Equivalent random analysis of a buffered optical switch with general interarrival times

We propose an approximate analytic model of an optical switch with fibre delay lines and wavelength converters by employing Equivalent Random Theory. General arrival traffic is modelled by means of Gamma-distributed interarrival times. The analysis is formulated in terms of virtual traffic flows within the optical switch from which we derive expressions for burst blocking probability, fibre delay line occupancy and mean delay. Emphasis is on approximations that give good numerical efficiency so that the method can be useful for formulating dimensioning problems for large-scale networks. Numerical solution values from the proposed analysis method compare well with results from a discrete-event simulation of an optical burst switch

Analytic modelling and resource dimensioning of optical burst switched networks

The realisation of optical network architectures may hold the key to delivering the enormous bandwidth demands of next generation Internet applications and services. Optical Burst Switching (OBS) is a potentially cost-effective switching technique that can satisfy these demands by offering a high bit rate transport service that is bandwidth-efficient under dynamic Internet traffic loads. Although various aspects of OBS performance have been extensively investigated, there remains a need to systematically assess the cost/performance trade-offs involved in dimensioning OBS switch resources in a network. This goal is essential in enabling the future deployment of OBS but poses a significant challenge due to the complexity of obtaining tractable mathematical models applicable to OBS network optimisation. The overall aim of this thesis lies within this challenge. This thesis firstly develops a novel analytic performance model of an OBS node where burst contention is resolved by combined use of Tuneable Wavelength Converters (TWCs) and Fibre Delay Lines (FDLs) connected in an efficient share-per-node configuration. The model uses a two-moment traffic representation that gives a good trade-off between accuracy and complexity, and is suitable for extension to use in network modelling. The OBS node model is then used to derive an approximate analytic model of an OBS network of switches equipped with TWCs and FDLs, again maintaining a two-moment traffic model for each end-to-end traffic path in the network. This allows evaluation of link/route loss rates under different offered traffic characteristics, whereas most OBS network models assume only a single-moment traffic representation. In the last part of this thesis, resource dimensioning of OBS networks is performed by solving single and multi-objective optimisation problems based on the analytic network model. The optimisation objectives relate to equipment cost minimisation and throughput maximisation under end-to-end loss rate constraints. Due to non-convexity of the network performance constraint equations, a search heuristic approach has been taken using a constraint-handling genetic algorithm

From Facility to Application Sensor Data: Modular, Continuous and Holistic Monitoring with DCDB

Today's HPC installations are highly-complex systems, and their complexity will only increase as we move to exascale and beyond. At each layer, from facilities to systems, from runtimes to applications, a wide range of tuning decisions must be made in order to achieve efficient operation. This, however, requires systematic and continuous monitoring of system and user data. While many insular solutions exist, a system for holistic and facility-wide monitoring is still lacking in the current HPC ecosystem. In this paper we introduce DCDB, a comprehensive monitoring system capable of integrating data from all system levels. It is designed as a modular and highly-scalable framework based on a plugin infrastructure. All monitored data is aggregated at a distributed noSQL data store for analysis and cross-system correlation. We demonstrate the performance and scalability of DCDB, and describe two use cases in the area of energy management and characterization.Comment: Accepted at the The International Conference for High Performance Computing, Networking, Storage, and Analysis (SC) 201

The Mont-Blanc Project: First Phase Successfully Finished

Running from October 2011 to June 2015, the aim of the European project Mont-Blanc has been to develop an approach to Exascale computing based on embedded power-efficient technology. The main goals of the project were to i) build an HPC prototype using currently available energy-efficient embedded technology, ii) design a Next Generation system to overcome the limitations of the built prototype and iii) port a set of representative Exascale applications to the system. This article summarises the contributions from the Leibniz Supercomputing Centre (LRZ) and the Juelich Supercomputing Centre (JSC), Germany, to the Mont-Blanc project.Comment: 5 pages, 3 figure

The Protective Effect of a Unique Mix of Polyphenols and Micronutrients against Neurodegeneration Induced by an In Vitro Model of Parkinson’s Disease

: Parkinson's disease (PD) is second-most common disabling neurological disorder worldwide, and unfortunately, there is not yet a definitive way to prevent it. Polyphenols have been widely shown protective efficacy against various PD symptoms. However, data on their effect on physio-pathological mechanisms underlying this disease are still lacking. In the present work, we evaluated the activity of a mixture of polyphenols and micronutrients, named A5+, in the murine neuroblastoma cell line N1E115 treated with 6-Hydroxydopamine (6-OHDA), an established neurotoxic stimulus used to induce an in vitro PD model. We demonstrate that a pretreatment of these cells with A5+ causes significant reduction of inflammation, resulting in a decrease in pro-inflammatory cytokines (IFN-γ, IL-6, TNF-α, and CXCL1), a reduction in ROS production and activation of extracellular signal-regulated kinases (ERK)1/2, and a decrease in apoptotic mechanisms with the related increase in cell viability. Intriguingly, A5+ treatment promoted cellular differentiation into dopaminergic neurons, as evident by the enhancement in the expression of tyrosine hydroxylase, a well-established dopaminergic neuronal marker. Overall, these results demonstrate the synergic and innovative efficacy of A5+ mixture against PD cellular pathological processes, although further studies are needed to clarify the mechanisms underlying its beneficial effect

The Mont-Blanc prototype: an alternative approach for high-performance computing systems

High-performance computing (HPC) is recognized as one of the pillars for further advance of science, industry, medicine, and education. Current HPC systems are being developed to overcome emerging challenges in order to reach Exascale level of performance,which is expected by the year 2020. The much larger embedded and mobile market allows for rapid development of IP blocks, and provides more flexibility in designing an application-specific SoC, in turn giving possibility in balancing performance, energy-efficiency and cost. In the Mont-Blanc project, we advocate for HPC systems be built from such commodity IP blocks, currently used in embedded and mobile SoCs. As a first demonstrator of such approach, we present the Mont-Blanc prototype; the first HPC system built with commodity SoCs, memories, and NICs from the embedded and mobile domain, and off-the-shelf HPC networking, storage, cooling and integration solutions. We present the system’s architecture, and evaluation including both performance and energy efficiency. Further, we compare the system’s abilities against a production level supercomputer. At the end, we discuss parallel scalability, and estimate the maximum scalability point of this approach across a set of HPC applications.Postprint (published version

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

The European Border and Coast Guard: An Improvement on Frontex? A Provisional Assessment

La grave crisis migratoria que ha afectado a la Unión Europea y sus Estados miembros en los últimos años, ha puesto en evidencia las limitaciones que presentan las actuaciones de la Agencia FRONTEX para hacerle frente. Con la adopción del Reglamento 2016/1624, se crea la Guardia Europea de Fronteras y Costas, que sustituye a FRONTEX y da continuidad a todas sus operaciones. El presente estudio está dedicado al análisis de esta nueva Agencia de la Unión Europea, tomando siempre como referente las actuaciones llevadas a cabo por su antecesora, FRONTEX. Como aspectos positivos que presenta la normativa que regula la Guardia Europea de Fronteras y Costas, cabe destacar que se prevé la creación de un contingente de reacción rápida permanente; se contempla una mayor financiación; se le atribuye competencias para organizar operaciones de retorno y se establece un mecanismo interno de denuncias. No obstante, en una valoración provisional, estos desarrollos normativos no parecen suficientes para resolver todas las limitaciones que presentaba FRONTEX con el objetivo de ofrecer una respuesta efectiva a la grave crisis migratoria. En última instancia, siempre se debe de tener en cuenta que, en gran medida, los Estados miembros siguen siendo los principales responsables de gestionar sus fronteras externas.The serious migratory crisis that has affected the European Union and its Member States in recent years has highlighted the limitations of the FRONTEX Agency's actions to tackle it. The European Border and Coast Guard was established by Regulation 2016/1624; it replaces FRONTEX and gives continuity to all its operations. The present study is dedicated to the analysis of this new Agency of the European Union, taking as a reference the actions carried out by its predecessor, FRONTEX. As a positive aspect of the regulations governing the European Border and Coast Guard, it should be noted that it is planned the creation of a permanent rapid reaction pool; increased funding is provided; powers to organize return operations are conferred and an internal complaints mechanism is established. However, in a provisional assessment, these normative developments do not seem sufficient to solve all the limitations presented by FRONTEX in order to provide an effective response to the serious migratory crisis. Ultimately, it must always be taken into account that Member States are still largely responsible for managing their external borders.Este trabajo se enmarca dentro de las actividades de investigación desarrolladas como miembro del proyecto de investigación “La Unión Europea frente a los Estados fracasados de su vecindario: retos y respuestas desde el Derecho internacional (II)” (DER2015-63498-C2-2-P [MINECO/FEDER])