Epithelioid peritoneal mesothelioma with pulmonary metastasis in feline

A feline, female, mixed breed, with two years of age and history of abdominal distension was referred to the Veterinary Hospital of the Federal University of Uberlândia for necropsy. During necropsy, multiple nodules of 0.2 to 2 cm in diameter were observed, coalescent, firm, white, with irregular surface in omentum, serosa of the stomach and large intestine, diaphragm and peritoneum. Similar millimetric nodules were seen, diffusely distributed in the lung parenchyma. The samples of the omitted nodules were submitted to histopathology and immunohistochemistry. The antibodies used were anti-vimentin, anti-cytokeratin AE1 / AE3 and anti-calretinin. Histologically, the nodules observed in the abdominal cavity and pulmonary parenchyma consisted of neoplastic proliferation of mesothelial cells in a solid arrangement. In immunohistochemical analyzes, neoplastic cells showed cytoplasmic immunostaining for cytokeratin AE1 / AE3 and calretinin, and did not exhibit immunoreactivity for vimentin. Based on the anatomopathological and immunohistochemical findings, the diagnosis of epithelioid peritoneal mesothelioma with pulmonary metastasis was attributed.Pesquisa sem auxílio de agências de fomentoUm felino, fêmea, sem raça definida, com dois anos de idade e histórico de distensão abdominal foi encaminhado ao Hospital Veterinário da Universidade Federal de Uberlândia para necropsia. Durante necropsia, observou-se múltiplos nódulos de 0,2 a 2cm de diâmetro, coalescentes, firmes, brancacentos, de superfície irregular em omento, serosa de estômago e intestino grosso, diafragma e peritônio. Foram visualizados nódulos milimétricos semelhantes distribuídos difusamente em parênquima pulmonar. As amostras dos nódulos em omento foram submetidas à histopatologia e imunoistoquímica. Os anticorpos utilizados foram anti-vimentina, anti-citoqueratina AE1/AE3 e anti-calretinina. Histologicamente, os nódulos observados cavidade abdominal e parênquima pulmonar eram constituídos por proliferação neoplásica de células mesoteliais em arranjo sólido. Nas análises imunoistoquímicas, as células neoplásicas apresentaram imunomarcação citoplasmática para citoqueratina AE1/AE3 e calretinina, e não exibiram imunorreatividade para vimentina. Com base nos achados anatomopatológicos e imunoistoquímicos atribuiu-se o diagnóstico de mesotelioma peritoneal epitelioide com metástase pulmonar.2023-03-0

ACHADOS POST MORTEM EM CÃES INTOXICADOS POR PICADAS DE ABELHAS

Bee sting poisonings have great relevance for both human and veterinary medicine. The toxin components present harmful actions on tissues, especially on kidneys, and can lead to death, even when the inoculated dose is small. The early identification of this type of intoxication allows the implementation of appropriate therapeutic measures and improvement of the prognosis. Thus, the present study aimed to report the anatomopathological findings of two mixed-breed dogs, which were fatal victims of bee stings. The animals were litter brothers, one male and one female, with three years old and weighing about 30kg. The dogs were found dead at night, already in rigor mortis, which led to the suspicion that the death had occurred no more than five hours ago. The main macroscopic lesions were: cutaneous petechiae, some of them associated with bee stingers, generalized congestion, haemorrhage, necrosis, and oedema, as well as insects with morphology compatible with Apis mellifera dispersed in the gastrointestinal tract. Microscopically, degeneration, necrosis, and renal haemorrhages were the most relevant findings, in addition to marked pulmonary oedema, which was considered the causa mortis. Thus, the most important alterations in this type of intoxication are necrosis, haemorrhage, oedema, and congestion. Moreover, death can occur quickly, even with low doses of the toxin.As intoxicações por picadas de abelhas possuem grande relevância na medicina humana e veterinária. Os componentes da toxina apresentam ações lesivas aos tecidos, principalmente aos rins, e podem culminar com a morte, mesmo quando a dose inoculada é pequena. A identificação precoce desse tipo de intoxicação permite a implementação de medidas terapêuticas adequadas e a melhoria do prognóstico. Desta forma, o presente estudo foi desenvolvido com o objetivo de relatar os achados anatomopatológicos observados em dois cães, sem raça definida, os quais foram vítimas fatais de picadas de abelhas. Os animais eram irmãos de ninhada, um macho e uma fêmea, com três anos de idade e com cerca de 30kg. Os cães foram encontrados mortos no período da noite, já em rigor mortis, o que conduziu à suspeita de que a morte havia ocorrido há, no máximo, cinco horas. As principais lesões macroscópicas observadas foram: petéquias cutâneas, algumas associadas à presença de ferrões; congestão generalizada; hemorragia; necrose e edema; assim como insetos com morfologia compatível com Apis mellifera dispersos no trato gastrointestinal. Microscopicamente, degeneração, necrose e hemorragias renais constituíram os achados de maior importância, além de acentuado edema pulmonar, ao qual foi atribuída a causa mortis. Assim, as alterações mais importantes neste tipo de intoxicação são necrose, hemorragia, edema e congestão. Além disso, o óbito pode ocorrer de forma rápida, mesmo com baixas doses da toxina

Metastatic Pelvic Osteosarcoma in a Dog

Background: Osteosarcomas are malignant neoplasms of bone tissue, with a high prevalence in dogs, especially in large and giant breeds. More commonly, such alterations affect the appendicular skeleton and, to a lesser extent, the axial skeleton. In order to obtain an accurate diagnosis, it is necessary to combine cytological and histopathological findings with clinical parameters, imaging exams and macroscopic findings. In the present study, we report a rare case of combined-type pelvic osteosarcoma with pulmonary metastasis in a dog.Case: A 5-year-old intact large male dog of mixed breed, was submitted to clinical care because of an increase in volume of the left perineal region.  The cytological evaluation, performed without imaging exams, indicated that it was an undifferentiated sarcoma. An incisional biopsy defined the diagnosis as telangiectatic osteosarcoma, and with progressive clinical worsening, the patient died. Necroscopic examination revealed multiple nodules in the lungs and an irregular mass with a hard to friable consistency. The mass was intensely vascularised and extended craniodorsally from the left ischial tuberosity to the base of the renal fossa. Microscopically, the neoplasm was diagnosed as combined osteosarcoma, consisting of the osteoblastic, chondroblastic, and telangiectatic subtypes. Metastases with a predominance of the chondroblastic subtype were observed in the lungs.Discussion: This is the first report of combined-type canine osteosarcoma in the ischium. The case reported here is unusual, as there are few reports of canine osteosarcoma in the pelvic bones, and there is no concrete information regarding its histological appearance. Osteosarcoma is the most common bone neoplasm in dogs, representing up to 80% of the tumours found in such organs. In the present case, the dog was a large young adult with a higher probability of neoplasm development. A cytopathological examination is a diagnostic method with good sensitivity and specificity that can confirm osteosarcomas. However, in this case, the cytological diagnosis, performed without the information from the imaging exam, indicated that it was an undifferentiated sarcoma, given the impossibility of the architectural assessment of the lesion. Biopsy samples sent for histology may not be representative of the entire tumour, leading to misclassification of the histological type. Therefore, the evaluation of fragments from various sites of the lesions is recommended. Regarding the morphology of osteosarcomas, such neoplasms have the osteoblastic, chondroblastic, fibroblastic, telangiectatic, large cell, and poorly differentiated subtypes. With regard to tumours located in the axial skeleton, no studies have assessed the predominance of a particular morphological type, as well as the incidence of combined-type masses in dogs in this particular location. Such neoplasms are locally aggressive and have a high metastatic potential, with the lungs being the main location for implantation of neoplastic cells. There is no proven evidence of the correlation between morphological presentations and the presence of metastases from osteosarcomas in dogs. The histological type is not a predictive factor for the behaviour of the neoplasm. However, the anatomical location is considered as one of the factors with the greatest influence on the prognosis and metastatic potential. Rib masses are associated with a higher rate of metastases compared to others. The definitive diagnosis of osteosarcomas and its correct subclassification are of great importance in the prognosis of affected patients. These require an approach that considers the clinical findings, imaging examinations, and macroscopic and microscopic alterations. Keywords: bone, canine, cytopathology, histopathology, neoplasm.Título: Osteossarcoma pélvico metastático em cão Descritores: canino, citopatologia, histopatologia, neoplasia, osso

Composição química e energética de alimentos para frangos de corte

RESUMO O objetivo deste trabalho foi determinar a composição química e os valores de energia metabolizável aparente (EMA) e metabolizável aparente corrigida pelo balanço de nitrogênio, EMAn, de alguns alimentos para frangos de corte. Foram utilizados 432 pintos de corte macho, de 14 a 24 dias de idade, distribuídos em delineamento inteiramente casualizado com 12 tratamentos (11 alimentos e uma dieta referência), seis repetições e seis aves por unidade experimental. A metodologia utilizada foi da coleta total de excretas. Foram objetos de estudo: Duas farinhas de vísceras (FV), duas farinhas de penas e sangue (FPS), duas farinhas de carnes e ossos (FCO), soja integral tostada (SIT), farelo de soja (FS), quirera de milho (QM), farelo de trigo (FT) e milheto (M). Os valores de proteína bruta e extrato etéreo (%), EMA e EMAn (kcal/kg) foram, respectivamente: 58,84; 12,64; 3705 e 3340 para a FV 1; 57,64; 11,66; 3565 e 3241 para a FV 2; 80,58; 6,79; 3070 e 2740 para a FPS 1; 82,27; 6,38; 3060 e 2703 para a FPS 2; 48,06; 9,60; 1574 e 1283 para a FCO 1; 41,21; 9,08; 1353 e 1088 para a FCO 2; 36,45; 20,78; 3192 e 2989 para a SIT; 46,22; 1,72; 2648 e 2336 para o FS; 8,51; 3,61; 3354 e 3265 para a QM; 17,11; 3,14; 1948 e 1788 para o FT e 12,01; 4,09; 3072 e 2948 para o M. As diferenças observadas na composição química e energética evidenciam a necessidade de pesquisas para atualizar os valores nutricionais dos alimentos

Metastatic Pelvic Osteosarcoma in a Dog

Background: Osteosarcomas are malignant neoplasms of bone tissue, with a high prevalence in dogs, especially in large and giant breeds. More commonly, such alterations affect the appendicular skeleton and, to a lesser extent, the axial skeleton. In order to obtain an accurate diagnosis, it is necessary to combine cytological and histopathological findings with clinical parameters, imaging exams and macroscopic findings. In the present study, we report a rare case of combined-type pelvic osteosarcoma with pulmonary metastasis in a dog.Case: A 5-year-old intact large male dog of mixed breed, was submitted to clinical care because of an increase in volume of the left perineal region.  The cytological evaluation, performed without imaging exams, indicated that it was an undifferentiated sarcoma. An incisional biopsy defined the diagnosis as telangiectatic osteosarcoma, and with progressive clinical worsening, the patient died. Necroscopic examination revealed multiple nodules in the lungs and an irregular mass with a hard to friable consistency. The mass was intensely vascularised and extended craniodorsally from the left ischial tuberosity to the base of the renal fossa. Microscopically, the neoplasm was diagnosed as combined osteosarcoma, consisting of the osteoblastic, chondroblastic, and telangiectatic subtypes. Metastases with a predominance of the chondroblastic subtype were observed in the lungs.Discussion: This is the first report of combined-type canine osteosarcoma in the ischium. The case reported here is unusual, as there are few reports of canine osteosarcoma in the pelvic bones, and there is no concrete information regarding its histological appearance. Osteosarcoma is the most common bone neoplasm in dogs, representing up to 80% of the tumours found in such organs. In the present case, the dog was a large young adult with a higher probability of neoplasm development. A cytopathological examination is a diagnostic method with good sensitivity and specificity that can confirm osteosarcomas. However, in this case, the cytological diagnosis, performed without the information from the imaging exam, indicated that it was an undifferentiated sarcoma, given the impossibility of the architectural assessment of the lesion. Biopsy samples sent for histology may not be representative of the entire tumour, leading to misclassification of the histological type. Therefore, the evaluation of fragments from various sites of the lesions is recommended. Regarding the morphology of osteosarcomas, such neoplasms have the osteoblastic, chondroblastic, fibroblastic, telangiectatic, large cell, and poorly differentiated subtypes. With regard to tumours located in the axial skeleton, no studies have assessed the predominance of a particular morphological type, as well as the incidence of combined-type masses in dogs in this particular location. Such neoplasms are locally aggressive and have a high metastatic potential, with the lungs being the main location for implantation of neoplastic cells. There is no proven evidence of the correlation between morphological presentations and the presence of metastases from osteosarcomas in dogs. The histological type is not a predictive factor for the behaviour of the neoplasm. However, the anatomical location is considered as one of the factors with the greatest influence on the prognosis and metastatic potential. Rib masses are associated with a higher rate of metastases compared to others. The definitive diagnosis of osteosarcomas and its correct subclassification are of great importance in the prognosis of affected patients. These require an approach that considers the clinical findings, imaging examinations, and macroscopic and microscopic alterations. Keywords: bone, canine, cytopathology, histopathology, neoplasm.Título: Osteossarcoma pélvico metastático em cão Descritores: canino, citopatologia, histopatologia, neoplasia, osso

Resumos concluídos - Fisiopatologia

Resumos concluídos - Fisiopatologi

Mídia e política no Brasil: textos e agenda de pesquisa Midia and politics in Brazil: texts and research agenda

Um especialista em estudos de comunicação e um cientista político apresentam conjuntamente um panorama da pesquisa sobre as relações entre os meios de comunicação e os processos políticos no Brasil. Uma agenda de pesquisa é proposta e um elenco de textos nessa área é apresentado.<br>A specialist in communication studies and a political scientist present together a panorama of research on the relations between communication midia and political processes in Brazil A research agenda is proposed and a list of texts in this area is presented

Guidance on mucositis assessment from the MASCC Mucositis Study Group and ISOO: an international Delphi studyResearch in context

Summary: Background: Mucositis is a common and highly impactful side effect of conventional and emerging cancer therapy and thus the subject of intense investigation. Although common practice, mucositis assessment is heterogeneously adopted and poorly guided, impacting evidence synthesis and translation. The Multinational Association of Supportive Care in Cancer (MASCC) Mucositis Study Group (MSG) therefore aimed to establish expert recommendations for how existing mucositis assessment tools should be used, in clinical care and trials contexts, to improve the consistency of mucositis assessment. Methods: This study was conducted over two stages (January 2022–July 2023). The first phase involved a survey to MASCC-MSG members (January 2022–May 2022), capturing current practices, challenges and preferences. These then informed the second phase, in which a set of initial recommendations were prepared and refined using the Delphi method (February 2023–May 2023). Consensus was defined as agreement on a parameter by >80% of respondents. Findings: Seventy-two MASCC-MSG members completed the first phase of the study (37 females, 34 males, mainly oral care specialists). High variability was noted in the use of mucositis assessment tools, with a high reliance on clinician assessment compared to patient reported outcome measures (PROMs, 47% vs 3%, 37% used a combination). The World Health Organization (WHO) and Common Terminology Criteria for Adverse Events (CTCAE) scales were most commonly used to assess mucositis across multiple settings. Initial recommendations were reviewed by experienced MSG members and following two rounds of Delphi survey consensus was achieved in 91 of 100 recommendations. For example, in patients receiving chemotherapy, the recommended tool for clinician assessment in clinical practice is WHO for oral mucositis (89.5% consensus), and WHO or CTCAE for gastrointestinal mucositis (85.7% consensus). The recommended PROM in clinical trials is OMD/WQ for oral mucositis (93.3% consensus), and PRO-CTCAE for gastrointestinal mucositis (83.3% consensus). Interpretation: These new recommendations provide much needed guidance on mucositis assessment and may be applied in both clinical practice and research to streamline comparison and synthesis of global data sets, thus accelerating translation of new knowledge into clinical practice. Funding: No funding was received