Identification of Vascular and Hematopoietic Genes Downstream of etsrp by Deep Sequencing in Zebrafish

The transcription factor etsrp/Er71/Etv2 is a master control gene for vasculogenesis in all species studied to date. It is also required for hematopoiesis in zebrafish and mice. Several novel genes expressed in vasculature have been identified through transcriptional profiling of zebrafish embryos overexpressing etsrp by microarrays. Here we re-examined this transcriptional profile by Illumina RNA-sequencing technology, revealing a substantially increased number of candidate genes regulated by etsrp. Expression studies of 50 selected candidate genes from this dataset resulted in the identification of 39 new genes that are expressed in vascular cells. Regulation of these genes by etsrp was confirmed by their ectopic induction in etsrp overexpressing and decreased expression in etsrp deficient embryos. Our studies demonstrate the effectiveness of the RNA-sequencing technology to identify biologically relevant genes in zebrfish and produced a comprehensive profile of genes previously unexplored in vascular endothelial cell biology

Tumour vascularization: sprouting angiogenesis and beyond

Tumour angiogenesis is a fast growing domain in tumour biology. Many growth factors and mechanisms have been unravelled. For almost 30 years, the sprouting of new vessels out of existing ones was considered as an exclusive way of tumour vascularisation. However, over the last years several additional mechanisms have been identified. With the discovery of the contribution of intussusceptive angiogenesis, recruitment of endothelial progenitor cells, vessel co-option, vasculogenic mimicry and lymphangiogenesis to tumour growth, anti-tumour targeting strategies will be more complex than initially thought. This review highlights these processes and intervention as a potential application in cancer therapy. It is concluded that future anti-vascular therapies might be most beneficial when based on multimodal anti-angiogenic, anti-vasculogenic mimicry and anti-lymphangiogenic strategies

The temperature of a brake disc during frictional heating with linear distribution of the contact pressure

In this paper the analytical solution of the boundary–value heat conduction problem for a brake rotor was developed. A solid brake disc is heated by frictional heat flux during braking with constant deceleration. Intensity of the heat flux affecting friction surface of the disc is proportional to the specific power of friction. It was assumed that contact pressure between the pad and the disc increases linearly, from zero in the initial moment of the braking process to the maximum value in standstill. Calculations were carried out on variables and parameters in the dimensionless form. The obtained results were compared with adequate resultsduring braking with constant deceleration, with an assumption of pressure constant in time

Temperature distribution in a brake disc with variable contact pressure

The aim of this study was to investigate the influence of the time of pressure increase during single braking on the temperature in a brake disc. The case of linear pressure increase from zero to nominal value in the initial stage of braking and maintaining this value to standstill was considered. The time distribution of the sliding velocity of frictional elements was determined from the differential equation of motion with the initial condition. Based on the time distributions of pressure and sliding velocity, the intensity of the frictional heat flux, which affects on the disc surface, was determined. Spatio-temporal distribution of the temperature in a brake disc was found from analytical solution of the heat conduction boundary–value problem for semi–space heated on the outer surface heat flux with known a priori intensity. The numerical analysis conducted allowed to determine engineering equation, which describes relation between maximum temperature and the time of pressure increase

Analytical model for investigation of the effect of friction power on thermal stresses in friction elements of brakes

The influence of the change with time of friction power on thermal stresses in a friction element of a brake system is investigated. For this purpose, a list of ten different temporal profiles of specific friction power is used, which has been experimentally established for a single braking process. The corresponding profiles of transient temperature fields in the considered element are applied as input parameters to find quasi-static thermal stresses. Numerical analysis concerned with spatio-temporal distributions of the stresses and their evolutions on the heated surface is presented