A Case Report of Malignant Mullerian Mixed Tumor of the Uterus

We experienced a case of malignant Mullerian mixed tumor of the uterus of a 58-year-old female. We performed an immunohistochemical study in order to analyze the expression of various antigens in different elements of this tumor. Keratin, carcinoembryonic antigen (CEA), and epithelial membrane antigen (EMA) showed strong positive results in epithelial component, while EMA also showed positive results in some areas of sarcomatous elements. On the other hand, vimentin and S-100 gave strong positive result in non-epithelial component, but also was positive to some extent in epithelial component in this tumor. Immunohistochemical method was useful to differentiate the different components of this tumor. However, EMA, Vimentin and S-100 showed some unusual results. This discrepancy may indicate a change in the nature of tumor cells due to `microenvironmental\u27 factors such as hormones and vita - mins. Therefore, careful evaluation is necessary to interpret the immunohistochemical results in surgical pathology

An Autopsy Case of Pleomorphic Leiomyosarcoma arising from the Uterus

A 49-year-old female patient was referred to this hospital due to uterine tumor. Needle biopsy of the uterine body was done after admission which suggested MFH (malignant fibrous histiocytoma) or poorly differentiated adenocarcinoma. The patient died after 11 days of admission before doing any surgical treatment for the tumor. Autopsy disclosed an adult head-sized pelvic tumor mass with multiple metastasis in lung, peritoneum and intestine. We confirmed the diagnosis of the present case as pleomorphic leiomyosarcoma by immunohistochemical study and electron microscopy in addition to routine light microscopy. The DNA distribution pattern of the tumor was determined by cytofluorometry and it was aneuploidy. The case report is presented with histological, immunohistochemical, electron microscopical, and cytofluorometrical study herein

HLA Genes and Haplotypes in Ryukyuans Suggest Recent Gene Flow to the Okinawa Islands

Polymorphism of HLA genes was investigated in a population sample of Ryukyuans living on the main island of Okinawa (n — 197), in the southwestern islands of Japan. Serological typing was applied to class I loci (HLA-A, -B, and -C) and to HLA-DRB1; nucleotide sequence-level typing was performed using PCR microtiter plate hybridization and PCR single-strand conformation polymorphism methods. Ryukyuans showed a higher frequency of DRB1*0405 and lower frequencies of DRB1*1502 and DRB1*1302 compared with Hondo Japanese living on main islands. Principal components and phylogenetic analyses of 12 East Asian populations, including Ryukyuans, were performed based on the allele frequencies of HLA-A, -B, and -DRB1. In the principal components analysis 3 Japanese populations (Ryukyuans, Hondo Japanese, and Ainu) formed a cluster and showed the highest affinity to 2 Korean populations. In the phylogenetic tree Ryukyuans and Ainu were neighbors, but the genetic distance between them was larger than the distances between Ryukyuans and Hondo Japanese and between Ryukyuans and Korean populations. The geographic cline of the predominant haplotype in Ryukyuans, A*24-B*54-DRB1*0405, suggests that an ancestral population possessing A*24-B*54-DRB1*0405 moved into the Okinawa Islands after the divergence of Ryukyuans from the Ainu. Such a recent gene flow, probably from South China to the Okinawa Islands, is considered the major cause of difference in genetic characteristics between Ryukyuans and the Ainu