Predicting Degradation Characteristics from Chemical Composition and Soluble Fraction of Poor Quality Roughage Diets

This study was conducted to explore the feasibility of predicting quality from selected chemical and degradability measurements for two browses (Alchornea cordifolia (A), Ficus coapensis (F), and two crop residues (maize (M) and rice stover (R.) and their mixtures; Degradation characteristics were determined in the rumen of three West African Dwarf (WAD) goats, three WAD sheep and three White Fulani steers. Each animal was fitted with a permanent ruminal canulae, and was routinely fed on Panicum maximum and equal amount officus and alchornea at a ratio of 70:30 respectively (dry matter basis). The DM disappearance values were fitted to the exponential equation P = a + b (l-e*1) where P is amount degradedat time (t) as the soluble fraction, b is the potentially degraded fraction, and c is the rate at which b degrades. Effective degradability (ED) was also determined. The ED of the feeds and their mixtures varied from 256 to 373 (g/kg). The experimentally determined values were compared, to calculate values based on predictions from individual chemical components (separately or combined) with the inclusion of the soluble fractions in the regression analyses. The use of the individual chemical components (NDF, ADF, cellulose, and lignin) was not effective in predicting the degradation characteristics. When the chemical components (cellulose, hemicellulose, lignin and total nitrogen) were combined in a multiple regression analysis, prediction was also poor. However, the results suggest that cellulose and lignin contribute significantly to the prediction. The inclusion of thewashing loss in the multiple regression analysis with the combined chemical components improved the prediction of ED. Calculated ED values from predictions correlated well with the experimentally determined ED. The coefficient of correlation between the observed and calculated ED values was highest for cattle (0.95) followed by sheep (0.93) and goats (0.77)

Derivation of a formula for adjusting the total serum calcium in Nigeria environment

The total calcium concentration, total protein, albumin and globulin were estimated for 302 patients that reported for serum calcium estimation at the clinical biochemistry laboratory of the Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria. Based on regression analysis, three formulae werederived for adjusting the total serum calcium concentration. It was observed that the total serum concentration correlated closely with albumin (a = 0.919 70) but poorly correlated with total protein (a =-0.25 960); where a = correlation coefficient. Adjusted calcium = total calcium – (0.91972 x albumin) +3.70429 was obtaine

Prevalence of HIV positive blood donors among screened volunteers who satisfied the criteria for blood donation in a semi-urban Nigeria population

Two thousand five hundred and thirty two (2,532) males, aged 25 – 50 years potential blood donors were randomly selected from the total number of volunteer blood donors who satisfied the initial screening criteria for donating blood, and were screened for HIV using Immunocomb II (HIV 1 and 2 Bispot) and Recombigen HIV-1/HIV-2RTD. Results from the study reveal that this procedure of screening volunteer donors by initial criteria alone does not fully eliminate all HIV positive donors. The prevalence of HIV positive donors among the screened volunteers who satisfied the criteria for blood donation was 0.87%.Keywords: Prevalence, blood donor, hiv positive and semi urban populatio

Prevalence of spontaneous bacterial peritonitis in liver cirrhosis with ascites

Introduction: Spontaneous bacterial peritonitis (SBP) is a common bacterial infection in patients with cirrhosis and ascites requiring prompt recognition and treatment. The aim of this study was to determine the prevalence, and characteristics of SBP among in-patients with cirrhosis and ascites seen at our facility.Methods: Thirty one patients with liver cirrhosis and ascites who were admitted into the Medical ward of the Ekiti State University Teaching Hospital (EKSUTH), Ado-Ekiti, Nigeria from August 2009 to July 2010 were retrospectively studied. All the patients had abdominal paracentesis done within 48 hours of admission under aseptic condition and the data obtained were analyzed. Results: The mean age of the studied population was 62±9 years (age range 43-78 years). Of the 21 that developed SPB, culture positive SBP was present in 66.7% (14/21) while CNNA was found in 33.3% (7/21). The prevalence of MNB was 26% (8/31) in this study. Of those with SBP, 93% had monomicrobial infection with aerobic Gram negative bacilli being responsible in 66.7% of the cases with E.coli (70%) being the predominant organism followed by Klebsiella species. Gram positive organisms accounted for 33.3% with Streptococcal species (60%) being the predominant organism followed by Staphylococcus aureus (40%). Patients with SBP had significantly lower platelet count when compared with those without SBP, p < 0.05. Also, international normalization ratio (INR) was significantly higher in those patients with SBP compared with those without SBP, p < 0.05. The poor prognostic indicators found in this study were; low ascitic protein, hepatic encephalopathy, coagulopathy, renal dysfunction (creatinine >2mg/dl) and leukocytosis (p < 0.05). Conclusion: It is therefore imperative to do diagnostic abdominal paracentesis for cell count and culture in any patient with onset of ascites or cirrhotic patients with ascites and suggestive symptoms compatible or suggestive of SBP.Key words: Spontaneous bacterial peritonitis, abdominal paracentesis, liver cirrhosis, ascite

Comparative effects of Apron plus 50DS and soil amendment on the growth, yield and food components of soybean

Comparative effects of Apron plus 50DS (a systemic fungicide) and soil amendment (poultry droppings) on growth yield and food components of soybean (TGX 1485-1D) were studied. Soybean seeds treated withrecommended dose of Apron plus 50DS had lower percentage germination when compared to the other treatments while the untreated seeds (control) had the highest percentage germination. Seeds treated with Apron plus and planted on amended soil gave the best performance in terms of development and yield. Biochemical analysis of harvested seeds showed an increase in protein content of seeds treated with recommended dose of Apron plus, planted on amended soil. Seeds treated with less than the recommended dose had the highest percentage carbohydrate content, while untreated seeds planted on amended soil had the lowest carbohydrate content. The control experiment had the highest percentage crude fibre while the lowestwas recorded for seeds treated with recommended dose of Apron plus, planted on amended soil. However, there is no significant difference among the treatments in percentage ash and moisture content

Chlamydial Infection, Plasma Peroxidation and Obesity in Tubal Infertility

Background: Genital tract infections and obesity are both sources of oxidative stress. Alterations in immune and antioxidant parameters may arise from this or from an indeterminate autoimmune mechanism.Objective: This study aimed to investigate the association of Chlamydial infection, obesity and oxidative response with tubal infertility in Nigerian women.Methods: It was a case-control study of 40 women with tubal infertility and 32 fertile women, respectively, recruited from the Infertility and Family Planning Clinics respectively, of the University College Hospital, Ibadan, Nigeria. Anthropometric indices were measured in each subject and endocervical swabs were taken to screen for current genital tract infection.Antioxidant, hormonal and immunologic analysis were performed on serum.Results: None of the subjects had current genital tract infections. Chlamydia trachomatis IgG positivity was significantly higher in infertile than in fertile subjects [OR 4.33; 95%CI (0.078-0.681)]. No significant variations were observed in the anthropometric indices, antioxidant parameters and hormones between infertile and the fertile women. Bodymass index correlated positively with oxidative stress in infertile subjects. Waist and hip circumferences correlated negatively with oestradiol in women with tubal infertility.Conclusion: Chlamydial infection is associated with tubal factor infertility, however, obesity seems to increase oxidative stress and reduce fertility potential in women with tubal factor infertility.Key words: Tubal infertility, obesity, oxidative stress, Chlamydi

Evaluation of fallow and cover crops for nematode suppression in three agroecologies of south western Nigeria

A study was conducted in three agroecological zones of south-western Nigeria to evaluate the effect of siam weed (Chromolaena odorata) and mucuna (Mucuna utilis) cover/fallow crops on plant-parasitic nematode population. The natural bush regrowth was used as control. Plant-parasitic nematodes were identified and counted during the fallow periods. Eleven genera of nematodes were identified and three (Meloidogyne, Pratylenchus, and Helicotylenchus) species were predominant across the trial locations.Other important genera present were Scutellonema, Tylenchorhynchus and Rotylenchus species. Nematode population densities of pre-crop were lowest in Alagba soil (Rhodic lixisols) at Ikenne in the wet forest zone of Nigeria. The populations in Iwo soil (Rhodic haplustalf) at Ibadan, a dry forest zonewas lower than in the Temidire soil (Plinthic luvisol) at Ilora in the derived savannah of south-western Nigeria. There was significant suppression of nematode population densities under the different crops as the fallow period increased. The population reduction in the different locations depended on the nematode species and the cover crops. The natural bush re-growth had the least effect on the nematode suppression at the end of the fallow period. On the average, siam weed fallow reduced nematode population densities by 67-79%, mucuna by 64-72% and the natural bush by 30-49% across the trial locations. For effective nematode suppression with fallow cropping, proper determination of the principal nematode species predominant in an environment is essential

Identification of compounds with cytotoxic activity from the leaf of the Nigerian medicinal plant, Anacardium occidentale L. (Anacardiaceae)

Cancer is now the second-leading cause of mortality and morbidity, behind only heart disease, necessitating urgent development of (chemo)therapeutic interventions to stem the growing burden of cancer cases and cancer death. Plants represent a credible source of promising drug leads in this regard, with a long history of proven use in the indigenous treatment of cancer. This study therefore investigated Anacardium occidentale, one of the plants in a Nigerian Traditional Medicine formulation commonly used to manage cancerous diseases, for cytotoxic activity. Bioassay-guided fractionation, spectroscopy, Alamar blue fluorescence-based viability assay in cultured HeLa cells and microscopy were used. Four compounds, zoapatanolide A (1), agathisflavone (2), 1,2-bis(2,6-dimethoxy-4-methoxycarbonylphenyl)ethane (anacardicin, 3) and methyl gallate (4), were isolated, with the most potent being zoapatanolide A with an IC50 value of 36.2 ± 9.8 µM in the viability assay. To gain an insight into the likely molecular basis of their observed cytotoxic effects, Autodock Vina binding free energies of each of the isolated compounds with seven molecular targets implicated in cancer development (MAPK8, MAPK10, MAP3K12, MAPK3, MAPK1, MAPK7 and VEGF), were calculated. Pearson correlation coefficients were obtained with experimentally-determined IC50 in the Alamar blue viability assay. While these compounds were not as potent as a standard anticancer compound, doxorubicin, the results provide reasonable evidence that the plant species contains compounds with cytotoxic activity. This study provides some evidence of why this plant is used ethnobotanically in anticancer herbal formulations and justifies investigating Nigerian medicinal plants highlighted in recent ethnobotanical surveys

Effectiveness of an Oral Health Care Training Workshop for School Teachers: A Pilot Study

Background: School teachers play key roles in imparting appropriate and up-to-date knowledge to pupils and students. However, most teachers in developing countries like Nigeria have poor knowledge and motivation about oral health which may be due to inadequate training in the area of oral health. This might be one of the reasons for the poor oral hygiene among them and their students.Objectives: To evaluate the effectiveness of an oral health care training programme organized for teachers in Eruwa, Oyo state, Nigeria. Methods: An intervention study was conducted among 40 school teachers who attended a two day oral health training workshop at Eruwa, headquarters of Ibarapa East Local Government Area. Training methods included lectures and demonstrations on aetiology, clinical features, treatment and prevention of common oral diseases. Pre- and post- evaluation written tests were administered to the participants to assess the effectiveness of the training. The knowledge scores were rated as poor, fair and good knowledge scoring <50.0%, 50.0-60.0% and > 60.0% respectively. Frequencies, percentages and means of relevant variables were generated. Paired t-test was used to compare means at P<0.05.Result: The mean age of the teachers was 40.13 ±7.24 years. There were 16 (40.0%) males and 24 (60.0%) females. Twenty (50.0%) of the participants had poor pre-training knowledge as compared to 7 (17.5%) after the training. Thirteen (32.5%) had fair pre-training knowledge as compared to 17 (42.5%) post training. Only (2.5%) of the participants had good pre-training knowledge as compared to 18 (45.0%) post training. The mean scores of the pre- and post-evaluation tests were 31.70 ±11.31 and 48.20 ±11.16 respectively. There was a statistically significant difference between the mean scores of the pre and post evaluations P<0.05.Conclusions: The training workshop improved the knowledge of school teachers regarding oral health.Keywords: Effectiveness, Oral health training, Teachers

Avoidable birth injury complicated with limb gangrene: a reflection of an in-efficient health system

Gangrene of the lower extremities in neonates is a rare event except in traumatic cases. When risk factors for trauma to the limb of the newborn exist, it is important to prevent such injuries and when they are inevitable, appropriate management of injuries should further prevent complications such as limb gangrene. This report describes a newborn whose birth was poorly managed and thereafter sustained traumatic unilateral lower limb gangrene from the management of femoral fracture at a traditional bone setting. This report aims to highlightthe deficiencies in the Nigerian health system which permitted this unfortunate scenario.Keywords: Birth injury, Lower limb gangrene, neonatal femoral fracture, unorthodox bone setting practice