Proses Transesterifikasi Biji Minyak Jarak dengan Bantuan Enzim Lipase sebagai Penghasil Biodiesel

The investigation was carried out to prevent the scarce of fosil based fuel. In this investigation used seed of castor oil plan (Jathropha curcas L.) as natural row material to make castor oils and converted to biodiesel so that it's friendly to the biological environment. Castor oils produced by extraction process with soxhletation methods used n-hexane solvent. The result of extraction then converted to biodiesel by transesterification process at optimum temperature 40o C with methanol and catalyzed with lipase enzyme. Free fatty acid analysis and total fat analysis was done to the result product, and then biodiesel converted value as 88.67% gotten. Some characteristics analysis of biodiesel castor oils like density, viscosity, pouring point, flaming point, and caloric value according ASTM D6751. The result of investigation showed castor oils biodiesel can be used as diesel fuel

Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance

This study was intended to obtain white rust (Puccinia horiana) disease resistance Chrysanthemum transformed with wasabi defensin gene through mediation of Agrobacterium tumefaciens from three explant sources, i.e., leaf, lateral shoot bud, and internode. Observations were made on transformation efficiency, PCR analysis, in vitro and ex vitro disease resistance tests. Results showed that efficiency of transgenic callus and shoot regeneration was found both highest from lateral shoot buds (57.5% and 50.0%, respectively). PCR analysis showed that three putative transgenic plantlets from lateral shoot buds and one from leaf explant were putative transgenic carrying the wasabi, hpt, and nptII genes. Rooting test showed that the highest number of rooted plants was found in treatment of hygromycin (Hg) 25 mg L-1 (81%) and lowest was in treatment combination of kanamycin (Km) 50 mg L-1 + Hg 25 mg L-1 (25%). In vitro disease resistance test with sorus inoculation of P. horiana, directly on the leaves, resulted in 20 resistant plants out of 30 putative transgenic plants (66.67%). Ex vitro testing on adult plants of the same samples in a confined closed greenhouse (CGH) resulted in average of 80% transgenic Chrysanthemum plants were resistant, whereas in control plants caused white rust disease symptom