306 research outputs found
13 Years of Timing of PSR B1259-63
This paper summarizes the results of 13 years of timing observations of a
unique binary pulsar, PSR B125963, which has a massive B2e star companion.
The data span encompasses four complete orbits and includes the periastron
passages in 1990, 1994, 1997 and 2000. Changes in dispersion measure occurring
around the 1994, 1997 and 2000 periastrons are measured and accounted for in
the timing analysis. There is good evidence for a small glitch in the pulsar
period in 1997 August, not long after the 1997 periastron, and a significant
frequency second derivative indicating timing noise. We find that spin-orbit
coupling with secular changes in periastron longitude and projected semi-major
axis () cannot account for the observed period variations over the whole
data set. While fitting the data fairly well, changes in pulsar period
parameters at each periastron seem ruled out both by X-ray observations and by
the large apparent changes in pulsar frequency derivative. Essentially all of
the systematic period variations are accounted for by a model consisting of the
1997 August glitch and step changes in at each periastron. These changes
must be due to changes in the orbit inclination, but we can find no plausible
mechanism to account for them. It is possible that timing noise may mask the
actual changes in orbital parameters at each periastron, but the good fit to
the data of the step-change model suggests that short-term timing noise is
not significant.Comment: 9 pages, 7 figures, accepted by MNRA
Functional and Topological Properties in Hepatocellular Carcinoma Transcriptome
Hepatocellular carcinoma (HCC) is a leading cause of global cancer mortality. However, little is known about the precise molecular mechanisms involved in tumor formation and pathogenesis. The primary goal of this study was to elucidate genome-wide molecular networks involved in development of HCC with multiple etiologies by exploring high quality microarray data. We undertook a comparative network analysis across 264 human microarray profiles monitoring transcript changes in healthy liver, liver cirrhosis, and HCC with viral and alcoholic etiologies. Gene co-expression profiling was used to derive a consensus gene relevance network of HCC progression that consisted of 798 genes and 2,012 links. The HCC interactome was further confirmed to be phenotype-specific and non-random. Additionally, we confirmed that co-expressed genes are more likely to share biological function, but not sub-cellular localization. Analysis of individual HCC genes revealed that they are topologically central in a human protein-protein interaction network. We used quantitative RT-PCR in a cohort of normal liver tissue (n = 8), hepatitis C virus (HCV)-induced chronic liver disease (n = 9), and HCC (n = 7) to validate co-expressions of several well-connected genes, namely ASPM, CDKN3, NEK2, RACGAP1, and TOP2A. We show that HCC is a heterogeneous disorder, underpinned by complex cross talk between immune response, cell cycle, and mRNA translation pathways. Our work provides a systems-wide resource for deeper understanding of molecular mechanisms in HCC progression and may be used further to define novel targets for efficient treatment or diagnosis of this disease
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Understanding aerosol microphysical properties from 10 years of data collected at Cabo Verde based on an unsupervised machine learning classification
The Cape Verde Atmospheric Observatory (CVAO), which is influenced by both marine and desert dust air masses, has been used for long-term measurements of different properties of the atmospheric aerosol from 2008 to 2017. These properties include particle number size distributions (PNSD), light-absorbing carbon (LAC) and concentrations of cloud condensation nuclei (CCN) together with their hygroscopicity. Here we summarize the results obtained for these properties and use an unsupervised machine learning algorithm for the classification of aerosol types. Five types of aerosols, i.e., marine, freshly formed, mixture, moderate dust and heavy dust, were classified. Air masses during marine periods are from the Atlantic Ocean and during dust periods are from the Sahara Desert. Heavy dust was more frequently present during wintertime, whereas the clean marine periods were more frequently present during springtime. It was observed that during the dust periods CCN number concentrations at a supersaturation of 0.30g% were roughly 2.5 times higher than during marine periods, but the hygroscopicity (κ) of particles in the size range from g1/4g30 to g1/4g175gnm during marine and dust periods were comparable. The long-term data presented here, together with the aerosol classification, can be used as a basis to improve our understanding of annual cycles of the atmospheric aerosol in the eastern tropical Atlantic Ocean and on aerosol-cloud interactions and it can be used as a basis for driving, evaluating and constraining atmospheric model simulations
Surgical resection and survival of patients with unsuspected single node positive lung cancer (NSCLC) invading the descending aorta
Background: Surgical treatment of non-small cell lung cancer (NSCLC) with aortic invasion is still debated
Mucosal Progranulin expression is induced by H. pylori, but independent of Secretory Leukocyte Protease Inhibitor (SLPI) expression
<p>Abstract</p> <p>Background</p> <p>Mucosal levels of Secretory Leukocyte Protease Inhibitor (SLPI) are specifically reduced in relation to <it>H. pylori</it>-induced gastritis. Progranulin is an epithelial growth factor that is proteolytically degraded into fragments by elastase (the main target of SLPI). Considering the role of SLPI for regulating the activity of elastase, we studied whether the <it>H. pylori</it>-induced reduction of SLPI and the resulting increase of elastase-derived activity would reduce the Progranulin protein levels both <it>ex vivo </it>and <it>in vitro</it>.</p> <p>Methods</p> <p>The expression of Progranulin was studied in biopsies of <it>H. pylori</it>-positive, -negative and -eradicated subjects as well as in the gastric tumor cell line AGS by ELISA, immunohistochemistry and real-time RT-PCR.</p> <p>Results</p> <p><it>H. pylori</it>-infected subjects had about 2-fold increased antral Progranulin expression compared to <it>H. pylori</it>-negative and -eradicated subjects (P < 0.05). Overall, no correlations between mucosal Progranulin and SLPI levels were identified. Immunohistochemical analysis confirmed the upregulation of Progranulin in relation to <it>H. pylori </it>infection; both epithelial and infiltrating immune cells contributed to the higher Progranulin expression levels. The <it>H. pylori</it>-induced upregulation of Progranulin was verified in AGS cells infected by <it>H. pylori</it>. The down-regulation of endogenous SLPI expression in AGS cells by siRNA methodology did not affect the Progranulin expression independent of the infection by <it>H. pylori</it>.</p> <p>Conclusions</p> <p>Taken together, Progranulin was identified as novel molecule that is upregulated in context to <it>H. pylori </it>infection. In contrast to other diseases, SLPI seems not to have a regulatory role for Progranulin in <it>H. pylori</it>-mediated gastritis.</p
Experimental study of the role of physicochemical surface processing on the IN ability of mineral dust particles
During the measurement campaign FROST 2 (FReezing Of duST 2), the Leipzig Aerosol Cloud Interaction Simulator (LACIS) was used to investigate the influence of various surface modifications on the ice nucleating ability of Arizona Test Dust (ATD) particles in the immersion freezing mode. The dust particles were exposed to sulfuric acid vapor, to water vapor with and without the addition of ammonia gas, and heat using a thermodenuder operating at 250 °C. Size selected, quasi monodisperse particles with a mobility diameter of 300 nm were fed into LACIS and droplets grew on these particles such that each droplet contained a single particle. Temperature dependent frozen fractions of these droplets were determined in a temperature range between −40 °C ≤T≤−28 °C. The pure ATD particles nucleated ice over a broad temperature range with their freezing behavior being separated into two freezing branches characterized through different slopes in the frozen fraction vs. temperature curves. Coating the ATD particles with sulfuric acid resulted in the particles' IN potential significantly decreasing in the first freezing branch (T>−35 °C) and a slight increase in the second branch (T≤−35 °C). The addition of water vapor after the sulfuric acid coating caused the disappearance of the first freezing branch and a strong reduction of the IN ability in the second freezing branch. The presence of ammonia gas during water vapor exposure had a negligible effect on the particles' IN ability compared to the effect of water vapor. Heating in the thermodenuder led to a decreased IN ability of the sulfuric acid coated particles for both branches but the additional heat did not or only slightly change the IN ability of the pure ATD and the water vapor exposed sulfuric acid coated particles. In other words, the combination of both sulfuric acid and water vapor being present is a main cause for the ice active surface features of the ATD particles being destroyed. A possible explanation could be the chemical transformation of ice active metal silicates to metal sulfates. The strongly enhanced reaction between sulfuric acid and dust in the presence of water vapor and the resulting significant reductions in IN potential are of importance for atmospheric ice cloud formation. Our findings suggest that the IN concentration can decrease by up to one order of magnitude for the conditions investigated
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Arctic haze over Central Europe
An extraordinary aerosol situation over Leipzig, Germany in April 2002 was investigated with a comprehensive
set of ground-based volumetric and columnar aerosol data, combined with aerosol profiles
from lidar, meteorological data from radiosondes and air mass trajectory calculations. Air masses
were identified to stem from the Arctic, partly influenced by the greater Moscow region. An evaluation
of ground-based measurements of aerosol size distributions during these periods showed that
the number concentrations below about 70 nm in diameter were below respective long-term average
data, while number, surface and volume concentrations of the particles larger than about 70 nm in
diameter were higher than the long-term averages. The lidar aerosol profiles showed that the imported
aerosol particles were present up to about 3 km altitude. The particle optical depth was up to 0.45 at
550 nm wavelength. With a one-dimensional spectral radiative transfer model top of the atmosphere
(TOA) radiative forcing of the aerosol layer was estimated for a period with detailed vertical information.
Solar aerosol radiative forcing values between −23 and −38 W m−2 were calculated, which are
comparable to values that have been reported in heavily polluted continental plumes outside the respective
source regions. The present report adds weight to previous findings of aerosol import to Europe,
pointing to the need for attributing the three-dimensional aerosol burden to natural and anthropogenic
sources as well as to aerosol imports from adjacent or distant source regions. In the present case, the
transport situation is further complicated by forward trajectories, indicating that some of the observed
Arctic haze may have originated in Central Europe. This aerosolwas transported to the European Arctic
before being re-imported in the modified and augmented form to its initial source region
Characterization of aerosol properties at Cyprus, focusing on cloud condensation nuclei and ice-nucleating particles
As part of the A-LIFE (Absorbing aerosol layers in a changing climate: aging, LIFEtime and dynamics) campaign, ground-based measurements were carried out in Paphos, Cyprus, to characterize the abundance, properties, and sources of aerosol particles in general and cloud condensation nuclei (CCN) and ice-nucleating particles (INP) in particular. New particle formation (NPF) events with subsequent growth of the particles into the CCN size range were observed. Aitken mode particles featured κ values of 0.21 to 0.29, indicating the presence of organic materials. Accumulation mode particles featured a higher hygroscopicity parameter, with a median κ value of 0.57, suggesting the presence of sulfate and maybe sea salt particles mixed with organic carbon. A clear downward trend of κ with increasing supersaturation and decreasing dcrit was found. Super-micron particles originated mainly from sea-spray aerosol (SSA) and partly from mineral dust. INP concentrations (NINP) were measured in the temperature range from -6.5 to -26.5 °C, using two freezing array-type instruments. NINP at a particular temperature span around 1 order of magnitude below -20 °C and about 2 orders of magnitude at warmer temperatures (T > -18 °C). Few samples showed elevated concentrations at temperatures > -15 °C, which suggests a significant contribution of biological particles to the INP population, which possibly could originate from Cyprus. Both measured temperature spectra and NINP probability density functions (PDFs) indicate that the observed INP (ice active in the temperature range between -15 and -20 °C) mainly originate from long-range transport. There was no correlation between NINP and particle number concentration in the size range> 500 nm (N>500 nm). Parameterizations based on N>500 nm were found to overestimate NINP by about 1 to 2 orders of magnitude. There was also no correlation between NINP and particle surface area concentration. The ice active surface site density (ns) for the polluted aerosol encountered in the eastern Mediterranean in this study is about 1 to 3 orders of magnitude lower than the ns found for dust aerosol particles in previous studies. This suggests that observed NINP PDFs such as those derived here could be a better choice for modeling NINP if the aerosol particle composition is unknown or uncertain
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