Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters

<p>Abstract</p> <p>Background</p> <p>Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from <it>Campylobacter </it>organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions.</p> <p>Results</p> <p>Only <it>C. sputorum </it>biovar sputorum LMG7975 and fecalis LMG8531, LMG8534 and LMG6728 of a total of 204 <it>Campylobacter </it>isolates (n = 56 <it>C. jejuni</it>; n = 11 <it>C. coli</it>; n = 33 <it>C. fetus</it>; n = 43 <it>C. upsaliensis</it>; n = 30 <it>C. hyointestinalis</it>; n = 4 <it>C. sputorum </it>biovar sputorum; n = 5 <it>C. sputorum </it>biovar fecalis; n = 5 <it>C. sputorum </it>biovar paraureolyticus; n = 10 <it>C. concisus</it>; n = 7 <it>C. curvus</it>) were shown to carry IVSs in helix 25 region. <it>C. sputorum </it>biovar fecalis LMG8531 and LMG8534, interestingly, carried two different kinds of the 23S rRNA genes with and without the IVS, respectively. Consequently, in a total of 265 isolates of 269, including 65 <it>C. lari </it>isolates examined previously, the absence of IVSs was identified in the helix 25 region. In the helix 45 region, all the <it>C. hyointestinalis</it>, <it>C. sputorum </it>and <it>C. concisus </it>isolates were shown not to carry any IVSs. However, the 30 of 56 <it>C. jejuni </it>isolates (54%), 5 of 11 <it>C. coli </it>(45%), 25 of 33 <it>C. fetus </it>(76%), 30 of 43 <it>C. upsaliensis </it>(70%) and 6 of 7 <it>C. curvus </it>(90%) were shown to carry IVSs. In <it>C. jejuni </it>and <it>C. upsaliensis </it>isolates, two different kinds of the 23S rRNA genes were also identified to occur with and without IVSs in the helix 45 region, respectively.</p> <p>Conclusions</p> <p>Secondary structure models were also constructed with all the IVSs identified in the present study. In the purified RNA fractions from the isolates which carried the 16S or 23S rRNA genes with the IVSs, no 16S or 23S rRNA was evident, respectively.</p

Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis

<p>Abstract</p> <p>Background</p> <p>Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated three amplicons for each of isolates of <it>Taylorella asinigenitalis </it>(UCD-1^T, UK-1 and UK-2).</p> <p>Findings</p> <p>Following TA cloning and sequencing, the three isolates of <it>T. asinigenitalis </it>were demonstrated to possess three ISR units of different lengths. Although the three corresponding ISRs (A, B and C) were identified to be identical to each other (UK-1 and UK-2 isolates), the ISRs shared approximately 95.3–98.9% nucleotide sequence similarities between the UCD-1^Tand UK-1/-2 isolates. A typical order of two intercistronic tRNA genes (5'-tRNA^Ile-tRNA^Ala-3') with the different nucleotide spacers [44 through 51 base pairs (bp)] in length was identified among the isolates. The consensus sequences of the antiterminators of <b>boxB </b>and <b>boxA </b>were also identified in all ISRs. Thus, three ISRs were identified for each isolate, and therefore, at least three distinctly different ribosomal RNA operons were suggested to occur in the genome of <it>T. asinigenitalis</it>. This was also confirmed by Southern hybridization procedure.</p> <p>Conclusion</p> <p>The present study represents a dendrogram constructed based on the nucleotide sequence data of 16S-23S rDNA ISR for <it>T. asinigenitalis</it>, which may aid in the phylogenetic positioning of <it>T. asinigenitalis </it>within the genus <it>Taylorella</it>, and in the molecular discrimination of <it>T. asinigenitalis</it>.</p

進路選択自己効力とキャリア教育およびキャリア支援プログラムの有用性との関連

本研究は,大学3回生対象のキャリア教育プログラム(career education program:以下,CEP3)や,大学の就職部・キャリアセンターが行ってきたキャリア支援プログラム(careersupport program:以下,CSP)に対して受講生が役に立ったかどうかという捉え方の程度(役立ち度)が進路選択自己効力と関連するのかを明らかにすることを目的とした。計21項目のCEP やCSP に対する役立ち度得点について因子分析を行ったところ,「就職情報調査獲得因子」「就職活動表出因子」「自己分析因子」の3因子が抽出された。各因子の役立ち度得点,および「相談相手の経路数」「アルバイト総継続期間」の5つを説明変数とし,進路選択自己効力尺度得点を目的変数とする重回帰分析を行った結果,「自己分析因子」に関連するプログラムに対して役に立つかどうかという捉え方と進路選択自己効力に関連があることが明らかとなった。これらの結果は,自己分析に関連したプログラムが進路選択自己効力の向上に効果的である可能性を示唆する。さらに,CEP が行われた授業への出席率と進路選択自己効力との違いを検討した結果,CEP を受講することが進路選択自己効力の低下防止に繋がる可能性が示唆された

Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within <it>Campylobacter lari</it>

<p>Abstract</p> <p>Background</p> <p>The combined sequences encoding a partial and putative <it>rpsI </it>open reading frame (ORF), non-coding (NC) region, a putative ORF for the <it>Campylobacter </it>adhesin to fibronectin-like protein (<it>cadF</it>), a putative Cla_0387 ORF, NC region and a partial and putative Cla_0388 ORF, were identified in 16 <it>Campylobacter lari </it>isolates, using two novel degenerate primer pairs. Probable consensus sequence at the -35 and -10 regions were identified in all <it>C. lari </it>isolates, as a promoter.</p> <p>Results</p> <p>Thus, <it>cadF </it>(-like) gene is highly conserved among <it>C. lari </it>organisms. Transcription of the <it>cadF </it>(-like) gene in <it>C. lari </it>cells <it>in vivo </it>was also confirmed and the transcription initiation site was determined. A peptidoglycan-associating alpha-helical motif in the C-terminal regions of some bacterial cell-surface proteins was completely conserved amongst the putative <it>cadF </it>(-like) ORFs from the <it>C. lari </it>isolates.</p> <p>Conclusion</p> <p>The putative <it>cadF </it>(-like) ORFs from all <it>C. lari </it>isolates were nine amino acid larger than those from <it>C. jejuni</it>, and showed amino acid residues 137 -140 of FALG (50% identity), instead of the FRLS residues of the maximal fibronectin-binding activity site demonstrated within <it>C. jejuni </it>CadF. A neighbor joining tree constructed based on <it>cadF </it>(-like) gene sequence information formed a major cluster consisting of <it>C. lari </it>isolates, separating from the other three thermophilic campylobacters.</p