Plantes et virus : la guerre sans fin

National audienc

Mécanisme de résistance aux virus dans les plantes transgéniques

National audienc

Banana virus X (BVX), a novel flexivirus harbouring defective RNAs, defines a new genus within the Flexiviridae family

International audienc

Molecular characterization of Banana X virus (BanXV), a Flexivirus infecting banana

International audienc

Caractérisation moléculaire du Banana virus X (BVX), un flexivirus infectant le bananier

National audienc

Molecular variability of Banana mild mosaic virus (BanMMV)

International audienc

Transgenic tobacco plants that contain the plum pox virus (PPV) coat protein gene

International audienc

Construction of a chimeric viral gene expressing plum pox virus coat protein

International audienc

Expression of a yeast RNase III gene in transgenic tobacco silences host nitrite reductase genes

44 ref.International audienc

Molecular characterization of banana virus X (BVX), a novel member of the Flexiviridae family

International audienceA novel virus was identified in banana (Musa spp). Analysis of the last 2917 nucleotides of its positive strand genomic RNA showed five open reading frames corresponding, from 5′ to 3′, to a truncated ORF coding for a replication-associated protein, three ORFs coding for a movement-associated triple gene block (TGB) and a capsid protein (CP) gene. This genome organization is similar to that of some members of the Flexiviridae family such as potexviruses and foveaviruses. This virus was named Banana virus X (BVX). Comparative sequence analysis showed that BVX is only distantly related to other members of the Flexiviridae family, in which it appears to define a new genus. BVX produces defective RNAs derived from its genomic RNA by non-homologous recombination. Three distinct pairs of donor/acceptor recombination sites involving short direct nucleotide repeats were characterized, accounting for deletions of 1268, 1358 and 1503 nucleotides. Contrary to the situation encountered for Potexviruses, these recombination sites are located within the TGB1 and CP genes and result in a truncated TGB1 protein