Physiological and Agronomical Responses of ‘Vairo’ Almond and ‘Big Top’ Nectarine Cultivars Grafted onto Different Prunus Rootstocks and Grown under Semiarid Mediterranean Conditions

Two trials were conducted under Mediterranean conditions to monitor several physiological indicators before harvest (leaf chlorophyll concentration, quantum yield of photosystem II electron transport, stem water potential, and stomatal conductance) and some agronomic performance parameters before and at harvest (vigor, fruit growth, fruit size, fruit weight, and yield), of ‘Vairo’ almond and ‘Big Top’ nectarine cultivars grafted onto eight Prunus rootstocks, six of which are common in both cultivars. For both ‘Vairo’ almond and ‘Big Top’ nectarine cultivars, factors including rootstock, date, and the interaction between rootstock and date, from fruit set to harvest were evaluated. Significantly affected were certain physiological and agronomical traits which were evaluated before harvest, with stem water potential being the parameter affected by interaction in both cultivars. In fact, the stem water potential presented low levels in Rootpac-20 and high levels in Rootpac-40 for both cultivars. With regard to the other physiological traits evaluated during the growing period, changes in stomatal conductance were observed in ‘Vairo’, but not in ‘Big Top’. Comparing rootstocks throughout the season, Rootpac-40 and IRTA-1 exhibited the highest stomatal conductance values, whereas the lowest was observed in Rootpac-R; Rootpac-20 and Ishtara also presented low values. Regarding agronomical traits at harvest, GF-677 and IRTA-1 produced high yields for ‘Vairo’ almond cultivar, whereas Rootpac-40 and Ishtara performed better with ‘Big Top’ nectarine cultivar.info:eu-repo/semantics/publishedVersio

Survey of Oomycetes Associated with Root and Crown Rot of Almond in Spain and Pathogenicity of Phytophthora niederhauserii and Phytopythium vexans to `Garnem¿ Rootstock

[EN] From 2018 to 2020, surveys of oomycetes associated with root and crown rot of almond (Prunus dulcis) were conducted on diseased young almond trees in commercial orchards and nurseries in six provinces of Spain. A total of 104 oomycete isolates were obtained from plant and soil samples, which h were identified by sequencing the internal transcribed spacer (ITS) region of the ribosomal DNA. Diverse species belonging to the genera Globisporangium, Phytophthora, Phytopythium and Pythium were found, Phytopythium vexans and Phytophthora niederhauserii being the most frequent. The pathogenicity of these two species to one-year-old almond seedlings of 'Garnem' (P. dulcis x P. persica) rootstock was studied. All seedlings inoculated with Pp. vexans and Ph. niederhauserii isolates showed severe symptoms at the late stage of the pathogenicity test (defoliation, wilting and dieback) and several plants died. Some isolates of Ph. niederhauserii significantly reduced the dry weight of the roots compared with the control, but this effect was not observed in seedlings inoculated with Pp. vexans. These results provide new information about the oomycete species present in almond crops in Spain and highlight the importance of carrying out frequent phytosanitary surveys for a better knowledge of potential risks posed by these soil-borne pathogens.This research study was funded by INIA (Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria), Spain, through projects RTA2017-00009-C04-01 and RTA2017-00009-C04-04, and matching funds from the ERDF (European Regional Development Fund) and Grant PID2020-114648RR-C33 funded by MCIN/AEI/10.13039/501100011033. Francisco Beluzán was supported by Agencia Nacional de Investigación y Desarrollo/Subdirección de Capital Humano/Doctorado Becas Chile en el Extranjero/72200145. Xavier Miarnau was supported by the CERCA Program, Generalitat de Catalunya.Beluzán Flores, FJ.; Miarnau, X.; Torguet, L.; Armengol Fortí, J.; Abad Campos, P. (2022). Survey of Oomycetes Associated with Root and Crown Rot of Almond in Spain and Pathogenicity of Phytophthora niederhauserii and Phytopythium vexans to `Garnem¿ Rootstock. Agriculture. 12(2):1-15. https://doi.org/10.3390/agriculture12020294S11512

Evaluation of Fungicides and Application Strategies for the Management of the Red Leaf Blotch Disease of Almond

Red leaf blotch (RLB) of almond, caused by Polystigma amygdalinum, is an important foliar disease of this nut tree in the Mediterranean basin and especially in Spain. In recent years, the control of this disease has become a key factor in the management of Spanish almond orchards. The management of RLB is not easy due to intrinsic factors of the disease (e.g., long infection and latency periods) and the low number of registered fungicides in this country. From 2015 to 2019, different field trials were conducted in the Lleida region, NE Spain, to evaluate the efficacy of several fungicide products and of application strategies to control this disease. Systemic fungicides, which included fluopyram, trifloxystrobin, and mixtures of fluopyram + trifloxystrobin and pyraclostrobin + boscalid, performed better than contact and penetrant products and showed up to 90% control against RLB. However, the efficacy of the tested fungicides varied depending on the year. In terms of application strategies, when fungicide applications were conducted following specific meteorological conditions (after 15 days from >15 mm rainfalls with ≈10–15 ◦C as the minimum average temperature), their efficacy was comparable to that of calendar-based treatments (every 14, 21, or 31 days from petal fall) but with fewer applications (depending on the year, 2–4 applications as compared with 5–9 for calendar treatments)info:eu-repo/semantics/publishedVersio

Almond Fruit Drop Patterns under Mediterranean Conditions

Almond is an important tree nut crop worldwide, and planted areas have been increasing year after year. While self-fertility is one of the key factors when it comes to improved almond productivity of new cultivars, yield is also affected by the number of flowers produced, pollination, fruit set, fruit drop, and fruit weight. Almond fruit drop patterns of 20 Mediterranean almond cultivars were studied over three years. In addition, fruit drop patterns of two scion cultivars ‘Marinada’ and ‘Vairo’ budded onto eight to 10 different rootstocks managed with three different pruning strategies were studied for two years. Cumulative flower and fruit drop ranged from 50% to 90% among cultivars and treatments, and there were up to four fruit drop events during the growing season, the main one occurring from 20–60 days from full bloom (DFFB). Subsequent drops were at 100 DFFB, 120–140 DFFB, and the last one at 160–180 DFFB. The later drops were less apparent. In general, about half of the cumulative drop was comprised of buds and flowers, and the remaining percentage was fruit that dropped 20 or more days after full bloom. Furthermore, different fruit drop patterns were observed depending on the cultivar. For late- and extra-late flowering cultivars, cumulative fruit drop began to decrease earlier, with most of the drops occurred already at full bloom, whereas the opposite was observed for the early flowering cultivars. Rootstocks also had an important effect on the fruit drop pattern, with different effects depending on the scion cultivar. Tree management, such as type of pruning, also had an important effect on the rate of fruit drop and cumulative drop. Therefore, each combination of cultivar × rootstock × pruning type will require different strategies in order to reduce the fruit drop and optimize crop loads.info:eu-repo/semantics/publishedVersio

Airborne inoculum dynamics of Polystigma amygdalinum and progression of almond red leaf blotch disease in Catalonia, NE Spain

The dynamics of airborne ascospores and disease progress of red leaf blotch (RLB) of almond, caused by Polystigma amygdalinum, and their correlations with weather variables were studied from 2019 to 2021 in two almond orchards located in Lleida, NE Spain. Airborne ascospores were detected and quantified by real-time qPCR using species-specific primers for P. amygdalinum. Ascospores were detected mainly from April to June, with a high variability between the yearly cumulative concentrations. Positive significant correlations were found between the weekly proportion of airborne ascospores and the number of wet and mild days—either combined or separated— accumulated rainfall, number of rainy days, accumulated low temperatures on wet days, and mean and maximum relative humidity. In contrast, several thermal variables (maximum temperature, VPD, and number of warm days) were negatively correlated with ascospore catches. Positive significant correlations were found between the cumulative proportion of ascospores and RLB incidence and severity. Weekly variations in RLB incidence and severity showed significant positive correlations with the number of warm days while negative with the number of mild days. Severity was also positively correlated with several thermal variables (mean, maximum, and minimum temperature, and VPD), and negatively correlated with the number of cold days and wet and mild days. Stronger correlations were generally found with ascospore catches or disease progress when using concurrent weekly weather data. Gompertz, monomolecular, and logistic growth models were evaluated to describe RLB disease progressinfo:eu-repo/semantics/publishedVersio

Susceptibility of Almond (Prunus dulcis) Cultivars to Twig Canker and Shoot Blight Caused by Diaporthe amygdali

Twenty-five almond cultivars were assessed for susceptibility to Diaporthe amygdali, causal agent of twig canker and shoot blight disease. In laboratory experiments, growing twigs were inoculated with four D. amygdali isolates. Moreover, growing shoots of almond cultivars grafted onto INRA ‘GF-677’ rootstock were used in 4-year field inoculations with one D. amygdali isolate. In both types of experiments, inoculum consisted of agar plugs with mycelium, which were inserted underneath the bark, and the lesion lengths caused by the fungus were measured. Necrotic lesions were observed in the inoculated almond cultivars in both laboratory and field tests, confirming the susceptibility of all evaluated cultivars to all inoculated isolates of D. amygdali. Cultivars were grouped as susceptible or very susceptible according to a cluster analysis. The relationship between some agronomic traits and cultivar susceptibility was also investigated. Blooming and ripening times were found to be relevant variables explaining cultivar performance related to D. amygdali susceptibility. Late and very late blooming and early and medium ripening cultivars were highly susceptible to D. amygdali. Our results may provide valuable information that could assist in ongoing breeding programs of this crop and in the selection of cultivars for new almond plantations.info:eu-repo/semantics/acceptedVersio

Inoculum and infection dynamics of Polystigma amygdalinum in almond orchards in Spain

Red leaf blotch (RLB) disease of almond, caused by Polystigma amygdalinum, is an important foliar disease in most production regions of the Mediterranean basin and the Middle East because severe infections may cause a premature defoliation of the tree. Some key aspects on the epidemiology of P. amygdalinum were studied in multiyear trials in two almond-growing regions in Spain, which included the seasonal development of perithecia and production and germination of ascospores along with the disease incubation and plant infectivity periods. Our results showed that primary inoculum was available in extended periods (January to August). Significant differences in ascospore amounts among regions, higher in the southern Andalusia and lower in the northern Catalonia, and years of study were detected. The factors geographical location, sampling period, and evaluation year were found significant on the development of P. amygdalinum perithecia. Variable ascospore germination rates were observed from April to July: >15% but rarely exceeding 30%. The RLB infectivity period in Catalonia extended from March to mid-June, whereas in Andalusia it was from March to May. The incubation period was mainly in a range of 5 to 10 weeks in Catalonia. The environmental conditions of October to January influence the available ascospore amounts in the next season. RLB infection occurs in spring to summer when mean temperatures are in the range 10 to 20°C. These results represent the first step in developing a prediction model of the disease that might serve as a tool for the control of RLB.info:eu-repo/semantics/acceptedVersio

Modelización del inóculo aéreo de Polystigma amygdalinum para optimizar el control de la mancha ocre del almendro

La mancha ocre del almendro, causada por el ascomiceto Polystigma amygdalinum, es una de las enfermedades foliares más importantes de este cultivo en la cuenca mediterránea. El patógeno pasa el invierno en la hojarasca, donde maduran peritecios y ascosporas. Las ascosporas se liberan en primavera, dando lugar a nuevas infecciones. La principal estrategia de control de esta enfermedad consiste en la aplicación periódica de fungicidas. El objetivo de este trabajo consistió en desarrollar un modelo matemático para predecir la liberación de ascosporas a lo largo de la temporada del cultivo

Survey of Oomycetes Associated with Root and Crown Rot of Almond in Spain and Pathogenicity of Phytophthora niederhauserii and Phytopythium vexans to ‘Garnem’ Rootstock

From 2018 to 2020, surveys of oomycetes associated with root and crown rot of almond (Prunus dulcis) were conducted on diseased young almond trees in commercial orchards and nurseries in six provinces of Spain. A total of 104 oomycete isolates were obtained from plant and soil samples, which h were identified by sequencing the internal transcribed spacer (ITS) region of the ribosomal DNA. Diverse species belonging to the genera Globisporangium, Phytophthora, Phytopythium and Pythium were found, Phytopythium vexans and Phytophthora niederhauserii being the most frequent. The pathogenicity of these two species to one-year-old almond seedlings of ‘Garnem’ (P. dulcis × P. persica) rootstock was studied. All seedlings inoculated with Pp. vexans and Ph. niederhauserii isolates showed severe symptoms at the late stage of the pathogenicity test (defoliation, wilting and dieback) and several plants died. Some isolates of Ph. niederhauserii significantly reduced the dry weight of the roots compared with the control, but this effect was not observed in seedlings inoculated with Pp. vexans. These results provide new information about the oomycete species present in almond crops in Spain and highlight the importance of carrying out frequent phytosanitary surveys for a better knowledge of potential risks posed by these soil-borne pathogens.info:eu-repo/semantics/publishedVersio

Horticultural performance of ‘Marinada’ and ‘Vairo’ almond cultivars grown on a genetically diverse set of rootstocks

Evolution of almond planted area and production has been mainly due to the arrival of new cultivars and rootstocks that have contributed to improve agronomic characters such as yield, precocity and efficiency. In recent years, new cultivars that have contributed to provide late blooming time and self-fertility, and with ease to adapt for mechanical harvest and high-density are becoming available. However, there is scarcity of studies where the interaction of these new cultivars with hybrid rootstocks has been tested. The aim of this study was to assess the performance of Cadaman®, Garnem®, INRA GF-677, IRTA-1, IRTA-2, Ishtara®, Adesoto, Rootpac® 20, Rootpac® 40, and Rootpac® R rootstocks with two promising almond cultivars such as ‘Marinada’ and ‘Vairo’. Bloom and nut ripening dates were affected by rootstock genotype. Both ‘Marinada’ and ‘Vairo’ cultivars showed low biennial bearing, with some differences among rootstocks, with IRTA-2 and Adesoto inducing the lowest values. On the other hand, Adesoto had higher number of suckers than the rest of the rootstocks. Garnem® provided the biggest trees, followed by Cadaman®, and then a third group which comprised IRTA-2 and INRA GF-677. Rootpac® 20 was the most dwarfing rootstock, followed by IRTA-1, Adesoto, Ishtara®, Rootpac® R, and Rootpac® 40. In terms of yield efficiency and partitioning index, IRTA-1, INRA GF-677, and Rootpac® R were the ones with higher values. Differences in tree volume and vigor for these rootstocks suggested that INRA GF-677 would be a suitable rootstock for low-medium planting densities with wide spacings; whereas Rootpac® R and IRTA-1 would be suitable rootstocks for medium- and high-density plantings. Findings of this study showed dramatic differences in tree vigor, yield, kernel weight, yield efficiency, and partitioning index, which provide a wide range of options to deem for each cultivar in a particular climate and management.info:eu-repo/semantics/acceptedVersio