Genome-wide transcriptome analysis of gametophyte development in Physcomitrella patens

<p>Abstract</p> <p>Background</p> <p>Regulation of gene expression plays a pivotal role in controlling the development of multicellular plants. To explore the molecular mechanism of plant developmental-stage transition and cell-fate determination, a genome-wide analysis was undertaken of sequential developmental time-points and individual tissue types in the model moss <it>Physcomitrella patens </it>because of the short life cycle and relative structural simplicity of this plant.</p> <p>Results</p> <p>Gene expression was analyzed by digital gene expression tag profiling of samples taken from <it>P. patens </it>protonema at 3, 14 and 24 days, and from leafy shoot tissues at 30 days, after protoplast isolation, and from 14-day-old caulonemal and chloronemal tissues. In total, 4333 genes were identified as differentially displayed. Among these genes, 4129 were developmental-stage specific and 423 were preferentially expressed in either chloronemal or caulonemal tissues. Most of the differentially displayed genes were assigned to functions in organic substance and energy metabolism or macromolecule biosynthetic and catabolic processes based on gene ontology descriptions. In addition, some regulatory genes identified as candidates might be involved in controlling the developmental-stage transition and cell differentiation, namely MYB-like, HB-8, AL3, zinc finger family proteins, bHLH superfamily, GATA superfamily, GATA and bZIP transcription factors, protein kinases, genes related to protein/amino acid methylation, and auxin, ethylene, and cytokinin signaling pathways.</p> <p>Conclusions</p> <p>These genes that show highly dynamic changes in expression during development in <it>P. patens </it>are potential targets for further functional characterization and evolutionary developmental biology studies.</p

A Highly Sensitive Intensity-Modulated Optical Fiber Magnetic Field Sensor Based on the Magnetic Fluid and Multimode Interference

Fiber-optic magnetic field sensing is an important method of magnetic field monitoring, which is essential for the safety of civil infrastructures, especially for power plant. We theoretically and experimentally demonstrated an optical fiber magnetic field sensor based on a single-mode-multimode-single-mode (SMS) structure immersed into the magnetic fluid (MF). The length of multimode section fiber is determined based on the self-image effect through the simulation. Due to variation characteristics of the refractive index and absorption coefficient of MF under different magnetic fields, an effective method to improve the sensitivity of SMS fiber structure is realized based on the intensity modulation method. This sensor shows a high sensitivity up to 0.097 dB/Oe and a high modulation depth up to 78% in a relatively linear range, for the no-core fiber (NCF) with the diameter of 125 μm and length of 59.8 mm as the multimode section. This optical fiber sensor possesses advantages of low cost, ease of fabrication, high sensitivity, simple structure, and compact size, with great potential applications in measuring the magnetic field

Carbon-coated magnetic particles increase tissue temperatures after laser irradiation

Purpose: This work focused on the investigation the hyperthermia performance of the carbon-coated magnetic particles (CCMPs) in laser-induced hyperthermia. Materials and methods: We prepared CCMPs using the organic carbonization method, and then characterized them with transmission electron microscopy (TEM), ultraviolet-visible (UV-Vis) spectrophotometry, vibrating sample magnetometer (VSM) and X-ray diffraction (XRD). In order to evaluate their performance in hyperthermia, the CCMPs were tested in laser-induced thermal therapy (LITT) experiments, in which we employed a fully distributed fiber Bragg grating (FBG) sensor to profile the tissue's dynamic temperature change under laser irradiation in real time. Results: The sizes of prepared CCMPs were about several micrometers, and the LITT results show that the tissue injected with the CCMPs absorbed more laser energy, and its temperature increased faster than the contrast tissue without CCMPs. Conclusions: The CCMPs may be of great help in hyperthermia applications

No Detectable Maternal Effects of Elevated CO2 on Arabidopsis thaliana Over 15 Generations

Maternal environment has been demonstrated to produce considerable impact on offspring growth. However, few studies have been carried out to investigate multi-generational maternal effects of elevated CO2 on plant growth and development. Here we present the first report on the responses of plant reproductive, photosynthetic, and cellular characteristics to elevated CO2 over 15 generations using Arabidopsis thaliana as a model system. We found that within an individual generation, elevated CO2 significantly advanced plant flowering, increased photosynthetic rate, increased the size and number of starch grains per chloroplast, reduced stomatal density, stomatal conductance, and transpiration rate, and resulted in a higher reproductive mass. Elevated CO2 did not significantly influence silique length and number of seeds per silique. Across 15 generations grown at elevated CO2 concentrations, however, there were no significant differences in these traits. In addition, a reciprocal sowing experiment demonstrated that elevated CO2 did not produce detectable maternal effects on the offspring after fifteen generations. Taken together, these results suggested that the maternal effects of elevated CO2 failed to extend to the offspring due to the potential lack of genetic variation for CO2 responsiveness, and future plants may not evolve specific adaptations to elevated CO2 concentrations

Structure of photosystem I-LHCI-LHCII from the green alga Chlamydomonas reinhardtii in State 2

Photosystem I (PSI) and II (PSII) balance their light energy distribution absorbed by their light-harvesting complexes (LHCs) through state transition to maintain the maximum photosynthetic performance and to avoid photodamage. In state 2, a part of LHCII moves to PSI, forming a PSI-LHCI-LHCII supercomplex. The green alga Chlamydomonas reinhardtii exhibits state transition to a far larger extent than higher plants. Here we report the cryo-electron microscopy structure of a PSI-LHCI-LHCII supercomplex in state 2 from C. reinhardtii at 3.42 Å resolution. The result reveals that the PSI-LHCI-LHCII of C. reinhardtii binds two LHCII trimers in addition to ten LHCI subunits. The PSI core subunits PsaO and PsaH, which were missed or not well-resolved in previous Cr-PSI-LHCI structures, are observed. The present results reveal the organization and assembly of PSI core subunits, LHCI and LHCII, pigment arrangement, and possible pathways of energy transfer from peripheral antennae to the PSI core

Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea

Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin chlorophyll a/c-binding proteins (ACPs) to act as the peripheral light-harvesting system, whose supramolecular organization is unknown. Here, we purify the PSII-ACPII supercomplex from a cryptophyte alga Chroomonas placoidea (C. placoidea), and analyze its structure at a resolution of 2.47 & Aring; using cryo-electron microscopy. This structure reveals a dimeric organization of PSII-ACPII containing two PSII core monomers flanked by six symmetrically arranged ACPII subunits. The PSII core is conserved whereas the organization of ACPII subunits exhibits a distinct pattern, different from those observed so far in PSII of other algae and higher plants. Furthermore, we find a Chl a-binding antenna subunit, CCPII-S, which mediates interaction of ACPII with the PSII core. These results provide a structural basis for the assembly of antennas within the supercomplex and possible excitation energy transfer pathways in cryptophyte algal PSII, shedding light on the diversity of supramolecular organization of photosynthetic machinery

Over 100 mW stable low-noise single-frequency ring-cavity fiber laser based on a saturable absorber of Bi/Er/Yb co-doped silica fiber

Two kinds of Yb-doped fibers were fabricated, namely, Yb: YAG crystal-derived silica fibers (YCDSFs) with a gain coefficient of 6.0 dB/cm, and Bi/Er/Yb co-doped silica fibers having a Yb concentration of 0.1310^26 ion/m^3. Based on these fibers, a ring-cavity single-frequency fiber laser (SFFL) has been constructed, in which the YCDSF was used as a gain medium and the Bi/Er/Yb co-doped fiber acted as a saturable absorber. It has been demonstrated that the SFFL had an over 100 mW output at 1030 nm, slope-efficiency of up to 18.3%, and an optical signal-to-noise ratio of over 63 dB. The fluctuation of the output power of the laser was less than 0.65% of 103.5 mW within 10 hrs and no mode-hopping was observed for 5 hrs. The SFFL had a linewidth <7.5 kHz at the maximum output power, and the measured relative intensity noise was lower than 142 dB/Hz at a frequency above 1.0 MHz. The results indicate that the ring-cavity SFFL built could be used as a laser source for applications in a high-power fiber laser, and high-precision optical fiber sensing and detection

Antenna arrangement and energy-transfer pathways of PSI-LHCI from the moss Physcomitrella patens

Plants harvest light energy utilized for photosynthesis by light-harvesting complex I and II (LHCI and LHCII) surrounding photosystem I and II (PSI and PSII), respectively. During the evolution of green plants, moss is at an evolutionarily intermediate position from aquatic photosynthetic organisms to land plants, being the first photosynthetic organisms that landed. Here, we report the structure of the PSI-LHCI supercomplex from the moss Physcomitrella patens (Pp) at 3.23 angstrom resolution solved by cryo-electron microscopy. Our structure revealed that four Lhca subunits are associated with the PSI core in an order of Lhca1-Lhca5-Lhca2-Lhca3. This number is much decreased from 8 to 10, the number of subunits in most green algal PSI-LHCI, but the same as those of land plants. Although Pp PSI-LHCI has a similar structure as PSI-LHCI of land plants, it has Lhca5, instead of Lhca4, in the second position of Lhca, and several differences were found in the arrangement of chlorophylls among green algal, moss, and land plant PSI-LHCI. One chlorophyll, PsaF-Chl 305, which is found in the moss PSI-LHCI, is located at the gap region between the two middle Lhca subunits and the PSI core, and therefore may make the excitation energy transfer from LHCI to the core more efficient than that of land plants. On the other hand, energy-transfer paths at the two side Lhca subunits are relatively conserved. These results provide a structural basis for unravelling the mechanisms of light-energy harvesting and transfer in the moss PSI-LHCI, as well as important clues on the changes of PSI-LHCI after landing