Transformation of Inorganic Sulfur into Organic Sulfur: A Novel Photoluminescent 3-D Polymeric Complex Involving Ligands in Situ Formation

The reaction of CuSCN with acetonitrile and methanol under solvothermal conditions yielded a novel 3-D polymeric photoluminescent complex containing dodecanuclear copper(I) clusters with methyl mercaptide. The synthesis involves in situ generation of ligands, which provides a model reaction to simulate the transformation of inorganic sulfur into organic sulfur under geothermic conditions

Transformation of Inorganic Sulfur into Organic Sulfur: A Novel Photoluminescent 3-D Polymeric Complex Involving Ligands in Situ Formation

The reaction of CuSCN with acetonitrile and methanol under solvothermal conditions yielded a novel 3-D polymeric photoluminescent complex containing dodecanuclear copper(I) clusters with methyl mercaptide. The synthesis involves in situ generation of ligands, which provides a model reaction to simulate the transformation of inorganic sulfur into organic sulfur under geothermic conditions

MOESM1 of Yy1 regulates Senp1 contributing to AMPA receptor GluR1 expression following neuronal depolarization

Additional file 1: Figure S1. Membrane depolarization represses the expression of Senp1 and Tet1. (a) The relative mRNA levels of Bdnf IV in cortical neurons was determined by qRT-PCR following treatment with vehicle (Control), 60 mM KCl for 2 hrs, or 24 hrs. (b) Western blot analysis of phosphorylated Creb level following neuronal depolarization in cortical neurons. Total proteins were extracted from cortical neurons after 2 hr treatment with 60 mM KCl and vehicle. Actin was used as loading control. (c-d) The expression of indicated genes in cortical neurons were determined by qRT-PCR following treatment with vehicle, 60 mM KCl for 2 hrs (c), or 24 hrs (d). Only Senp1 and Tet1 were significantly reduced by neuronal activity. Graphs indicate three independent biological replicates. Error bars represent one standard deviation from the mean. *(p < 0.05). p value was determined using two-tailed unpaired t test. (e) Western blot analysis of Senp1 and Yy1 level after treatment with TTX and KCl in cortical neurons. Total proteins were extracted from cortical neurons after 2 hr treatment with 60 mM KCl, 1 μM TTX and vehicle. Actin was used as loading control

Exciton Coupling Analysis and Enolization Monitoring by Vibrational Circular Dichroism Spectra of Camphor Diketones

The keto–enol tautomerization of (1<i>R</i>,3<i>R</i>,4<i>R</i>)-(+)-3-benzoylcamphor in solution was traced by the infrared (IR) and vibrational circular dichroism (VCD) spectra, reflecting the Boltzmann populations of the isomers. To investigate the exciton coupling of the carbonyl vibrations in the region 1800–1600 cm^–1, VCD spectra of a series of camphor derived β-diketones were analyzed with the support of density functional theory (DFT) calculations. The results confirm the importance of the exciton chirality for VCD and manifest that the VCD spectroscopy is a convenient technique to investigate the keto–enol tautomerization equilibria in chiral diketones

MOESM2 of Yy1 regulates Senp1 contributing to AMPA receptor GluR1 expression following neuronal depolarization

Additional file 2: Figure S2. Depletion of Yy1 reduces surface GluR1 in primary cortical neurons. (a) Immunostaining of surface GluR1 in shRNA transfected cells. Primary cortical neurons were transfected with shRNA Control (shCtrl), shYy1–2, or shYy1–3. GFP included in the shRNA vector tracks the transfected cells. Scale bar: 25 μM. (b) Quantification of surface GluR1 level in control and Yy1 depletion neurons. The mean intensity of GluR1 signals was determined using Image J software. *** (p < 0.0001). p value was determined using two-tailed unpaired t test

Transformation of Inorganic Sulfur into Organic Sulfur: A Novel Photoluminescent 3-D Polymeric Complex Involving Ligands in Situ Formation

The reaction of CuSCN with acetonitrile and methanol under solvothermal conditions yielded a novel 3-D polymeric photoluminescent complex containing dodecanuclear copper(I) clusters with methyl mercaptide. The synthesis involves in situ generation of ligands, which provides a model reaction to simulate the transformation of inorganic sulfur into organic sulfur under geothermic conditions

Resolving Resonant Electronic States in Chiral Metal Complexes by Raman Optical Activity Spectroscopy

Chiral metal complexes exhibit rich photophysical properties and are important for applications ranging from biosensing to photocatalysis. We present a combined experimental and computational approach leading to information about energies and transition moments of excited electronic states, documented on two chiral metal complexes. The experimental protocol for measurement of the resonance Raman optical activity comprises multiple techniques, i.e., absorption, circular dichroism, and polarized and differential Raman scattering. An accurate formula for subtraction of the interfering circular dichroism/polarized Raman scattering effect is given. An analysis of the spectra based on density functional theory calculations unveils the geometric and electronic structures of the molecules. Such insight into molecular electronic states of chromophores may be useful for understanding and tuning photochemical properties of metal-containing complexes, biomolecules, and supramolecules

High-Throughput Superoxide Anion Radical Scavenging Capacity Assay

A high-throughput superoxide anion radical (O2•–) scavenging capacity assay based on the xanthine oxidase/xanthine reaction system was developed and validated in the present study. The reaction conditions including detection wavelength, concentrations of reactant components, reaction temperature, reaction time, pH, terminator reagent, and sample dissolving solvents were optimized. The accuracy and reliability of the assay were assessed by evaluation of linearity (r2 = 0 .9513–0.9957), precision (intraday RSD 1.13–4.05% and interday RSD 2.13–5.62%), accuracy (95.64–97.42% recovery), and stability (RSD 2.62–6.19%), as well as comparison with the conventional colorimetric method. The EC50 values obtained by the current method and the conventional assay were highly correlated (r > 0.99). This high-throughput O2•– scavenging assay may be used for screening and estimating potential superoxide anion radical (O2•–) scavengers, especially food extracts and natural products with a very small amount of test material

Resolving Resonant Electronic States in Chiral Metal Complexes by Raman Optical Activity Spectroscopy

Chiral metal complexes exhibit rich photophysical properties and are important for applications ranging from biosensing to photocatalysis. We present a combined experimental and computational approach leading to information about energies and transition moments of excited electronic states, documented on two chiral metal complexes. The experimental protocol for measurement of the resonance Raman optical activity comprises multiple techniques, i.e., absorption, circular dichroism, and polarized and differential Raman scattering. An accurate formula for subtraction of the interfering circular dichroism/polarized Raman scattering effect is given. An analysis of the spectra based on density functional theory calculations unveils the geometric and electronic structures of the molecules. Such insight into molecular electronic states of chromophores may be useful for understanding and tuning photochemical properties of metal-containing complexes, biomolecules, and supramolecules