irradiation of cultured cells will lead to a fuller understanding of the mechanisms of tissue damage following ionizing radiation exposure

Just preservation, trusteeship and multispecies justice

We are grateful to all the commentators who engaged with our target article. Some commentators have offered important insights into our proposed design and methods for legally intervening on behalf of futurity. Others have focused on theoretical considerations central to our proposal for multispecies justice and trusteeship. All have inspired modifications and further elaboration of our initial proposal. In this Response, we engage with the commentaries, integrating their suggestions, striving for convergence and complementarity, but also discussing points of divergence with our proposed framework where necessary. There is substantial overlap in the points of view of the three co-authors, but there are also differences. Section 1 is more reflective of the views of AT and Section 2 is more reflective of the views of FJS-A and WL

Just preservation

We are failing to protect the biosphere. Novel views of conservation, preservation, and sustainability are surfacing in the wake of consensus about our failures to prevent extinction or slow climate change. We argue that the interests and well-being of non-humans, youth, and future generations of both human and non-human beings (futurity) have too long been ignored in consensus-based, anthropocentric conservation. Consensus-based stakeholder-driven processes disadvantage those absent or without a voice and allow current adult humans and narrow, exploitative interests to dominate decisions about the use of nature over its preservation for futurity of all life. We propose that authentically non-anthropocentric worldviews that incorporate multispecies justice are needed for a legitimate, deliberative, and truly democratic process of adjudication between competing interests in balancing the preservation and use of nature. Legitimate arenas for such adjudication would be courts that can defend intergenerational equity, which is envisioned by many nations\u27 constitutions, and can consider current and future generations of non-human life. We urge practitioners and scholars to disavow implicit anthropocentric value judgments in their work – or make these transparent and explicit – and embrace a more comprehensive worldview that grants future life on earth fair representation in humanity\u27s decisions and actions today

Mismeasured Mortality: Correcting Estimates of Wolf Poaching in the United States

Measuring rates and causes of mortalities is important in animal ecology and management. Observing the fates of known individuals is a common method of estimating life history variables, including mortality patterns. It has long been assumed that data lost when known animals disappear were unbiased. We test and reject this assumption under conditions common to most, if not all, studies using marked animals. We illustrate the bias for 4 endangered wolf populations in the United States by reanalyzing data and assumptions about the known and unknown fates of marked wolves to calculate the degree to which risks of different causes of death were mismeasured. We find that, when using traditional methods, the relative risk of mortality from legal killing measured as a proportion of all known fates was overestimated by 0.05–0.16 and the relative risk of poaching was underestimated by 0.17–0.44. We show that published government estimates are affected by these biases and, importantly, are underestimating the risk of poaching. The underestimates have obscured the magnitude of poaching as the major threat to endangered wolf populations. We offer methods to correct estimates of mortality risk for marked animals of any taxon and describe the conditions under which traditional methods produce more or less bias. We also show how correcting past and future estimates of mortality parameters can address uncertainty about wildlife populations and increase the predictability and sustainability of wildlife management interventions

Leopards and mesopredators as indicators of mammalian species richness across diverse landscapes of South Africa

The rapid extinction of species over the past few decades has created a biodiversity crisis. Factors contributing to recent extirpations are linked to increased human population growth, habitat loss and fragmentation, and over-exploitation of wildlife. Only decisive, effective action to combat biodiversity loss can reverse these trends. The use of indicator species as surrogates for biodiversity provides a way to identify areas with high biodiversity so that conservation efforts can be accelerated and supported in those areas. Predators are considered important indicators of healthy, biodiverse ecosystems due to their high trophic level and their direct and indirect interaction with other species. Using camera trap data from 221 cameras set across five vegetation types and five land use zones in South Africa, we evaluated carnivores as potential surrogates for biodiversity

Quantifying the effects of delisting wolves after the first state began lethal management

Predators and their protection are controversial worldwide. Gray wolves, Canis lupus, lost U.S. federal protection (delisting) and the State of Wisconsin began lethal management first among all states and tribes that regained authority over wolves. Here we evaluated the initial success of reaching the state’s explicit objective, “…to allow for a sustainable harvest that neither increases nor decreases the state’s wolf population…” We used official state figures for hunter-killed wolves, population estimates from April 2017–2020, and the latest peer-reviewed model of individual wolf survival to estimate additional deaths resulting from federal delisting. More than half of the additional deaths were predicted to be cryptic poaching under the assumption that this period resembled past periods of liberalized wolf-killing in Wisconsin. We used a precautionary approach to construct three conservative scenarios to predict the current status of this wolf population and a minimum estimate of population decline since April 2020. From our scenarios that vary in growth rates and additional mortality estimates, we expect a maximum of 695–751 wolves to be alive in Wisconsin by 15 April 2021, a minimum 27–33% decline in the preceding 12 months. This contradicts the state expectation of no change in the population size. We draw a conclusion about the adequacy of regulatory mechanisms under state control of wolves and discuss the particular governance conditions met in Wisconsin. We recommend greater rigor and independent review of the science used by agencies to plan wolf hunting quotas and methods. We recommend clearer division of duties between state wildlife agencies, legislatures, and courts. We recommend federal governments reconsider the practice of sudden deregulation of wolf management and instead recommend they consider protecting predators as non-game or transition more slowly to subnational authority, to avoid the need for emergency relisting

Factors predicting habitat use by leopards in human-altered landscapes

SUPPLEMENTARY DATA SD1.—Univariate analyses results for male and female leopard occurrence probability at the fine and large spatial scales.Transformed landscapes caused by human activity leave remnant patches of natural habitat for wildlife. The persistence of species in the face of such transformation depends on individuals’ ability to adapt to novel habitat, and to secure resources and reproductive opportunities despite habitat alterations. The leopard, Panthera pardus, is the last free-roaming top carnivore in South Africa whose high trophic status and wide-ranging movements make them an effective focal species in conservation planning. Using location data from leopards, we investigated key correlates of habitat selection in human-altered landscapes at two spatial scales. We compared sex-related differences and predicted how conspecific home range locations influenced habitat selection. Leopards avoided human-altered landscapes more strongly at the large spatial scale, where both sexes selected core areas near formally protected areas. Conspecific home range locations had a strong positive effect at both spatial scales for males, while for females, conspecifics explained fine-scale habitat selection by selecting areas near neighboring females. Spatial scale, sex-related differences, and conspecific location play roles in habitat selection for solitary felids and have implications for conservation planning and management. Excluding these factors may result in inappropriate species management policies.Development Bank South Africa, United Nations Environmental Programme, United Nations Development Programme, Green Fund, Global Environmental Facility, Global Environment Facility/Small Grants Programme, Henry and Iris Englund Foundation, National Lotteries Distribution Trust Fund, Mones Michaels Trust, Tamarisk Trust, Timothy Allsop, ABAX Foundation, Table Mountain Fund, Vodacom Foundation, Deutsche Bank Foundation (RSA), Brad Banducci, and Arne Hanson.https://academic.oup.com/jmammalhj2023Mammal Research Institut