Structural and functional studies of STAT1 from Atlantic salmon (Salmo salar)

<p>Abstract</p> <p>Background</p> <p>Type I and type II interferons (IFNs) exert their effects mainly through the JAK/STAT pathway, which is presently best described in mammals. STAT1 is involved in signaling pathways induced by both types of IFNs. It has a domain-like structure including an amino-terminus that stabilizes interaction between STAT dimers in a promoter-binding situation, a coiled coil domain facilitating interactions to other proteins, a central DNA-binding domain, a SH2 domain responsible for dimerization of phosphorylated STATs and conserved phosphorylation sites within the carboxy terminus. The latter is also the transcriptional activation domain.</p> <p>Results</p> <p>A salmon (<it>Salmo salar</it>) STAT1 homologue, named ssSTAT1a, has been identified and was shown to be ubiquitously expressed in various cells and tissues. The ssSTAT1a had a domain-like structure with functional motifs that are similar to higher vertebrates. Endogenous STAT1 was shown to be phosphorylated at tyrosine residues both in salmon leukocytes and in TO cells treated with recombinant type I and type II IFNs. Also ectopically expressed ssSTAT1 was phosphorylated in salmon cells upon <it>in vitro </it>stimulation by the IFNs, confirming that the cloned gene was recognized by upstream tyrosine kinases. Treatment with IFNs led to nuclear translocation of STAT1 within one hour. The ability of salmon STAT1 to dimerize was also shown.</p> <p>Conclusions</p> <p>The structural and functional properties of salmon STAT1 resemble the properties of mammalian STAT1.</p

Further empirical evidence for the non-linearity of the period-luminosity relations as seen in the Large Magellanic Cloud Cepheids

(abridged) Recent studies, using OGLE data for LMC Cepheids in the optical, strongly suggest that the period-luminosity (PL) relation for the Large Magellanic Cloud (LMC) Cepheids shows a break or non-linearity at a period of 10 days. In this paper we apply statistical tests, the chi-square test and the F-test, to the Cepheid data from the MACHO project to test for a non-linearity of the V- and R-band PL relations at 10 days, and extend these tests to the near infrared (JHK-band) PL relations with 2MASS data. We correct the extinction for these data by applying an extinction map towards the LMC. The statistical test we use, the F-test, is able to take account of small numbers of data points and the nature of that data on either side of the period cut at 10 days. With our data, the results we obtained imply that the VRJH-band PL relations are non-linear around a period of 10 days, while the K-band PL relation is (marginally) consistent with a single-line regression. The choice of a period of 10 days, around which this non-linearity occurs, is consistent with the results obtained when this "break" period is estimated from the data. Long period Cepheids are supplemented from the literature to increase our sample size. The photometry of these long period Cepheids is compared with our data and no trend with period is found. Our main results remain unchanged when we supplement our dataset with these long period Cepheids. By examining our data at maximum light, we also suggest arguments why errors in reddening are unlikely to be responsible for our results. The non-linearity of the mean V-band PL relation as seen in both of the OGLE and MACHO data, using different extinction maps, suggests that this non-linearity is real.Comment: 18 pages, 10 tables, 7 figures. MNRAS accepte

On-chip interrogator based on Fourier Transform spectroscopy

In this paper, the design and the characterization of a novel interrogator based on integrated Fourier transform (FT) spectroscopy is presented. To the best of our knowledge, this is the first integrated FT spectrometer used for the interrogation of photonic sensors. It consists of a planar spatial heterodyne spectrometer, which is implemented using an array of Mach-Zehnder interferometers (MZIs) with different optical path differences. Each MZI employs a 3$\times$3 multi-mode interferometer, allowing the retrieval of the complex Fourier coefficients. We derive a system of non-linear equations whose solution, which is obtained numerically from Newton's method, gives the modulation of the sensor's resonances as a function of time. By taking one of the sensors as a reference, to which no external excitation is applied and its temperature is kept constant, about 92$\%$ of the thermal induced phase drift of the integrated MZIs has been compensated. The minimum modulation amplitude that is obtained experimentally is 400 fm, which is more than two orders of magnitude smaller than the FT spectrometer resolution.Comment: 15 pages, 6 figure

Dynamical mass of a star cluster in M83: a test of fibre-fed multi-object spectroscopy

(Abridged) Aims: We obtained VLT/FLAMES+UVES high-resolution, fibre-fed spectroscopy (FFS) of five young massive clusters in M83 (NGC 5236). This forms the basis of a pilot study testing the feasibility of using FFS to measure the velocity dispersions of several clusters simultaneously, in order to determine their dynamical masses; Methods: We adopted two methods for determining the velocity dispersion of the star clusters: cross-correlating the cluster spectrum with the template spectra and minimising a chi^2 value between the cluster spectrum and the broadened template spectra. Cluster 805 in M83 was chosen as a control to test the reliability of the method, through a comparison with the results obtained from a standard echelle VLT/UVES spectrum obtained by Larsen & Richtler; Results: We find no dependence of the velocity dispersions measured for a cluster on the choice of red giant versus red supergiant templates, nor on the method adopted. We measure a velocity dispersion of sigma_los = 10.2+/-1.1 km/s for cluster 805 from our FFS. Our FLAMES+UVES velocity dispersion measurement gives M_vir = (6.6+/-1.7)e5 M_sun, consistent with previous results. This is a factor of ~3 greater than the cluster's photometric mass, indicating a lack of virial equilibrium. However, based on its effective star formation efficiency, the cluster is likely to virialise, and may survive for a Hubble time, in the absence of external disruptive forces; Conclusions: We find that reliable velocity dispersions can be determined from FFS. The advantages of observing several clusters simultaneously outweighs the difficulty of accurate galaxy background subtraction, providing that the targets are chosen to provide sufficient S/N ratios, and are much brighter than the galaxy background.Comment: 10 pages, 4 figures, accepted by A&

A Study of Cepheids in M81 with the Large Binocular Telescope (Efficiently Calibrated with HST)

We identify and phase a sample of 107 Cepheids with 10<P/days<100 in M81 using the LBT and calibrate their BVI mean magnitudes with archival HST data. The use of a ground-based telescope to identify and phase the Cepheids and HST only for the final calibration reduces the demand on HST by nearly an order of magnitude and yields Period-Luminosity (PL) relations with dispersions comparable to the best LMC samples. We fit the sample using the OGLE-II LMC PL relations and are unable to find a self-consistent distance for different band combinations or radial locations within M81. We can do so after adding a radial dependence to the PL zero point that corresponds to a luminosity dependence on metallicity of g_mu=-0.56+/-0.36 mag/dex. We find marginal evidence for a shift in color as a function of metallicity, distinguishable from the effects of extinction, of g_2=+0.07+/-0.03 mag/dex. We find a distance modulus for M81, relative to the LMC, of mu(M81-LMC)=9.39+/-0.14 mag, including uncertainties due to the metallicity corrections. This corresponds to a distance to M81 of 3.6+/-0.2 Mpc, assuming a LMC distance modulus of 18.41 mag. We carry out a joint analysis of M81 and NGC4258 Cepheids and simultaneously solve for the distance of M81 relative to NGC4258 and the metallicity corrections. Given the current data, the uncertainties of such joint fits are dominated by the relative metallicities and the abundance gradients rather than by measurement errors of the Cepheid magnitudes or colors. We find mu(M81-LMC)=9.40 (-0.11/+0.15) mag, mu(N4258-LMC)=11.08 (-0.17/+0.21) mag and mu(N4258-M81)=1.68+/-0.08 mag and joint metallicity corrections of g_mu=-0.62 (-0.35/+0.31) mag/dex and g_2=0.01+/-0.01 mag/dex. Quantitative analyses of Cepheid distances must take into account both the metallicity dependencies of the Cepheids and the uncertainties in the abundance estimates. (ABRIDGED)Comment: 45 pages, 14 figures, 4 tables, appeared in The Astrophysical Journa

Cepheid Calibration of the Peak Brightness of SNe Ia. X. SN 1991T in NGC 4527

Repeated imaging observations have been made of NGC 4527 with the Hubble Space Telescope between April and June 1999, over an interval of 69 days. Images were obtained on 12 epochs in the F555W band and on five epochs in the F814W band. The galaxy hosted the type Ia supernova SN1991T, which showed relatively unusual behavior by having both an abnormal spectrum near light maximum, and a slower declining light curve than the proto-typical Branch normal SNe Ia. A total of 86 variables that are putative Cepheids have been found, with periods ranging from 7.4 days to over 70 days. From photometry with the DoPHOT program, the de-reddened distance modulus is determined to be (m-M)_0 = 30.67 +/- 0.12 (internal uncertainty) using a subset of the Cepheid data whose reddening and error parameters are secure. A parallel analysis of the Cepheids using photometry with ROMAFOT yields (m -M)_0 =30.82 +/- 0.11. The final adopted modulus is (m -M)_0 =30.74 +/- 0.12 +/- 0.12 (d=14.1 +/- 0.8 +/- 0.8 Mpc). The photometric data for SN1991T are used in combination with the Cepheid distance to NGC 4527 to obtain the absolute magnitude for this supernova of M_V^0(max) = -19.85 +/- 0.29. The relatively large uncertainty is a result of the range in estimates of the reddening to the supernova. Thus SN1991T is seen to be only moderately brighter (by ~ 0.3 mag) than the mean for spectroscopically normal supernovae, although magnitude differences of up to 0.6 mag cannot be ruled out.Comment: 46 pages, LATEX using aaspp4.sty, including 9 embedded tables, 19 figures (gif and jpg files), a full-resolution version (ps files) is available at http://www.astro.unibas.ch/forschung/ll/cepheid.shtml, accepted for publication in the Astrophysical Journa

Cepheids and Long Period Variables in NGC 4395

Repeated imaging observations of NGC 4395 were made with the WIYN 3.5 m and the KPNO 2.1 m telescopes. From the photometry of the resolved brighter stars in this galaxy eleven Cepheids with periods ranging between 12 and 90 days have been identified. The true distance modulus has been derived from the apparent distance moduli in g, r and i. The distance modulus is 28.02 +/- 0.18 based on the LMC P-L relation by Sandage et al. 2003; this corresponds to a distance of 4.0 +/- 0.3 Mpc. Using the P-L relation from Madore & Freedman 1991, the distance modulus is 28.15 +/- 0.18; which corresponds to a distance of 4.3 +/- 0.4 Mpc. The reddening is calculated to be E(g-r) = 0.06 +/- 0.08 and E(r-i) = 0.10 +/- 0.08, again from the distance moduli mu_g, mu_r and mu_i. In addition, 37 other variables have been detected, the majority of which have definite periods. They are probably all red long period variables.Comment: 54 pages, 8 figures, 8 tables, accepted for publication in the Astronomical Journa