Necrotic potential of BiV on murine macrophages <i>in vitro</i>.

<p>[A-C] Dot plot diagrams representing respectively control and groups treated with BiV at 50 μg/mL and 100 μg/mL. [D] Increase in the percentage of PI-labeled cells, suggestive of cell death by necrosis. *p<0.05 vs. control group.</p

Analyses of nitrate/nitrite concentrations in macrophages after treatment with BiV with and without L-NAME.

<p>The analysis was performed using Griess method with modifications. *p<0.05 vs. control group.</p

Time- and concentration-dependent biological effect caused by BiV on RAW 264.7 cells.

<p>Cell viability was determined after [A] 2 hours, [B] 6 hours, [C] 12 hours and [D] 24 hours of incubation with BiV, using MTT reduction assay. *p<0.05 vs. control group.</p

Cell number in machophages treated with BiV.

<p>The cell density was determined by counting in Neubauer chamber. *p<0.05 vs. negative control group.</p

Enzymatic activities exhibited by BiV.

<p>[A] Proteolytic activity, assessed by azocasein cleavage method; [B] phospholipasic assay, determined using 4N3OBA as the lipidic substrate; and [C] H₂O₂ production induced by BiV, suggestive of the presence of LAAO. *p<0.05 vs control group (absence of venom).</p

Elongation on aliphatic chain improves selectivity of 2-hydroxy-3,4,6-trimethoxyphenyl chalcone on Trypanosoma cruzi

Suppl data</p