Conformational and thermodynamic hallmarks of DNA operator site specificity in the copper sensitive operon repressor from Streptomyces lividans

Metal ion homeostasis in bacteria relies on metalloregulatory proteins to upregulate metal resistance genes and enable the organism to preclude metal toxicity. The copper sensitive operon repressor (CsoR) family is widely distributed in bacteria and controls the expression of copper efflux systems. CsoR operator sites consist of G-tract containing pseudopalindromes of which the mechanism of operator binding is poorly understood. Here, we use a structurally characterized CsoR from Streptomyces lividans (CsoRSl) together with three specific operator targets to reveal the salient features pertaining to the mechanism of DNA binding. We reveal that CsoRSl binds to its operator site through a 2-fold axis of symmetry centred on a conserved 5′-TAC/GTA-3′ inverted repeat. Operator recognition is stringently dependent not only on electropositive residues but also on a conserved polar glutamine residue. Thermodynamic and circular dichroic signatures of the CsoRSl-DNA interaction suggest selectivity towards the A-DNA-like topology of the G-tracts at the operator site. Such properties are enhanced on protein binding thus enabling the symmetrical binding of two CsoRSl tetramers. Finally, differential binding modes may exist in operator sites having more than one 5′-TAC/GTA-3′ inverted repeat with implications in vivo for a mechanism of modular control. © 2013 The Author(s)

Disk wind feedback from high-mass protostars

We perform a sequence of 3D magnetohydrodynamic (MHD) simulations of the outflow-core interaction for a massive protostar forming via collapse of an initial cloud core of $60~{M_\odot}$. This allows us to characterize the properties of disk wind driven outflows from massive protostars, which can allow testing of different massive star formation theories. It also enables us to assess quantitatively the impact of outflow feedback on protostellar core morphology and overall star formation efficiency. We find that the opening angle of the flow increases with increasing protostellar mass, in agreement with a simple semi-analytic model. Once the protostar reaches $\sim24~{M_\odot}$ the outflow's opening angle is so wide that it has blown away most of the envelope, thereby nearly ending its own accretion. We thus find an overall star formation efficiency of $\sim50\%$, similar to that expected from low-mass protostellar cores. Our simulation results therefore indicate that the MHD disk wind outflow is the dominant feedback mechanism for helping to shape the stellar initial mass function from a given prestellar core mass function.Comment: Accepted for publication in Ap

Talking About Motherhood Matters: Articulation of Population Policies through National Day Rally Speeches in Singapore

Women’s reproductive rights have always been a site of contestation. The central question this paper seeks to answer is how motherhood is constructed through the repetition of population policies by government officials and how this articulation becomes the script through which motherhood is in turn performed. This paper examines the rhetorical construction of what it means to be a mother in Singapore through the analysis of National Day Rally Speeches. Two themes emerged from this analysis: (1) Motherhood is an expensive experience and, (2) Motherhood required lifestyle changes. By unwittingly painting motherhood as negative experiences, population policies in Singapore could not achieve its goals. Hence, the articulation of population policies should also be considered in order to give women a positive script to performance motherhood

A heterotic sigma model with novel target geometry

We construct a (1,2) heterotic sigma model whose target space geometry consists of a transitive Lie algebroid with complex structure on a Kaehler manifold. We show that, under certain geometrical and topological conditions, there are two distinguished topological half--twists of the heterotic sigma model leading to A and B type half--topological models. Each of these models is characterized by the usual topological BRST operator, stemming from the heterotic (0,2) supersymmetry, and a second BRST operator anticommuting with the former, originating from the (1,0) supersymmetry. These BRST operators combined in a certain way provide each half--topological model with two inequivalent BRST structures and, correspondingly, two distinct perturbative chiral algebras and chiral rings. The latter are studied in detail and characterized geometrically in terms of Lie algebroid cohomology in the quasiclassical limit.Comment: 83 pages, no figures, 2 references adde

Seismological support for the metastable superplume model, sharp features, and phase changes within the lower mantle

Recently, a metastable thermal-chemical convection model was proposed to explain the African Superplume. Its bulk tabular shape remains relatively stable while its interior undergoes significant stirring with low-velocity conduits along its edges and down-welling near the middle. Here, we perform a mapping of chemistry and temperature into P and S velocity variations and replace a seismically derived structure with this hybrid model. Synthetic seismogram sections generated for this 2D model are then compared directly with corresponding seismic observations of P (P, PCP, and PKP) and S (S, SCS, and SKS) phases. These results explain the anticorrelation between the bulk velocity and shear velocity and the sharpness and level of SKS travel time delays. In addition, we present evidence for the existence of a D" triplication (a putative phase change) beneath the down-welling structure

Astrochemical confirmation of the rapid evolution of massive YSOs and explanation for the inferred ages of hot cores

Aims. To understand the roles of infall and protostellar evolution on the envelopes of massive young stellar objects (YSOs). Methods. The chemical evolution of gas and dust is traced, including infall and realistic source evolution. The temperatures are determined self-consistently. Both ad/desorption of ices using recent laboratory temperature-programmed-desorption measurements are included. Results. The observed water abundance jump near 100 K is reproduced by an evaporation front which moves outward as the luminosity increases. Ion-molecule reactions produce water below 100 K. The age of the source is constrained to t \~ 8 +/- 4 x 10^4 yrs since YSO formation. It is shown that the chemical age-dating of hot cores at ~ few x 10^3 - 10^4 yr and the disappearance of hot cores on a timescale of ~ 10^5 yr is a natural consequence of infall in a dynamic envelope and protostellar evolution. Dynamical structures of ~ 350AU such as disks should contain most of the complex second generation species. The assumed order of desorption kinetics does not affect these results.Comment: Accepted by A&A Letters; 4 pages, 5 figure