GPU Accelerated High Intensity Ultrasound Acoustical Power Computation

International audienceThe simulation of the hepatocellular carcinoma therapy effects is often used for the intervention planning. As the physical-based model of the simulation is very time-consuming, the speed of this method becomes an obstacle during the clinical application simulation. In order to accelerate the simulation, a GPU-based (Graphic Processing Unit) acceleration method of the pressure field estimation is proposed in this paper. The results demonstrate that the proposed acceleration method can solve the time-consuming problem

Induction of the mesenchymal to epithelial transition by demethylation-activated microRNA-125b is involved in the anti-migration/invasion effects of arsenic trioxide on human chondrosarcoma

Background: In addition to treating acute promyelocytic leukemia, arsenic trioxide (ATO) suppresses other solid tumors, including chondrosarcoma. However, the effects of ATO on metastasis in chondrosarcoma cells, and the underlying molecular mechanisms remain unclear. Methods: The effects of ATO on the migratory and invasive capacities of chondrosarcoma cells were investigated by Wound healing, Transwell and EMT assays. The expression of miR-125b in human chondrosarcoma tissues and cell lines was detected by real-time PCR analysis. Bisulfite sequencing analysis (BSP) was used to detect the effects of ATO on the expression of miR-125b. The gain-of-function and loss-of-function experiments were performed on chondrosarcoma cell lines to investigate the effects of miR-125b on chondrosarcoma invasion, and to determine whether signal transducer and activator of transcription 3(Stat3) mediates these effects. Dual-luciferase reporter assay was used to identify whether Stat3 is a direct target of miR-125b. Results: MiR-125b was significantly downregulated in human metastatic chondrosarcoma tissues and cell lines but not in non-metastatic chondrosarcoma tissues. ATO up-regulates the expression of miR-125b by the demethylation of DNA. ATO induces MET and attenuates the invasive capacities of chondrosarcoma cells through miR-125b. Stat3 was verified as a direct target of miR-125b, which is involved in ATO regulating EMT-associated traits. Conclusions: These findings, for the first time, provides evidence that the miR-125b-mediated inhibition of Stat3 is involved in the ATO-induced attenuation of metastasis in chondrosarcoma cells.National Natural Science Foundation of China [81572633]; Research Fund for the Doctoral Program of Higher Education of China [20130001110076]SCI(E)[email protected]

Metal chalcogenide hollow polar bipyramid prisms as efficient sulfur hosts for Na-S batteries

Funding Information: We appreciate the support from the National Natural Science Foundation of China (Nos. 21773188 and 21972111), Fundamental Research Funds for the Central Universities (XDJK2019AA002), Postgraduate tutor team-building project (XYDS201911). The work at UT was supported by the Welch Foundation (F-1841) and the Texas Advanced Computing Center.Peer reviewe

Sexual dimorphic effects of igf1 deficiency on metabolism in zebrafish

Insulin-like growth factor 1 (IGF1) is an essential effector of the growth hormone (GH)/IGF1 axis for somatic growth regulation in mammals. However, its functions have not been thoroughly investigated in zebrafish in vivo. In this study, the igf1-deficient zebrafish model was developed using the CRISPR/Cas9 technique. In this study all the results were performed on both male and female animals. The growth of both male and female igf1-deficient zebrafish were reduced. The igf1 deficiency leads to significant complementary up-regulation of transcriptional expression levels of insulin, igf2 and igf3. This suggested that igf2 and igf3 may act with redundant functions. While the upregulation of gh1 expression can only be detected in igf1-deficient females. At the same time, significant growth retardation, fatty liver, reduced activated levels of ribosomal S6 (S6) are seen only in igf1-deficient males. On the other hand, significant hyperglycemia, elevated transcriptional expression levels of phosphenolpyruvate carboxykinase (pepck) and levels of phosphorylated extracellular signal-regulated kinase (ERK1/2), with additional reduced hepatic lactate/pyruvate (L/P) ratios can only observed in igf1-deficient females. Impaired glucose uptake has been recorded in the primary cultured hepatocytes from igf1-deficient females, but not males. Intriguingly, exposure to 17beta-estroadiol (E2) can partially ameliorated the defects of fatty liver and activation of AKT/mTOR signaling in igf1-deficient males. Our studies demonstrate the significant functions of IGF1 on somatic regulation in zebrafish, with asymmetric gender-related consequences. Our data thus suggest that the zebrafish IGF1 is preferentially required for the activation of AKT/mTOR signaling in male zebrafish, but glucose uptake in females

Horizontally Acquired Polysaccharide-Synthetic Gene Cluster From Weissella cibaria Boosts the Probiotic Property of Lactiplantibacillus plantarum

Lactiplantibacillus plantarum are probiotic bacteria, maintaining the integrity of the gastrointestinal epithelial barrier, and preventing the infection of pathogenic bacteria. Exopolysaccharides (EPSs) are often involved in the probiotic property of L. plantarum. Here, we identified a new EPS-synthetic gene cluster, cpsWc, carrying 13 genes, laid on a large plasmid in a well-characterized probiotic L. plantarum strain LTC-113. The cpsWc gene cluster was horizontally acquired from Weissella cibaria, enhancing the biofilm formation ability of the host strain and its tolerance to harsh environmental stresses, including heat, acid, and bile. Transfer of cpsWc also conferred the probiotic properties to other L. plantarum strains. Moreover, cpsWc strengthened the adhesion of LTC-113 to intestinal epithelial cells. Both the cpsWc-carrying LTC-113 and its EPSs per se effectively attenuated the LPS-induced pro-inflammatory effect of intestinal epithelial cells, and inhibited the adhesion of pathogenic bacteria, such as S. typhimurium and E. coli by exclusion and competition. The newly identified cpsWc gene cluster emphasized the contribution of mobile EPS-synthetic element on the probiotic activity of L. plantarum, and shed a light on the engineering of probiotic bacteria

How Well Does the ERA5 Reanalysis Capture the Extreme Climate Events Over China? Part II: Extreme Temperature

The fifth-generation atmospheric reanalysis of the European Center for Medium-Range Weather Forecasts (ERA5) is the latest reanalysis product. However, the reliability of ERA5 to capture extreme temperatures is still unclear over China. Hence, based on conventional meteorological station data, a new criterion (DISO) was used to validate the ERA5 capturing extreme temperature indices derived from the Expert Team on Climate Change Detection and Indices (ETCCDI) across the six subregions of China on different timescales. The conclusions are as follows: the original daily temperatures (mean temperature, maximum temperature, and minimum temperature) can be well reproduced by ERA5 reanalysis over China. ERA5 tends to exhibit more misdetection for the duration of extreme temperature events than extreme temperature intensity and frequency. In addition, ERA5 performed best in the summer and worst in the winter, respectively. The trend of absolute indices (e.g., TXx and TNx), percentile-based indices (e.g., TX90p, TX10p, TN90p, and TN10p), and duration indices (e.g., WSDI, CSDI, and GSL) can be captured by ERA5, but ERA5 failed to capture the tendency of the diurnal temperature range (DTR) over China. Spatially, ERA5 performs well in southeastern China. However, it remains challenging to accurately recreate the extreme temperature events in the Tibetan Plateau. The elevation difference between the station and ERA5 grid point contributes to the main bias of reanalysis temperatures. The accuracy of ERA5 decreases with the increase in elevation discrepancy

The LiaFSR and BsrXRS Systems Contribute to Bile Salt Resistance in Enterococcus faecium Isolates

Two-component systems (TCSs) are dominant regulating components in bacteria for responding to environmental stimuli. However, little information is available on how TCSs in Enterococcus faecium respond to bile salts – an important environmental stimulus for intestinal bacteria. In this study, the gene expression of 2 TCSs, BsrXRS and LiaFSR, was positively correlated with survival rates of different E. faecium isolates during exposure to ox gall. Moreover, gene disruptions of bsrR, bsrS, liaS, and liaR significantly reduced the survival rates of E. faecium in the presence of ox gall. Finally, EMSA results indicated that BsrR functioned as a transcription regulator for expression of its own gene as well as lipoate-protein ligase A (lplA). Additional 27 potential target genes by BsrR were revealed through in silico analyses. These findings suggest that BsrXRS and LiaFSR systems play important roles in bile salt resistance in E. faecium