Nutrient availability contributes to structural and functional diversity of microbiome in Xinjiang oilfield

Indigenous microbial enhanced oil recovery (IMEOR) is a promising alternative way to promote oil recovery. It activates oil recovery microorganisms in the reservoir by adding nutrients to the injected water, utilizing microbial growth and metabolism to enhance recovery. However, few studies have focused on the impact of injected nutrients on reservoir microbial community composition and potential functions. This limits the further strategic development of IMEOR. In this study, we investigated the effects of nutrition on the composition of the reservoir bacterial community and functions in the Qizhong block of Xinjiang Oilfield, China, by constructing a long core microbial flooding simulation device. The results showed that the microbial community structure of the reservoir changed from aerobic state to anaerobic state after nutrient injection. Reducing the nutrient concentration increased the diversity and network stability of the reservoir bacterial community. At the same time, the nitrogen metabolism function also showed the same change response. Overall, these results indicated that nutrition significantly affected the community structure and function of reservoir microorganisms. Injecting low concentrations of nutrients may be more beneficial to improve oil recovery. This study is of great significance for guiding IMEOR technology and saving costs at the field site

Common genetic variants of the ion channel transient receptor potential membrane melastatin 6 and 7 (TRPM6 and TRPM7), magnesium intake, and risk of type 2 diabetes in women

<p>Abstract</p> <p>Background</p> <p>Ion channel transient receptor potential membrane melastatin 6 and 7 (TRPM6 and TRPM7) play a central role in magnesium homeostasis, which is critical for maintaining glucose and insulin metabolism. However, it is unclear whether common genetic variation in <it>TRPM6 </it>and <it>TRPM7 </it>contributes to risk of type 2 diabetes.</p> <p>Methods</p> <p>We conducted a nested case-control study in the Women's Health Study. During a median of 10 years of follow-up, 359 incident diabetes cases were diagnosed and matched by age and ethnicity with 359 controls. We analyzed 20 haplotype-tagging single nucleotide polymorphisms (SNPs) in <it>TRPM6 </it>and 5 common SNPs in <it>TRPM7 </it>for their association with diabetes risk.</p> <p>Results</p> <p>Overall, there was no robust and significant association between any single SNP and diabetes risk. Neither was there any evidence of association between common <it>TRPM6 </it>and <it>TRPM7 </it>haplotypes and diabetes risk. Our haplotype analyses suggested a significant risk of type 2 diabetes among carriers of both the rare alleles from two non-synomous SNPs in <it>TRPM6 </it>(Val1393Ile in exon 26 [rs3750425] and Lys1584Glu in exon 27 [rs2274924]) when their magnesium intake was lower than 250 mg per day. Compared with non-carriers, women who were carriers of the haplotype 1393Ile-1584Glu had an increased risk of type 2 diabetes (OR, 4.92, 95% CI, 1.05–23.0) only when they had low magnesium intake (<250 mg/day).</p> <p>Conclusion</p> <p>Our results provide suggestive evidence that two common non-synonymous <it>TRPM6 </it>coding region variants, Ile1393Val and Lys1584Glu polymorphisms, might confer susceptibility to type 2 diabetes in women with low magnesium intake. Further replication in large-scale studies is warranted.</p

Investigation and Application of a New Low-Carbon Material (Preplaced Aggregate Concrete) in Concrete-Filled Steel Tube Stub Columns

As a new low-carbon material, development of preplaced aggregate concrete (PAC) will achieve huge economic and social benefits. However, few existing research is focused on applying PAC in structural elements. This paper is attempt to apply PAC in concrete-filled steel tube (CFST) stub columns and the bearing behaviors of PAC-filled steel tube (PACFST) stub columns under axial compression are also experimentally investigated. The results indicate that the failure modes of PACFST stub columns are all drum-like failure mode which are analogous to that of CFST stub columns. The axial load-axial strain curves of PACFST stub columns can be roughly divided into elastic stage, elastic-plastic stage and plastic stage. Under the similar ultimate load, the ultimate strains are a bit smaller than that of CFST stub columns. Comparison of the results of ultimate load of PACFST stub columns calculated using the existing relevant standards for the bearing capacity calculation methods of CFST stub columns, GB 50936 and JGJ 138 are much more suitable to assess the bearing capacity of PACFST stub columns. Approximately 15%~20% saving in cement consumption will be accomplished with popularization and utilization of PACFST stub columns as compared with CFST stub columns

Development and Investigation of a New Low-Cement-Consumption Concrete—Preplaced Aggregate Concrete

Reducing consumption of cement in concrete will achieve huge benefits in decline of carbon emission, conservation of natural resources and reduction of the cost of concrete. In this paper, the low-cement-consumption concrete, preplaced aggregate concrete (PAC), is prepared and 12 types of mixtures including four water&ndash;binder ratios (W/B) and three sand&ndash;binder ratios (S/B) are designed to detect the effect of W/B and S/B on the mechanical properties and failure mechanism of PAC. Experimental and analytic results indicate that the cubic compressive strength of PAC, splitting tensile strength of PAC and elastic modulus of PAC decrease with increase in W/B and S/B. At a similar compressive strength, more than 20% increment of elastic modulus of PAC is achieved when compared with normal concrete (NC); the descent stage of stress&ndash;strain curves of PAC are steeper than that of NC and the peak strains of PAC is lower than that of NC. Guo&rsquo;s model with suitable values of parameters a and b can be used to describe the stress&ndash;strain relationship of PAC. Replacing NC by PAC in concrete structures will save 15&ndash;20% cement and achieve great environmental and economic benefits

Fresh and Mechanical Properties of Self-Compacting Rubber Lightweight Aggregate Concrete and Corresponding Mortar

Increasing amount of waste tires caused huge environment issues in recent years. Recycling concrete is an effective way. In this paper, waste tires are crushed into particles and incorporated in lightweight aggregate concrete to prepare a special concrete (self-compacting rubber lightweight aggregate concrete (SCRLC)). A detailed experimental research of effects of rubber particles on the properties of SCRLC and corresponding mortar is conducted. The results show that increasing the rubber particles replacement ratio leads to a raising of yield stress and plastic viscosity of mortar pastes. Flowability, filling capacity, and passing ability of SCRLC decline and the segregation resistance property of SCRLC improves as the rubber particles replacement ratio increases. Well, linear correlations between slump flow of SCRLC and shear stress of corresponding mortar pastes and segregation ratio of SCRLC and plastic viscosity of corresponding mortar pastes are obtained. In order to ensure that rubber lightweight aggregate concrete can compact by itself, the upper limit of shear stress of corresponding mortar pastes is 231.7 Pa and the lower limit of plastic viscosity of corresponding mortar pastes is 3.72 Pa·s. Compressive strength, splitting tensile strength, flexural strength, and elastic modulus of SCRLC and compressive strength of corresponding mortar decrease as the rubber particles replacement ratio increases. The 28-day compressive strength of SCRLC can meet the requirements of lightweight aggregate concrete structures until the rubber particles replacement ratio reaches 50%

Research on the Bond Behavior of Preplaced Aggregate Concrete-Filled Steel Tube Columns

In order to investigate the bond behavior of preplaced aggregate concrete-filled steel tube (CFT-PAC) columns and the difference of bond behavior between CFT-PAC columns and normal concrete-filled steel tube (CFT-NC) columns, a total of 11 columns were prepared and the push-out tests were conducted. The experimental parameters included the type of concrete (preplaced aggregate concrete and normal concrete), concrete strength (C40, C50 and C60), cross-section dimension (D = 219 mm, 299 mm and 351 mm) and the thickness of steel tube (t = 6 mm and 8 mm). The results indicated that the CTF-PAC columns had a similar load-slip curves with CFT-NC columns. The bond stresses of the CFT-PAC columns were higher than that of the PAC-NC columns at the same concrete strength. Increasing compressive strength of PAC increased the critical bond strength and bond strength of CFT-PAC columns. With an increase of the L/D ratio, both of the slip corresponding to peak load and bond strength of CFT-PAC columns exhibited an increasing trend. A rise in the D/t ratio led to a decrease in the bond stress of CFT-PAC columns and an increase in slip corresponding to the peak load of CFT-PAC columns. The proposed bond stress&ndash;slip relationship model considerably matched the bond stress&ndash;slip relationship of CFT-PAC columns

A New Composite Slab Using Crushed Waste Tires as Fine Aggregate in Self-Compacting Lightweight Aggregate Concrete

A composite slab comprised of self-compacting rubber lightweight aggregate concrete (SCRLC) and profiled steel sheeting is a new type of structural element with a series of superior properties. This paper presents an experimental research and finite element analysis (FEA) of the flexural behavior of composite slabs consisting of SCRLC to develop a new floor system. Four composite slabs specimens with different shear spans (450 mm and 800 mm) and SCRLC (0% and 30% in rubber particles substitution ratio) are prepared, and the flexural properties including failure modes, deflection at mid-span, profiled steel sheeting, and concrete surface stain at mid-span and end slippage are investigated by four-point bending tests. The experimental results indicate that applying SCRLC30 in composites slabs will improve the anti-cracking ability under the loading of composite slabs compared with composite slabs consisting of self-compacting lightweight aggregate concrete (SCLC). FEM on the flexural properties of SCRLC composites slabs show that the yield load, ultimate load, and deflection corresponding to the yield load and the ultimate load of composite slabs drop as the rubber particles content increases in SCRLC. The variation of SCRLC strength has less impact on the flexural bearing capacity of corresponding composite slabs. Based on the traditional calculated method of the ultimate bending moment of normal concrete (NC) composite slabs, a modified calculated method for the ultimate bending moment of SCRLC composite slabs is proposed