374 research outputs found
Ag(I)-Catalyzed Aminofluorination of Alkynes: Efficient Synthesis of 4‑Fluoroisoquinolines and 4‑Fluoropyrrolo[α]isoquinolines
A silver-catalyzed intramolecular oxidative aminofluorination of alkynes has been developed by using NFSI as a fluorinating reagent. This reaction represents an efficient method for the synthesis of various 4-fluoroisoquinolines and 4-fluoropyrrolo[α]isoquinolines
Ag(I)-Catalyzed Aminofluorination of Alkynes: Efficient Synthesis of 4‑Fluoroisoquinolines and 4‑Fluoropyrrolo[α]isoquinolines
A silver-catalyzed intramolecular oxidative aminofluorination of alkynes has been developed by using NFSI as a fluorinating reagent. This reaction represents an efficient method for the synthesis of various 4-fluoroisoquinolines and 4-fluoropyrrolo[α]isoquinolines
Survival of wildtype and <i>recA</i> cells to MNNG in L broth and glucose minimal medium.
<p>Logarithmic phase wildtype (filled symbols) or <i>recA</i> (open symbols) cells growing at 37°C in L broth (squares) or glucose minimal medium (circles) were exposed to various concentrations of MNNG for 30 min, and then diluted and plated on L medium to determine survival.</p
Survival of <i>lig-7</i> (Ts) cells to BLM in L broth and glucose minimal medium.
<p>Logarithmic phase <i>lig-7</i> (Ts) cells in L broth or glucose minimal medium at 30°C (permissive) or 42°C (non-permissive) were exposed to various concentrations of BLM for 30 min, and then diluted and plated on L medium to determine survival. The plates were incubated at 30°C.</p
Survival of wildtype and <i>recA</i> cells to high concentrations of BLM.
<p>Logarithmic phase cells growing at 37°C in glucose minimal medium were exposed to various concentrations of BLM for 30 min, and then diluted and plated on L medium to determine survival. The BLM concentrations used in the Figure are ten-times greater than those used in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033256#pone-0033256-g001" target="_blank">Figures 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033256#pone-0033256-g002" target="_blank">2</a>.</p
Survival of <i>E. coli</i> strains after exposure to BLM.
<p>Cells in the logarithmic phase of growth in L broth at 37°C were exposed to various concentrations of BLM for 30 min, and then diluted and plated on L medium to determine survival.</p
Survival of wildtype and <i>recA</i> cells to BLM in L broth and glucose minimal medium.
<p>Logarithmic phase cells growing at 37°C in L broth or glucose minimal medium were exposed to various concentrations of BLM for 30 min, and then diluted and plated on L medium to determine survival.</p
Some of the <i>E. coli</i> K-12 strains used in this study.
<p>Some of the <i>E. coli</i> K-12 strains used in this study.</p
Pulse field gel electrophoresis of DNA from cells exposed, or not, to BLM.
<p>(A). Wildtype (lanes 1–4) or <i>recBCD</i> strains (lanes 5–8) growing in L broth were exposed to 0, 0.175, 0.35, and 0.70 µM BLM (lanes 1–4, 5–8) for 30 min before harvesting and processing. (B). The same as in A except that cells were cultivated in glycerol minimal medium.</p
Increase in gene expression after challenge with BLM.
<p>The <i>lexA</i> and <i>dinG</i> genes are included in this table because they are part of the SOS regulon and close to the cut-off value of 2.0.</p
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