10 th Annual Conference of the International FES Society

Abstract This was a randomized pilot study in which FES was applied as a therapeutic tool to help patients improve voluntary grasp and release movements. The goal of the treatment was to increase hand function, independence in activities of daily living and quality of life. In the treatment group (N=13), daily FES was applied to stimulate weak and non-functional muscles in a sequence of movements to coordinate a hand grasp for manipulating everyday objects. In the control group (N=9), patients received conventional occupational therapy of equal intensity to the treatment group. The following tests were used to measure change: Rehabilitation Engineering Laboratory Hand Function Test, Functional Independence Measure, Spinal Cord Independence Measure and a Qualitative Interview. Preliminary results suggest: 1) the proposed treatment has a positive impact on voluntary hand grasp when used in combination with occupational therapy; 2) greater improvement was expected with incomplete participants, but was also seen with our patients with complete injuries; 3) stimulation programs need to be adjusted by an occupational therapist regularly; and 4) patients report improved voluntary hand function, greater independence and quality of life as a result of the neuroprosthesis treatment

Pancreatic preneoplastic lesions plasma signatures and biomarkers based on proteome profiling of mouse models

International audienc

Identification of novel biomarker and therapeutic target candidates for acute intracerebral hemorrhage by quantitative plasma proteomics

Abstract Background The systematic mechanisms of acute intracerebral hemorrhage are still unknown and unverified, although many recent researches have indicated the secondary insults. This study was aimed to disclose the pathological mechanism and identify novel biomarker and therapeutic target candidates by plasma proteome. Methods Patients with AICH (n = 8) who demographically matched healthy controls (n = 4) were prospectively enrolled, and their plasma samples were obtained. The TMT-LC–MS/MS-based proteomics approach was used to quantify the differential proteome across plasma samples, and the results were analyzed by Ingenuity Pathway Analysis to explore canonical pathways and the relationship involved in the uploaded data. Results Compared with healthy controls, there were 31 differentially expressed proteins in the ICH group (P < 0.05), of which 21 proteins increased while 10 proteins decreased in abundance. These proteins are involved in 21 canonical pathways. One network with high confidence level was selected by the function network analysis, in which 23 proteins, P38MAPK and NFκB signaling pathways participated. Upstream regulator analysis found two regulators, IL6 and TNF, with an activation z-score. Seven biomarker candidates: APCS, FGB, LBP, MGMT, IGFBP2, LYZ, and APOA4 were found. Six candidate proteins were selected to assess the validity of the results by subsequent Western blotting analysis. Conclusion Our analysis provided several intriguing pathways involved in ICH, like LXR/RXR activation, acute phase response signaling, and production of NO and ROS in macrophages pathways. The three upstream regulators: IL-6, TNF, LPS, and seven biomarker candidates: APCS, APOA4, FGB, IGFBP2, LBP, LYZ, and MGMT were uncovered. LPS, APOA4, IGFBP2, LBP, LYZ, and MGMT are novel potential biomarkers in ICH development. The identified proteins and pathways provide new perspectives to the potential pathological mechanism and therapeutic targets underlying ICH