75 research outputs found
Diesel Exhaust Activates & Primes Microglia: Air Pollution, Neuroinflammation, & Regulation of Dopaminergic Neurotoxicity
BACKGROUND:
Air pollution is linked to central nervous system disease, but the mechanisms responsible are poorly understood. OBJECTIVES:
Here, we sought to address the brain-region-specific effects of diesel exhaust (DE) and key cellular mechanisms underlying DE-induced microglia activation, neuroinflammation, and dopaminergic (DA) neurotoxicity. METHODS:
Rats were exposed to DE (2.0, 0.5, and 0 mg/m3) by inhalation over 4 weeks or as a single intratracheal administration of DE particles (DEP; 20 mg/kg). Primary neuron-glia cultures and the HAPI (highly aggressively proliferating immortalized) microglial cell line were used to explore cellular mechanisms. RESULTS:
Rats exposed to DE by inhalation demonstrated elevated levels of whole-brain IL-6 (interleukin-6) protein, nitrated proteins, and IBA-1 (ionized calcium-binding adaptor molecule 1) protein (microglial marker), indicating generalized neuroinflammation. Analysis by brain region revealed that DE increased TNFα (tumor necrosis factor-α), IL-1β, IL-6, MIP-1α (macrophage inflammatory protein-1α) RAGE (receptor for advanced glycation end products), fractalkine, and the IBA-1 microglial marker in most regions tested, with the midbrain showing the greatest DE response. Intratracheal administration of DEP increased microglial IBA-1 staining in the substantia nigra and elevated both serum and whole-brain TNFα at 6 hr posttreatment. Although DEP alone failed to cause the production of cytokines and chemokines, DEP (5 μg/mL) pretreatment followed by lipopolysaccharide (2.5 ng/mL) in vitro synergistically amplified nitric oxide production, TNFα release, and DA neurotoxicity. Pretreatment with fractalkine (50 pg/mL) in vitro ameliorated DEP (50 μg/mL)-induced microglial hydrogen peroxide production and DA neurotoxicity. CONCLUSIONS:
Together, these findings reveal complex, interacting mechanisms responsible for how air pollution may cause neuroinflammation and DA neurotoxicity
Chlorpyrifos Oxon Primes Microglia: Enhanced LPS-Induced TNFα Production
poster abstractMicroglia, the resident innate immune cells of the brain, respond to various environmental stimuli, including factors from surrounding tissue and from systemic inputs. These stimuli impact microglial function in both health and disease. Increasing evidence implicates microglia and neuroinflammation in Gulf War illness (GWI) pathology. Gulf War illness is an untreatable chronic multi symptomatic disorder that affects about 30% of Gulf War veterans. It has been proposed that “multiple hits” from exposure to various environmental neurotoxicants such as Chlorpyrifos (CPF), an organophosphate pesticide,
combined with low inflammation may initiate exaggerated and persistent central nervous system (CNS) pathology to drive GWI. CPF oxon, an active metabolite of CPF, is associated with deleterious CNS effects, but the role of microglia behind this phenomenon is not fully understood.To investigate the effects of CPF oxon on microglia, we assessed microglial ROS, pro-inflammatory cytokine factors, and NF-κB p50 DNA binding activity in the presence of CPF oxon. HAPI microglia cells were treated with CPF oxon (1μM-1nM), which resulted in a dose dependent increase in H2O2 production at 3 hours and elevated superoxide at 30 minutes. CPF oxon failed to initiate TNFα and nitric oxide from microglia cultures. However, CPF oxon significantly decreased NF-κB p50 binding to DNA in microglia, a key redox signaling mechanism linked to microglial priming. Consistent with this premise, pre-treatment with CPF oxon (0.5μM) amplified LPSinduced TNFα production in microglia and neuron-glia cultures. Moreover, when CPF oxon and LPS challenged cells were pre-treated with DPI, a NOX2 inhibitor, we found a significant reduction in TNFα response when compared to non-treated cells, supporting that NOX2 may regulate CPF oxon priming in microglia. These data suggest that CPF oxon may induce ROS production in microglia to reprogram these cells to become more sensitive to pro-inflammatory stimuli (priming)
Impact on Diet Quality and Resilience in Urban Community Dwelling Obese Women with a Nutrition and Physical Activity Intervention
Objective: To examine the effect of a Tai Chi, resistance training, and behaviorally-based diet education intervention on dietary quality as well as resilience and physical resilience in obese older women. Design: Community health outreach with a quasi-experimental design. Setting: An urban senior center in Rhode Island. Participants: Thirty-three women, 85% were minorities, with mean age of 65±8.2 years and BMI of 37.3±4.6 kg/m2, were enrolled in the study at baseline however only 17 women in the intervention (EXD) group and 9 women in the wait-list control (CON) group completed the study. Measurement: Dietary quality and nutrition risk were measured using the Dietary Screening Tool (DST), resilience was measured by the Resilience Scale, and physical resilience was examined using the Physical Resilience Scale. Intervention: Participants in the EXD group engaged in 12 weeks of Tai Chi, resistance training, and behaviorally-based diet education. The diet education was based off of the modified Dietary Approaches to Stop Hypertension (DASH) diet and led by a Registered Dietitian. Results: There was no change in dietary quality by group or time. However the EXD group had significantly higher dietary quality compared to the control group (p=0.025) at post intervention, although there was no difference in nutrition risk category. There was no change seen in overall resilience, however the EXD group improved physical resilience (p=0.048). Conclusion: A community health outreach that involved Tai Chi, resistance training, and behaviorally-based diet education may promote higher dietary quality as well as improve physical resilience in obese older women
Diesel Exhaust Activates and Primes Microglia: Air Pollution, Neuroinflammation, and Regulation of Dopaminergic Neurotoxicity
Background: Air pollution is linked to central nervous system disease, but the mechanisms responsible are poorly understood
Measured energy content of frequently purchased restaurant meals : multi-country cross sectional study
Funding: This work was supported in part by the US Department of Agriculture under agreement no. 58-1950-4-003 with Tufts University and a Tufts University Provost award to SBR. The study had additional funding in Brazil from FAPESP grants 2013/18520-0 and 2013/14489-1 to VS; in China from the National Science Foundation of China grant No 91431102 to JRS and International Partnership Program of Chinese Academy of Sciences grant No GJHZ1660 to JRS; in Finland from internal funding by the University of Eastern Finland to JP; in Ghana from the University of Georgia Global Research Collaborative Grant Program to AKA. The views expressed are those of the authors. The sponsors had no role in the design, undertaking, or reporting of the study.Peer reviewedPublisher PD
Effect of an Exercise and Dietary Intervention on Cognitive Function in Obese Older Women
LPS-preconditioned mesenchymal stromal cells modify macrophage polarization for resolution of chronic inflammation via exosome-shuttled let-7b
How the redox state regulates immunity
Oxidative stress is defined as an imbalance beween the levels of reactive oxygen species (ROS) and antioxidant defences. The view of oxidative stress as a cause of cell damage has evolved over the past few decades to a much more nuanced view of the role of oxidative changes in cell physiology. This is no more evident than in the field of immunity, where oxidative changes are now known to regulate many aspects of the immune response, and inflammatory pathways in particular. Our understanding of redox regulation of immunity now encompasses not only increases in reactive oxygen and nitrogen species, but also changes in the activities of oxidoreductase enzymes. These enzymes are important regulators of immune pathways both via changes in their redox activity, but also via other more recently identified cytokine-like functions. The emerging picture of redox regulation of immune pathways is one of increasing complexity and while therapeutic targeting of the redox environment to treat inflammatory disease is a possibility, any such strategy is likely to be more nuanced than simply inhibiting ROS production
Microglia processes associate with diffusely injured axons following mild traumatic brain injury in the micro pig
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