Hardware-Oriented Algorithm for Human Detection using GMM-MRCoHOG Features

In this research, we focus on Gaussian mixture model-multiresolution co-occurrence histograms of oriented gradients (GMM-MRCoHOG) features using luminance gradients in images and propose a hardware-oriented algorithm of GMM-MRCoHOG to implement it on a field programmable gate array (FPGA). The proposed method simplifies the calculation of luminance gradients, which is a high-cost operation in the conventional algorithm, by using lookup tables to reduce the circuit size. We also designed a human-detection digital architecture of the proposed algorithm for FPGA implementation using high-level synthesis. The verification results showed that the processing speed of the proposed architecture was approximately 123 times faster than that of the FPGA implementation of VGG-16.17th International Joint Conference on Computer Vision Theory and Applications (VISAPP 2022), February 6-8, 2022, Online Streamin

Hibikino-Musashi@Home 2023 Team Description Paper

This paper describes an overview of the techniques of Hibikino-Musashi@Home, which intends to participate in the domestic standard platform league. The team has developed a dataset generator for the training of a robot vision system and an open-source development environment running on a human support robot simulator. The robot system comprises self-developed libraries including those for motion synthesis and open-source software works on the robot operating system. The team aims to realize a home service robot that assists humans in a home, and continuously attend the competition to evaluate the developed system. The brain-inspired artificial intelligence system is also proposed for service robots which are expected to work in a real home environment

Crystal Structure of an Archaeal Tyrosyl-tRNA Synthetase Bound to Photocaged L-Tyrosine and Its Potential Application to Time-Resolved X-ray Crystallography

Genetically encoded caged amino acids can be used to control the dynamics of protein activities and cellular localization in response to external cues. In the present study, we revealed the structural basis for the recognition of O-(2-nitrobenzyl)-L-tyrosine (oNBTyr) by its specific variant of Methanocaldococcus jannaschii tyrosyl-tRNA synthetase (oNBTyrRS), and then demonstrated its potential availability for time-resolved X-ray crystallography. The substrate-bound crystal structure of oNBTyrRS at a 2.79 Å resolution indicated that the replacement of tyrosine and leucine at positions 32 and 65 by glycine (Tyr32Gly and Leu65Gly, respectively) and Asp158Ser created sufficient space for entry of the bulky substitute into the amino acid binding pocket, while Glu in place of Leu162 formed a hydrogen bond with the nitro moiety of oNBTyr. We also produced an oNBTyr-containing lysozyme through a cell-free protein synthesis system derived from the Escherichia coli B95. ΔA strain with the UAG codon reassigned to the nonnatural amino acid. Another crystallographic study of the caged protein showed that the site-specifically incorporated oNBTyr was degraded to tyrosine by light irradiation of the crystals. Thus, cell-free protein synthesis of caged proteins with oNBTyr could facilitate time-resolved structural analysis of proteins, including medically important membrane proteins

Hardware-oriented Algorithm for Human Detection using GMM-MRCoHOG Features

In this research, we focus on Gaussian mixture model-multiresolution co-occurrence histograms of oriented gradients (GMM-MRCoHOG) features using luminance gradients in images and propose a hardware-oriented algorithm of GMM-MRCoHOG to implement it on a field programmable gate array (FPGA). The proposed method simplifies the calculation of luminance gradients, which is a high-cost operation in the conventional algorithm, by using lookup tables to reduce the circuit size. We also designed a human-detection digital architecture of the proposed algorithm for FPGA implementation using high-level synthesis. The verification results showed that the processing speed of the proposed architecture was approximately 123 times faster than that of the FPGA implementation of VGG-16.17th International Conference on Computer Vision Theory and Applications, 6-8 February, 2022, Online Streamin

Structure of a putative trans-editing enzyme for prolyl-tRNA synthetase from Aeropyrum pernix K1 at 1.7 Å resolution

The three-dimensional structure of the APE2540 protein from A. pernix K1 has been determined by the multiple anomalous dispersion method at 1.7 Å resolution. The structure includes two monomers in the asymmetric unit and shares structural similarity with the YbaK protein or cysteinyl-tRNAPro deacylase from H. influenzae