The evolution of density currents and nepheloid bottom layers in the Ross Sea (Antarctica)

In this study we have analyzed the thermohaline, light transmission and particulate matter data, obtained in the western sector of the Ross Sea during the X Italian Expedition, for the purpose of investigating the evolution of the High Salinity Shelf Water in this area. In particular CTD data were used to estimate the baroclinic velocity field. Light transmission and total particulate matter data (from Niskin bottles mounted on a Carousel water sampler) were used to analyze the nepheloid layers and the evolution of the suspended sediments. This basin is characterized by a northward flow of very dense High Salinity Shelf Water (θ ∼ −1.95°C, S ∼ 34.90), much colder than the incoming Circumpolar Deep Water (θ ∼ 1.20°C, S ∼ 34.70). We obtained a scenario in which the High Salinity Shelf Water interacts with the Circumpolar Deep Water along the Antarctic Slope Front, and deviates from its geostrophic equilibrium. Interestingly, this cold dense water mixes with Circumpolar Deep Water at the shelf break and flows downward until it seems to disappear. Below this cold flow, a thin turbulent current has been observed, again moving northward with a high velocity ∼ 0.2–1.0 m s−1. This thin flow also contains high concentration of suspended matter produced by the interaction of the dense water and the bottom sediments. The various elementary mechanisms ruling the dynamics of such down-flows, namely the effects of topographic irregularities, bottom friction, Ekman benthic boundary layers or the effect of the variability of the Antarctic Circumpolar Current, which can push offshore the dense water, are discussed in this paper

Entendendo as mutações: a abordagem molecular e subcelular é suficiente?

This paper analyzes the productions of first-year high school students from public high schools in Montevideo, Uruguay, in a didactic sequence for the teaching of "mutations". Before and after this sequence, a semi-open questionnaire was applied, whose answers were compared. The didactic sequence incorporated analogies that were clearly favorable for understanding the genetic code and the mechanisms involved in the production of mutations. The responses to the questionnaire illustrate that, despite the above, "traces" of the general culture persist that hinder the assessment of possible implications of mutations at the level of organisms and species. The assignment of negative connotations to the term "mutation" predominates, as well as to structural changes, in particular deformations/malformations, and therefore associated with phenotypic changes. Both neutral effects and functional changes have a much lower association, making it difficult to associate this probability with humans without visible deformations. It follows that the understanding of the mechanisms at the molecular level must be complemented with contextualizing situations to link the various levels of biological organization and thus adequately dimension the concept of "mutation".Este trabajo analiza las producciones de estudiantes de primer año de bachillerato de liceos públicos de Montevideo, Uruguay, en una secuencia didáctica para la enseñanza de “mutaciones”. Antes y después de dicha secuencia se aplicó un cuestionario semiabierto, cuyas respuestas fueron comparadas. La secuencia didáctica incorporó analogías que claramente resultaron favorables para la comprensión del código genético y de los mecanismos implicados en la producción de mutaciones. Las respuestas al cuestionario ilustran que, a pesar de lo anterior, persisten “huellas” de la cultura general que obstaculizan la valoración de posibles implicancias de las mutaciones a nivel de organismos y de especies. Predomina la asignación de connotaciones negativas al término “mutación”, así como a cambios estructurales, en particular deformaciones/malformaciones, y por ende asociados a cambios fenotípicos. Tanto los efectos neutros como los cambios funcionales tienen una asociación bastante menor, resultando difícil asociar dicha probabilidad con humanos sin deformaciones visibles. Se desprende que la comprensión de los mecanismos a nivel molecular debe complementarse con situaciones contextualizadoras para vincular los diversos niveles de organización biológica y así dimensionar adecuadamente el concepto de “mutación”.   Este artigo analisa as produções de alunos do primeiro ano do ensino médio de escolas públicas de Montevidéu, Uruguai, em uma sequência didática para o ensino de "mutações". Antes e depois dessa sequência, foi aplicado um questionário semiaberto, cujas respostas foram comparadas. A sequência didática incorporava analogias claramente favoráveis ​​à compreensão do código genético e dos mecanismos envolvidos na produção de mutações. As respostas ao questionário ilustram que, apesar do exposto, persistem “vestígios” da cultura geral que dificultam a avaliação de possíveis implicações de mutações ao nível de organismos e espécies. Predomina a atribuição de conotações negativas ao termo "mutação", bem como a alterações estruturais, nomeadamente deformações/malformações, e por isso associadas a alterações fenotípicas. Tanto os efeitos neutros quanto as alterações funcionais têm uma associação muito menor, dificultando a associação dessa probabilidade com humanos sem deformações visíveis. Conclui-se que a compreensão dos mecanismos no nível molecular deve ser complementada com situações contextualizadoras para vincular os vários níveis de organização biológica e, assim, dimensionar adequadamente o conceito de "mutação"

Performance of two-dimensional Doppler echocardiography for the assessment of infarct size and left ventricular function in rats

Although echocardiography has been used in rats, few studies have determined its efficacy for estimating myocardial infarct size. Our objective was to estimate the myocardial infarct size, and to evaluate anatomic and functional variables of the left ventricle. Myocardial infarction was produced in 43 female Wistar rats by ligature of the left coronary artery. Echocardiography was performed 5 weeks later to measure left ventricular diameter and transverse area (mean of 3 transverse planes), infarct size (percentage of the arc with infarct on 3 transverse planes), systolic function by the change in fractional area, and diastolic function by mitral inflow parameters. The histologic measurement of myocardial infarction size was similar to the echocardiographic method. Myocardial infarct size ranged from 4.8 to 66.6% when determined by histology and from 5 to 69.8% when determined by echocardiography, with good correlation (r = 0.88; P < 0.05; Pearson correlation coefficient). Left ventricular diameter and mean diastolic transverse area correlated with myocardial infarct size by histology (r = 0.57 and r = 0.78; P < 0.0005). The fractional area change ranged from 28.5 ± 5.6 (large-size myocardial infarction) to 53.1 ± 1.5% (control) and correlated with myocardial infarct size by echocardiography (r = -0.87; P < 0.00001) and histology (r = -0.78; P < 00001). The E/A wave ratio of mitral inflow velocity for animals with large-size myocardial infarction (5.6 ± 2.7) was significantly higher than for all others (control: 1.9 ± 0.1; small-size myocardial infarction: 1.9 ± 0.4; moderate-size myocardial infarction: 2.8 ± 2.3). There was good agreement between echocardiographic and histologic estimates of myocardial infarct size in rats.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Disciplina de Fisiologia CardiovascularUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Disciplina de CardiologiaUNIFESP, EPM, Disciplina de Fisiologia CardiovascularUNIFESP, EPM, Disciplina de CardiologiaSciEL

O “Gostar de Matemática”: em busca de uma interpretação psicanalítica

Nos últimos anos, temos procurado investigar as razões pelas quais estudantes da graduação de áreas das ciências ditas exatas ou naturais optaram pelo curso que fazem. Quase sempre surge espontaneamente em suas falas a palavra gostar como principal resposta às nossas perguntas. Neste trabalho, propomos uma interpretação para o significante ‘gostar’ que aparece no enunciado “gostar de Matemática”, a partir de alguns conceitos da psicanálise de orientação lacaniana. Valemo-nos de entrevistas realizadas com alunos da graduação em Matemática, da Universidade Estadual de Londrina

Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits

Objective: To investigate the VEGF expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits. Materials and Methods: After partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. Groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. Sections of 5 mu m were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. To confirm the presence of VEGF in tissues, protein expression was determined by immunohistochemistry. Results: No death, urinary leakage or graft extrusion occurred in any group. All bladders showed a spherical shape. A progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p &lt; 0.001). VEGF expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p &lt; 0.001). Conclusion: The latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process

Immunosenescence and lymphomagenesis

One of the most important determinants of aging-related changes is a complex biological process emerged recently and called \u201cimmunosenescence\u201d. Immunosenescence refers to the inability of an aging immune system to produce an appropriate and effective response to challenge. This immune dysregulation may manifest as increased susceptibility to infection, cancer, autoimmune disease, and vaccine failure. At present, the relationship between immunosenescence and lymphoma in elderly patients is not defined in a satisfactory way. This review presents a brief overview of the interplay between aging, cancer and lymphoma, and the key topic of immunosenescence is addressed in the context of two main lymphoma groups, namely Non Hodgkin Lymphoma (NHL) and Hodgkin Lymphoma (HL). Epstein Barr Virus (EBV) plays a central role in the onset of neoplastic lymphoproliferation associated with immunological changes in aging, although the pathophysiology varies vastly among different disease entities. The interaction between immune dysfunction, immunosenescence and Epstein Barr Virus (EBV) infection appears to differ between NHL and HL, as well as between NHL subtypes

PRISM (Polarized Radiation Imaging and Spectroscopy Mission): A White Paper on the Ultimate Polarimetric Spectro-Imaging of the Microwave and Far-Infrared Sky

PRISM (Polarized Radiation Imaging and Spectroscopy Mission) was proposed to ESA in response to the Call for White Papers for the definition of the L2 and L3 Missions in the ESA Science Programme. PRISM would have two instruments: (1) an imager with a 3.5m mirror (cooled to 4K for high performance in the far-infrared---that is, in the Wien part of the CMB blackbody spectrum), and (2) an Fourier Transform Spectrometer (FTS) somewhat like the COBE FIRAS instrument but over three orders of magnitude more sensitive. Highlights of the new science (beyond the obvious target of B-modes from gravity waves generated during inflation) made possible by these two instruments working in tandem include: (1) the ultimate galaxy cluster survey gathering 10e6 clusters extending to large redshift and measuring their peculiar velocities and temperatures (through the kSZ effect and relativistic corrections to the classic y-distortion spectrum, respectively) (2) a detailed investigation into the nature of the cosmic infrared background (CIB) consisting of at present unresolved dusty high-z galaxies, where most of the star formation in the universe took place, (3) searching for distortions from the perfect CMB blackbody spectrum, which will probe a large number of otherwise inaccessible effects (e.g., energy release through decaying dark matter, the primordial power spectrum on very small scales where measurements today are impossible due to erasure from Silk damping and contamination from non-linear cascading of power from larger length scales). These are but a few of the highlights of the new science that will be made possible with PRISM.Comment: 20 pages Late

SARS-CoV-2 omicron (B.1.1.529)-related COVID-19 sequelae in vaccinated and unvaccinated patients with cancer: results from the OnCovid registry

Background COVID-19 sequelae can affect about 15% of patients with cancer who survive the acute phase of SARS-CoV-2 infection and can substantially impair their survival and continuity of oncological care. We aimed to investigate whether previous immunisation affects long-term sequelae in the context of evolving variants of concern of SARS-CoV-2. Methods OnCovid is an active registry that includes patients aged 18 years or older from 37 institutions across Belgium, France, Germany, Italy, Spain, and the UK with a laboratory-confirmed diagnosis of COVID-19 and a history of solid or haematological malignancy, either active or in remission, followed up from COVID-19 diagnosis until death. We evaluated the prevalence of COVID-19 sequelae in patients who survived COVID-19 and underwent a formal clinical reassessment, categorising infection according to the date of diagnosis as the omicron (B.1.1.529) phase from Dec 15, 2021, to Jan 31, 2022; the alpha (B.1.1.7)-delta (B.1.617.2) phase from Dec 1, 2020, to Dec 14, 2021; and the pre-vaccination phase from Feb 27 to Nov 30, 2020. The prevalence of overall COVID-19 sequelae was compared according to SARS-CoV-2 immunisation status and in relation to post-COVID-19 survival and resumption of systemic anticancer therapy. This study is registered with ClinicalTrials.gov, NCT04393974. Findings At the follow-up update on June 20, 2022, 1909 eligible patients, evaluated after a median of 39 days (IQR 24-68) from COVID-19 diagnosis, were included (964 [ 50 center dot 7%] of 1902 patients with sex data were female and 938 [49 center dot 3%] were male). Overall, 317 (16 center dot 6%; 95% CI 14 center dot 8-18 center dot 5) of 1909 patients had at least one sequela from COVID-19 at the first oncological reassessment. The prevalence of COVID-19 sequelae was highest in the prevaccination phase (191 [19 center dot 1%; 95% CI 16 center dot 4-22 center dot 0] of 1000 patients). The prevalence was similar in the alpha-delta phase (110 [16 center dot 8%; 13 center dot 8- 20 center dot 3] of 653 patients, p=0 center dot 24), but significantly lower in the omicron phase (16 [6 center dot 2%; 3 center dot 5-10 center dot 2] of 256 patients, p<0 center dot 0001). In the alpha- delta phase, 84 (18 center dot 3%; 95% CI 14 center dot 6-22 center dot 7) of 458 unvaccinated patients and three (9 center dot 4%; 1 center dot 9- 27 center dot 3) of 32 unvaccinated patients in the omicron phase had sequelae. Patients who received a booster and those who received two vaccine doses had a significantly lower prevalence of overall COVID-19 sequelae than unvaccinated or partially vaccinated patients (ten [7 center dot 4%; 95% CI 3 center dot 5-13 center dot 5] of 136 boosted patients, 18 [9 center dot 8%; 5 center dot 8-15 center dot 5] of 183 patients who had two vaccine doses vs 277 [ 18 center dot 5%; 16 center dot 5-20 center dot 9] of 1489 unvaccinated patients, p=0 center dot 0001), respiratory sequelae (six [4 center dot 4%; 1 center dot 6-9 center dot 6], 11 [6 center dot 0%; 3 center dot 0-10 center dot 7] vs 148 [9 center dot 9%; 8 center dot 4- 11 center dot 6], p= 0 center dot 030), and prolonged fatigue (three [2 center dot 2%; 0 center dot 1-6 center dot 4], ten [5 center dot 4%; 2 center dot 6-10 center dot 0] vs 115 [7 center dot 7%; 6 center dot 3-9 center dot 3], p=0 center dot 037)

A machine-learning based bio-psycho-social model for the prediction of non-obstructive and obstructive coronary artery disease

Background: Mechanisms of myocardial ischemia in obstructive and non-obstructive coronary artery disease (CAD), and the interplay between clinical, functional, biological and psycho-social features, are still far to be fully elucidated. Objectives: To develop a machine-learning (ML) model for the supervised prediction of obstructive versus non-obstructive CAD. Methods: From the EVA study, we analysed adults hospitalized for IHD undergoing conventional coronary angiography (CCA). Non-obstructive CAD was defined by a stenosis &lt; 50% in one or more vessels. Baseline clinical and psycho-socio-cultural characteristics were used for computing a Rockwood and Mitnitski frailty index, and a gender score according to GENESIS-PRAXY methodology. Serum concentration of inflammatory cytokines was measured with a multiplex flow cytometry assay. Through an XGBoost classifier combined with an explainable artificial intelligence tool (SHAP), we identified the most influential features in discriminating obstructive versus non-obstructive CAD. Results: Among the overall EVA cohort (n = 509), 311 individuals (mean age 67 ± 11&nbsp;years, 38% females; 67% obstructive CAD) with complete data were analysed. The ML-based model (83% accuracy and 87% precision) showed that while obstructive CAD was associated with higher frailty index, older age and a cytokine signature characterized by IL-1β, IL-12p70 and IL-33, non-obstructive CAD was associated with a higher gender score (i.e., social characteristics traditionally ascribed to women) and with a cytokine signature characterized by IL-18, IL-8, IL-23. Conclusions: Integrating clinical, biological, and psycho-social features, we have optimized a sex- and gender-unbiased model that discriminates obstructive and non-obstructive CAD. Further mechanistic studies will shed light on the biological plausibility of these associations. Clinical trial registration: NCT02737982