OCORRÊNCIA DE TUMOR VENÉREO TRANSMISSIVEL EM CÃES ATENDIDOS NO HOVET - PA UFRA

The objective was to verify the occurrence of the transmissible venereal tumor in canines attended at (UFRA), Campus Belém / Pará, from March 2016 to September 2019. The information was obtained from the SISVET® program (Veterinary System). A total of 3,714 records were analyzed regarding the animals served and sent to the animal reproduction sector. Of this total, 155 were from dogs diagnosed with transmissible venereal tumor and 8 negative cases, 102 females and 53 males. The data were tabulated in an electronic spreadsheet, submitted to statistical analysis using the SAS® Universty Edition software regarding the frequency of occurrences, the influence of the age group, sex, race, through the Chi-Square test with 5% significance. Of the 155 cases of TVT diagnosed, 102 (65.81%) were in females and 53 (34.19%) males, with prevalence in SRD (72.86%) and in adults (54.19%). As for the site of involvement, 32.26% were in vulva and 20% in the body of the penis. Regarding the neighborhoods, there was a predominance in Guamá with 25 cases (15.59%). Thus, it is concluded that TVT was more frequent in SRD females and in adult dogs. Vulva 50 (32.26%), vagina 46 (29.68%) and penis 31 (20%) were the most affected sites.O objetivo foi verificar a ocorrência do tumor venéreo transmissível em caninos atendidos na (UFRA), Campus Belém/Pará, no período de março de 2016 a setembro de 2019. As informações foram obtidas no programa SISVET® (Sistema Veterinário). Foram analisados 3.714 registros referentes aos animais atendidos e encaminhados ao setor de reprodução animal. Desse total, 155 eram de cães diagnosticados com tumor venéreo transmissível e 8 casos negativos, sendo 102 fêmeas e 53 machos. Os dados foram tabulados em planilha eletrônica, submetidos à análise estatística no software SAS® Universty Edition quanto à frequência de ocorrências, influência da faixa etária, sexo, raça, por meio do teste qui-quadrado com 5% de significância. Dos 155 casos de TVT diagnosticados, 102 (65,81%) eram do sexo feminino e 53 (34,19%) do sexo masculino, com prevalência em SRD (72,86%) e em adultos (54,19%). Quanto ao local de acometimento, 32,26% foram na vulva e 20% no corpo do pênis. Em relação aos bairros, houve predominância no Guamá com 25 casos (15,59%). Assim, conclui-se que o TVT foi mais frequente em fêmeas SRD e em cães adultos. Vulva 50 (32,26%), vagina 46 (29,68%) e pênis 31 (20%) foram os locais mais acometidos

Epidermoid Polycystic Ovaries and Uterine Hypoplasia in a Cow

 Background: Ovarian cysts originate from mature follicles that do not ovulate within the predicted time according to the oestrous cycle. Dermoid cysts are structures lined by keratinized stratified squamous epithelium, without adjacent epidermal structures, filled by keratinic debris and amorphous proteinaceus material. They are small, located below the cortex, near the hilus. There is controversy regarding the teratogenicity of the dermoid cyst. Some authors use the nomenclature of benign cystic ovarian teratoma, others, reports that although the fibrous wall and histological features are similar to teratomas, it is not associated with this germ cell-derived neoplasia.Case: Ovaries and uterus were received from a female, adult bovine, Nelore, from a slaughterhouse. The left ovary measured 6.5x5.0x3.9 cm and weighed 80 g; the right ovary measured 5.7x3.7x3.0 cm and weighed 60 g. Grossly, the ovaries were similar, presenting floating consistency, multilobulated and pointed aspect, and at the cut surface, there was extravasation of mucopurulent content of whitish colour. Regarding to the uterus, macroscopically, the uterine horns were infantile and decrease in the diameter. Fragments of the tissues were collected and fixed in 10% buffered formalin for histopathological examination and the staining of the slides was done with hematoxylin and eosin. Microscopically, in the ovaries, it was observed rare vestigial elements of ovary identifying an atresic follicle. Polycystic formation with cysts exhibiting partial or total coating of a keratinized squamous epithelium was also observed. In the lumen abundant keratinous material was present. The uterine microscopy revealed compact endometrium with areas variably infiltrated by lymphocytes, plasmocytes and occasional neutrophils. Areas devoid of endometrial glands were observed, in addition to other areas with reduced quantity and size. Also, the myoepithelial layer was thin and enlargement of the endometrial vascular space was noted.Discussion: Only one study was found reporting three cases of unilateral dermoid cyst in bovine ovary, also from slaughterhouse. Dermoid cysts appear most often in Zebu cattle. Clinically, these cysts do not necessarily result in infertility and do not interfere in the ovarian functions, due to this, the ovaries are not referred for histopathology examination, which difficult its classification. However, in this study the histological examination showed the absence of follicular development, which characterizes infertility, including endocrine dysfunction. Macroscopically ovarian dermoid cysts are similar to abscesses. It has a viscous or milky content inside and, at the cut surface, usually the content project to the surface. Histologically, there are dilated cysts containing material in the lumen at various stages of keratinization conferring a lamellar aspect and a keratinized stratified squamous epithelium. In this study, we observed pseudostratified epithelial lining containing cilia in addition to lumen keratin content, without follicles or adnexal structures, corroborating the findings in the literature. Also, it was observed uterine hypoplasia, which occurs due to failure of the development of the paramesonephric ducts during embryonic development. Ovarian dermoid cysts associated with uterine hypoplasia are infrequent in animals and, clinically, may not present changes in fertility and ovarian function

Polimorfismos no gene da osteopontina e suas associações com a fertilidade de búfalos na Amazônia

Because the osteopontin gene can influence the fertility of water buffaloes, the aim of this work was to identify polymorphisms in this gene and associate them with fertility parameters of animals kept under extensive grazing. We used 306 male buffaloes older than 18 months, bred on two farms, one in the state of Amapá and the other in the state of Pará. We identified three SNP for the region amplified by the primer OS4 (5`UTR) and four SNP polymorphisms for the region amplified by the primer OS9 (exon 5 to exon 6). The polymorphisms were in positions 1478, 1513 and 1611 in the region amplified by OS4 and positions 6690, 6737, 6925 and 6952 in the region amplified by OS9. We calculated correlations with the traits scrotal circumference and volume and sperm motility, concentration and pathology. There were SNPs for all the traits studied at 5% significance: for circumference, SNP 6690; for volume, SNP 6737; for concentration, SNP 6690; for motility, SNP 6690; and for pathology, SNP 6690. Therefore, SNP 6690 was related to four traits. The AA genotype of SNP 6690 presented the highest averages for scrotal circumference, sperm concentration and motility and the lowest total number of pathologies. However, for the scrotal volume trait, the animals with the largest volume were correlated with the presence of the GG genotype of SNP 6737. This indicates that the osteopontin gene is important because it can have a substantial influence on the reproductive traits of male buffaloes.Este trabalho teve como objetivo identificar polimorfismos neste gene e associar os mesmos aos parâmetros de fertilidade de búfalos criados de forma extensiva. Foram utilizados 306 machos bubalinos, acima de 18 meses de idade, criados em duas propriedades, uma no estado do Amapá e outra no estado do Pará. Os resultados obtidos para a extração de DNA através de pêlos foram de excelente quantidade, com concentração maior que 50ng/l. Ambos pares de primers amplificaram as regiões específicas do gene da osteopontina. Foram identificados 3 polimorfismos do tipo SNP para a região amplificada pelo primer OS4 (5`UTR) e 4 polimorfismos do tipo SNP para a região amplificada pelo primer OS9 (éxon 5 ao éxon 6). Os polimorfismos foram nas posições 1478, 1513 e 1611 na região amplificada pelo OS4 e nas posições 6690, 6737, 6925 e 6952 na região amplificada pelo OS9. Para todas as características estudadas foram encontrados SNPs significativos a 5%. Para circunferência escrotal, o SNP 6690; para volume testicular, o SNP 6737; para a concentração, o SNP 6690; para motilidade, o SNP 6690 e para patologia espermática, o SNP 6690. Estando os polimorfismos do SNP 6690 relacionado com quatro características. Para a característica CE o genótipo AA foi associado (P<0,05) com os animais que apresentaram maior média de circunferência escrotal, maior média de concentração espermática, maior média de motilidade e menor número de patologias totais. Entretanto, para a característica volume testicular, os animais que apresentaram maior volume estão correlacionados com a presença do genótipo GG, para o SNP 6737. Isso indica que o gene da osteopontina pode influenciar as características reprodutivas em machos bubalinos

Leptospirosis, bovine viral diarrhea and infectious bovine rhinotracheitis: prevalence in Colombian cattle and buffaloes

One of the limiting factors of productive efficiency in cattle and buffalo herds is related to the high prevalence of infectious diseases which affect reproduction. The aim of this study was to determine the prevalence of anti-Leptospira antibodies for bovine viral diarrhea virus (BVDV) and bovine herpesvirus type 1 (BoHV-1) in bovine and buffalo herds in Colombia. Blood serum samples were collected from 1100 buffaloes and 1000 cattle. The ELISA technique was used to detect antibodies against BVDV and BoHV-1, and the microscopic agglutination technique to detect anti-Leptospira antibodies. The prevalence of anti-Leptospira antibodies and of BVDV and BoHV-1 in bovine samples was observed in 16, 39.7, and 65% of animals, respectively, while the positivity in samples for the same antibodies in buffalos was detected in 18.7, 27.5 and 51.5%, respectively. Exposure of cattle and buffaloes to BoHV-1 was positively associated with age, higher prevalence rates were observed in older ages. Seropositivity of cattle for BVDV and BoHV-1 was higher in male animals. Activities such as embryo transfer, milking, and needle reuses, as well as the presence of cats and rodents are factors which favor positivity of the herd for BVDV and BoHV-1.Incluye referencias bibliográficas

Diagnóstico investigativo de patologias no trato genital de búfalas criadas extensivamente, no estado do Amapá, Amazônia, Brasil

RESUMO. Ribeiro H.F.L., Mourão F.R.P., Monteiro F.J.C., Rolim Filho S.T. & Vale W.G. Diagnosis of investigative pathology in the genital tract of buffaloes raised extensively in the state of Amapá, Amazon, Brazil. [Diagnóstico investigativo de patologias no trato genital de búfalas criadas extensivamente, no estado do Amapá, Amazônia, Brasil.] Revista Brasileira de Medicina Veterinária, 38(4):358-364, 2016. Programa de Pós-Graduação em Ciência Animal, Universidade Federal do Pará, Rua Augusto Corrêa 1, Campus Universitário do Guamá, Belém, PA 66075-110, Brazil. E-mail: [email protected] A investigação nos 1000 tratos genitais foi realizada em dois matadouros no Município de Macapá, Estado do Amapá e consistiu em exames macroscópicos e microscópicos dos ovários, tubas uterina, útero e cérvice. As observações de cada genitália foram documentadas em fichas especiais, com anotações detalhadas de todas as alterações diagnosticadas, com as genitálias foram classificadas em: gestante e não gestante, com ou sem patologias, respectivamente e coletados fragmentos patológicos e fixados em formol tamponado a 10%, para a realização de exames histopatológicos. Do total investigado 661 (66,10%) tratos genitais estavam gestantes e 339 (33,90%) não gestantes. Dos 1000 tratos genitais, 840 (84,00%) apresentavam-se normais, e, destes, 575 (57,50%) gestantes, e as demais 265 (26,5%) não gestantes. Entre os 661 tratos genitais gestantes, 412 (62,3%) encontravam-se no corno direito e 249 (37,7%) no corno esquerdo. 160 tratos genitais (16,0%) apresentavam patologias, sendo que 86 (8,6%) estavam gestantes e 74 (7,4%) não gestantes. Dentre as 160 (16,00%) patologias diagnosticadas, as mais prevalentes foram as 114 (11,4%) na tuba uterina, seguida pelas 30 (3,0%) no ovário e 16 (1,6%) no útero ou cérvice. As alterações de maior prevalência foram as aderências da trompa uterina, a distrofia ovariana e as endometrites

REPRODUCTIVE CHARACTERISTICS OF BUFFALOES RAISED IN FLOODPLAIN AND MAIN LAND IN PARÁ STATE. I. AGE AT FIRST CALVING, INTER CALVING INTERVAL, CALVING SEASON, REPRODUCTIVE EFFICIENCY AND PREGNANCY RATES. DESEMPENHO REPRODUTIVO DE FÊMEAS BUBALINAS CRIADAS EM SISTEMA MISTO (VÁRZEA E PASTAGEM ARTIFICIAL) NO ESTADO DO PARÁ. I. IDADE A PRIMEIRA CRIA, INTERVALO ENTRE PARTOS, EPOCA DE PARIÇÃO, EFICIÊNCIA REPRODUTIVA E TAXA DE PRENHEZ

The objective of this work was to evaluate the reproductive efficiency of a buffaloes flock raised in a productive system of river and main land/artificial pasture in Belém County, Pará, state. Data on 770 female buffaloes in a total of 2115 parturition registers have been analyzed. From this herd 57 animals were submitted to gynecological examination and selected according to the body score condition (BSC) in a scale of (1-5) and inseminated in a program of fixed time for artificial insemination (FTAI). The animals were divided in two groups, the Group 1 (G1) with 31 animals and Group 2 (G2) with 26 animals, both synchronized by the protocol Ovsynch and inseminated in fixed time with 00:00h and 24:00h after of GnRH. The age for first parturition (AFP) for the whole herd was  39.52±7.54 months. The AFP related to the natural mating (NM), artificial insemination (AI) and fixed timed artificial insemination (FTAI), was of 39.5±6.58; 39.6±9.85 and 36.6±7.12 months, respectively. Considering the seasonal rain annual distribution, the AFP and the calving interval (CI) was 39.40±7.29 and 38.74±7.76,  17.09±4.71 and 16.54±3.74 months, for rainy and less rainy period, respectively,  with a concentration between March to August through 1974 and 2005. The overall average for CI 16.54±3.74 meses months. Moreover, according to the reproductive system employed, natural mated (NM), AI and FTAI was17.31, 18.43 and 20.79 months, respectively. The overall reproductive efficiency (RE) was of 0.67±0.13, and for the animals submitted to the NM, AI and FTAI was 0.69; 0.56; 0.63 respectively. The pregnancy rates obtained for each group, taking in account the moment or time for artificial insemination and the application of GnRH was of 25.81%(08/31) in the animals inseminated on 00:00h and of 30,77 % (08/26) in the inseminated on 24:00h.&lt;br /&gt;&lt;br /&gt;KEY WORDS: Buffaloes, calving interval, reproductive efficiency.&lt;br /&gt;&lt;br /&gt; O objetivo do presente estudo foi avaliar a eficiência reprodutiva de bubalinos criados em sistema misto (várzea e terra firme/pastagem artificial) em uma fazenda experimental no município de Belém, Estado do Pará. Analisaram-se 770 registros de fêmeas bubalinas, entre 1974 a 2005, totalizando 2.115 registros de partos. Selecionaram-se 57 búfalas, para a inseminação artificial em tempo fixo (IATF), as quais foram divididas em dois grupos – grupo 1 (G1) com 31 animais e grupo 2 (G2) com 26 –, submetidas à sincronização pelo protocolo Ovsynch e inseminadas com 00:00h (G1) e 24:00h (G2), após a última aplicação de GnRH. A média de idade ao primeiro parto (IPP) do rebanho foi de 39,52±7,54. As médias e os desvios-padrão para idade ao primeiro parto (IPP) e sua relação com os sistemas de monta natural (MN), inseminação artificial (IA) e inseminação artificial em tempo fixo (IATF) foram de 39,5±6,58; 39,6±9,85 e 36,6±7,12 meses respectivamente. Considerando-se o período climático, na época mais chuvosa, as médias da IPP e do intervalo entre partos (IEP) foram de 39,40±7,29 e 17,09±4,71, e na menos chuvosa, de 38,74±7,76 e 18,89±4,27 meses, respectivamente. A média do IEP do rebanho foi de 16,54±3,74 meses. Em relação ao sistema de reprodução, essa média variou para 17,31 (MN), 18,43 (IA), e 20,79 (IATF) meses. Houve uma maior concentração dos partos entre os meses de março e agosto. A média e desvio-padrão da eficiência reprodutiva (ER) do rebanho foi de 0.67±0,13 e para os sistemas de MN, IA e IATF, apresentando médias de 0,69; 0,56; 0,63, respectivamente. A taxa de prenhez foi 25,81% (08/31) nos animais inseminados em 00:00h e de 30,77 % (08/26) nos inseminados com 24:00h após a aplicação de GnRH.&lt;br /&gt;&lt;br /&gt;PALAVRAS-CHAVES: Bubalinos, eficiência reprodutiva, intervalo entre partos.&lt;br /&gt

NEOTROPICAL ALIEN MAMMALS: a data set of occurrence and abundance of alien mammals in the Neotropics

Biological invasion is one of the main threats to native biodiversity. For a species to become invasive, it must be voluntarily or involuntarily introduced by humans into a nonnative habitat. Mammals were among first taxa to be introduced worldwide for game, meat, and labor, yet the number of species introduced in the Neotropics remains unknown. In this data set, we make available occurrence and abundance data on mammal species that (1) transposed a geographical barrier and (2) were voluntarily or involuntarily introduced by humans into the Neotropics. Our data set is composed of 73,738 historical and current georeferenced records on alien mammal species of which around 96% correspond to occurrence data on 77 species belonging to eight orders and 26 families. Data cover 26 continental countries in the Neotropics, ranging from Mexico and its frontier regions (southern Florida and coastal-central Florida in the southeast United States) to Argentina, Paraguay, Chile, and Uruguay, and the 13 countries of Caribbean islands. Our data set also includes neotropical species (e.g., Callithrix sp., Myocastor coypus, Nasua nasua) considered alien in particular areas of Neotropics. The most numerous species in terms of records are from Bos sp. (n = 37,782), Sus scrofa (n = 6,730), and Canis familiaris (n = 10,084); 17 species were represented by only one record (e.g., Syncerus caffer, Cervus timorensis, Cervus unicolor, Canis latrans). Primates have the highest number of species in the data set (n = 20 species), partly because of uncertainties regarding taxonomic identification of the genera Callithrix, which includes the species Callithrix aurita, Callithrix flaviceps, Callithrix geoffroyi, Callithrix jacchus, Callithrix kuhlii, Callithrix penicillata, and their hybrids. This unique data set will be a valuable source of information on invasion risk assessments, biodiversity redistribution and conservation-related research. There are no copyright restrictions. Please cite this data paper when using the data in publications. We also request that researchers and teachers inform us on how they are using the data