3 research outputs found
Angiogenesis in cancer of unknown primary: clinicopathological study of CD34, VEGF and TSP-1-0
<p><b>Copyright information:</b></p><p>Taken from "Angiogenesis in cancer of unknown primary: clinicopathological study of CD34, VEGF and TSP-1"</p><p>BMC Cancer 2005;5():25-25.</p><p>Published online 3 Mar 2005</p><p>PMCID:PMC555600.</p><p>Copyright © 2005 Karavasilis et al; licensee BioMed Central Ltd.</p
Proteome Changes during Transition from Human Embryonic to Vascular Progenitor Cells
Human
embryonic stem cells (hESCs) are promising in regenerative
medicine (RM) due to their differentiation plasticity and proliferation
potential. However, a major challenge in RM is the generation of a
vascular system to support nutrient flow to newly synthesized tissues.
Here we refined an existing method to generate tight vessels by differentiating
hESCs in CD34<sup>+</sup> vascular progenitor cells using chemically
defined media and growth conditions. We selectively purified these
cells from CD34<sup>–</sup> outgrowth populations also formed.
To analyze these differentiation processes, we compared the proteomes
of the hESCs with those of the CD34<sup>+</sup> and CD34<sup>–</sup> populations using high resolution mass spectrometry, label-free
quantification, and multivariate analysis. Eighteen protein markers
validate the differentiated phenotypes in immunological assays; nine
of these were also detected by proteomics and show statistically significant
differential abundance. Another 225 proteins show differential abundance
between the three cell types. Sixty-three of these have known functions
in CD34<sup>+</sup> and CD34<sup>–</sup> cells. CD34<sup>+</sup> cells synthesize proteins implicated in endothelial cell differentiation
and smooth muscle formation, which support the bipotent phenotype
of these progenitor cells. CD34<sup>–</sup> cells are more
heterogeneous synthesizing muscular/osteogenic/chondrogenic/adipogenic
lineage markers. The remaining >150 differentially abundant proteins
in CD34<sup>+</sup> or CD34<sup>–</sup> cells raise testable
hypotheses for future studies to probe vascular morphogenesis
Proteome Changes during Transition from Human Embryonic to Vascular Progenitor Cells
Human
embryonic stem cells (hESCs) are promising in regenerative
medicine (RM) due to their differentiation plasticity and proliferation
potential. However, a major challenge in RM is the generation of a
vascular system to support nutrient flow to newly synthesized tissues.
Here we refined an existing method to generate tight vessels by differentiating
hESCs in CD34<sup>+</sup> vascular progenitor cells using chemically
defined media and growth conditions. We selectively purified these
cells from CD34<sup>–</sup> outgrowth populations also formed.
To analyze these differentiation processes, we compared the proteomes
of the hESCs with those of the CD34<sup>+</sup> and CD34<sup>–</sup> populations using high resolution mass spectrometry, label-free
quantification, and multivariate analysis. Eighteen protein markers
validate the differentiated phenotypes in immunological assays; nine
of these were also detected by proteomics and show statistically significant
differential abundance. Another 225 proteins show differential abundance
between the three cell types. Sixty-three of these have known functions
in CD34<sup>+</sup> and CD34<sup>–</sup> cells. CD34<sup>+</sup> cells synthesize proteins implicated in endothelial cell differentiation
and smooth muscle formation, which support the bipotent phenotype
of these progenitor cells. CD34<sup>–</sup> cells are more
heterogeneous synthesizing muscular/osteogenic/chondrogenic/adipogenic
lineage markers. The remaining >150 differentially abundant proteins
in CD34<sup>+</sup> or CD34<sup>–</sup> cells raise testable
hypotheses for future studies to probe vascular morphogenesis