The experimental study of the pathogenic role of NADPH-oxidase in the thyroid gland tissues

The objective of the study was to compare the NADPH/ H2O2 system and the expression of enzymes in the culture of thyroid gland cells, depending on the sex. Material and methods. The thyroid gland was collected from adult female donors (n=20) and men (n=15). The cells were washed twice with HBSS and centrifuged at 1000 × g for 5 minutes, and then cultured to 106 cells in 0.5 ml culture medium per well in 24-well plates (Thermo Fisher Scientific, USA). H2O2 levels in the cells were determined quantitatively using a hydrogen peroxide sensor Premo™ Orp1-roGFP (Life Technologies, USA) according to manufacturer’s instructions. Statistical analysis of the results was carried out using Statistica 6.1 (StatSoft, Inc., USA). Results. We found that the expression of DUOX1 and NOX4 in the thyroid gland was significantly higher in women than in men (1.5 times, p<0.05). Expression of DUOX2 in female cells was 1.5 times higher than in men, but statistically significant (p=0.06). We have found that the thyroid gland of women produces a significantly higher H2O2 content than the male gland cells (p<0.05). The expression of DUOX1 and NOX4 is significantly higher in cells derived from the female thyroid gland than in cells derived from males. H2O2 levels were also higher in cells derived from female thyroid gland than in male cells. Conclusion. As a result of our experiment, the expression of DUOX1 and NOX4 is significantly higher in cells derived from the female thyroid gland than in cells derived from males. We have shown during the experiment that NOX4 is regulated both at the transcription level and at the level after transcription, and the level of sustained-release NOX4 mRNA does not accurately reflect the content and function of the NOX4 protein. Chronic effects on the female thyroid gland at higher concentrations of H2O2 can potentially cause many adverse effects

Влияние релаксационной прочности зерен и межзеренных границ в котельных сталях на работоспособность поверхностей нагрева

Представлены экспериментальные результаты исследований отечественной разработки аустенитной хромомарганцевой стали 10Х13Г12С2Н2Д2Б (Ди-59). На основе рентгенометрии показано, что осцилляции внутренних напряжений при циклическом изменении температуры, сопровождающиеся процессами релаксации при перемене знака, окажут существенное влияние на прочностные характеристики и послужат причиной термической усталости металла, а критерии релаксации напряжений и условия их протекания станут определяющими для работы металла при высоких температурах эксплуатации. Обоснованный выбор температуры эксплуатации, существенно повышающий релаксационную прочность зерен и межзеренных границ сплава, является основой продления ресурса работоспособности

Different tissue reaction of oesophagus and diaphragm after mesh hiatoplasty. Results of an animal study

<p>Abstract</p> <p>Background</p> <p>Laparoscopic mesh-reinforcement of the hiatal region in the treatment of gastroesophageal reflux disease (GERD) and paraesophageal hernia (PEH) reduces the risk of recurrence. However, there are still controversies about the technique of mesh placement, shape, structure and material. We therefore compared tissue integration and scar formation after implantation of two different polypropylene-meshes in a rabbit model.</p> <p>Methods</p> <p>A total of 20 female chinchilla rabbits were included in this study. Two different meshes (Polypropylene PP, Polyglecaprone 25 Composite PP-PG) were implanted on the abdominal diaphragm around the oesophagus. After 3 months the implanted meshes were excised en-bloc. Histological and morphological analyses were carried out accordingly proliferation rate, apoptosis and collagen type I/III ratio.</p> <p>Results</p> <p>Regarding proliferation rate of oesophagus PP (9.31 ± 3.4%) and PP-PG (13.26 ± 2.54%) differ in a significant (p = 0.0097) way. In the diaphragm we found a significant (p = 0.00066) difference between PP (9.43 ± 1.45%) and PP-PG (18.73 ± 5.92%) respectively. Comparing oesophagus and diaphragm we could prove a significant difference within PP-PG-group (p = 0.0195). Within PP-group the difference reached no statistical significance (p = 0.88). We found analogous results regarding apoptosis.</p> <p>Furthermore, there is a significant (p = 0.00013) difference of collagen type I/III ratio in PP-PG (12.28 ± 0.8) compared to PP (8.44 ± 1,63) in case of oesophageal tissue. Concerning diaphragm we found a significant difference (p = 0.000099) between PP-PG (8.85 ± 0.81) and PP (6.32 ± 1.07) as well.</p> <p>Conclusion</p> <p>The histologic and morphologic characteristics after prosthetic enforcement of the hiatus in this animal model show a more distinct tissue integration using PP-PG compared to PP. Additionally, different wound healing and remodelling capability influence tissue integration of the mesh in diaphragm and oesophagus.</p

The influence of blood on the efficacy of intraperitoneally applied phospholipids for prevention of adhesions

<p>Abstract</p> <p>Background</p> <p>The formation of adhesions following abdominal surgery is a well known problem. In previous studies we demonstrated the efficacy and safety of intraperitoneally applied phospholipids in order to prevent adhesion formation. This study evaluates the influence of blood on the efficacy of intraperitoneally applied phospholipids for prevention of adhesions.</p> <p>Methods</p> <p>In 40 Chinchilla rabbits adhesions were induced by median laparotomy, standardized abrasion of the visceral and parietal peritoneum in defined areas of the ventral abdominal wall and the caecum. The animals were randomly divided into four groups. They received either phospholipids 3.0% or normal saline (NaCl 0,9%) (5 ml/kg body weight). In 50% of the rabbits we simulated intraperitoneal bleeding by administration of blood (1,5 ml/kg body weight). The other half served as control group. Ten days following the operation the animals were sacrificed and adhesion formation was assessed by computer aided planimetry and histopathologic examination.</p> <p>Results</p> <p>The median adhesion surface area in the NaCl-group (n = 9) amounted to 68,72 mm², in the NaCl+Blood-group (n = 10) 147,68 mm². In the Phospholipid (PhL)-group (n = 9) the median adhesion surface area measured 9,35 mm², in the PhL+Blood-group (n = 9) 11,95 mm². The phospholipid groups had a significantly smaller adhesion surface area (p < 0.05).</p> <p>Conclusion</p> <p>Again these results confirm the efficacy of phospholipids in the prevention of adhesions in comparison to NaCl (p = 0,04). We also demonstrated the adhesion preventing effect of phospholipids in the presence of intraperitoneal blood.</p

За кадры. 1936. № 4-5

"Клянемся тебе товарищ Ленин..."Горный орел / И. В. СталинЛенин умер, но дело его живет / Н. К. КрупскаяО жизни и деятельности В. И. Ленина. В гимназииО жизни и деятельности В. И. Ленина. Казнь братаО жизни и деятельности В. И. Ленина. Университет и арест / А. И. Елизарова-УльяноваО жизни и деятельности В. И. Ленина. Рабочие кружки. Тюрьма. Ссылка / Н. К. Крупская, А. И. Елизарова-УльяноваО жизни и деятельности В. И. Ленина. Революция 1905 г. / А. И. Елизарова-УльяноваО жизни и деятельности В. И. Ленина. Февраль 1917 г. / А. И. Елизарова-УльяноваО жизни и деятельности В. И. Ленина. Встреча у финляндского вокзалаО жизни и деятельности В. И. Ленина. С Лениным-против войны / ЕгоровО жизни и деятельности В. И. Ленина. Октябрьская революция / Н. К. КрупскаяО жизни и деятельности В. И. Ленина. Покушение на Ленина / Н. ИвановО жизни и деятельности В. И. Ленина. Встреча на Путиловском заводе / ДаниловО жизни и деятельности В. И. Ленина. В жизнь мою въелись его слова / КорягинО жизни и деятельности В. И. Ленина. Рассказ маляра о встрече с Лениным на субботнике / С. Д. НаумовО жизни и деятельности В. И. Ленина. О пуске металлического завода / ЛажечниковО жизни и деятельности В. И. Ленина. Ленин на крестьянской сходке / А. С. ГубанцевО жизни и деятельности В. И. Ленина. Ильич больнойО жизни и деятельности В. И. Ленина. Над гробом / Д. А. СоколоваО жизни и деятельности В. И. Ленина. Сталин продолжатель дела Ленина / ГладышевЧто такое ленинизм / И. В. Сталин21 января / Л. Длигач"Кровавое воскресенье"Ко всем рабочим9 января / М. ГорькийРезультат плохой работыПервые дни сессии по энергофаку / Н.Защита дипломных проектов и дипломных работ на химико-технологическом факультете / И. ПономаревПереработка типовых программ на специальности "Геологическая съемка и поиски" / М. А. УсовПересмотр программы закончен / Д. А. СтрельниковДержать связь с производством / БелослудцевС блокнотом по институтуКниги детям колхозников / Н. ОхотниковаО перестройке профработы на ОТФ / Г. КоломеецПрошу совета / Поспело