Lysogenic System of Non-Epidemic Cholera Vibrio El Tor, Resistant to Diagnostic Bacteriophage CTX-

Studied are non-epidemic strains of V. cholerae O1 biovar El Tor taken from the National collection of pathogenic bacteria lodged at the Russian Research Anti-Plague Institute Microbe, which were not sensible to diagnostic cholera bacteriophage El Tor ctx- at the time of isolation. Represented are the results of investigation regarding a cause of a phage-resistance of these cultures using specialized test-system consisting of indicator strains. Identified is the carriage of temperate phages among 88,0 % of the cultures examined

Prospective Sorption Matrices for Antitoxic Cholera Enterosorbent Constructing

Demonstrated is the possibility of constructing of oral anti-cholera preparations based on sorption matrices with immobilized specific ligand. Various enterosorbents (activated charcoal, polysorb, polyfepan, and chitosan) have been used as sorption matrices, cholera antitoxic immunoglobulins (ATIg) – as specific ligands. Toxin-neutralizing activity of the ATIg immobilized on polysorb has been observed in vivo in 1:64000 dilutions and that of ATIg immobilized on activated charcoal, polyfepan, and chitosan – in 1:32000. Their specific activity in vitro has been defined in dot-blot immunoassay in 1:10000 and 1:5000 dilutions, respectively. High toxin-neutralizing activity, as well as specific one has proved the suitability of all tested matrices for antitoxic cholera enterosorbents constructing. However, polysorb and chitosan are accepted to be the most prospective matrices in view of the studied characteristics and properties. To stabilize the properties of the obtained experimental prototype sorbents, AT/Ig-polysorb and AT/Ig-chitosan, applied has been the method of liophilisation with glycocol stabilizer. Dried samples retain their original biological properties

Differences of the 57Fe hyperfine parameters in both oxyhemoglobin and spleen from normal human and patient with primary myelofibrosis

Study of oxyhemoglobin in red blood cells and spleen tissues from normal human and patient with primary myelofibrosis was carried out using Mössbauer spectroscopy with a high velocity resolution. The 57Fe hyperfine parameters were evaluated and small variations in quadrupole splitting were revealed for both normal human and patient's oxyhemoglobin and both normal human and patient's spleen. © 2012 Springer Science+Business Media Dordrecht

Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C

New nutrient media based on baker yeast autolizate were used for the first time for manufacturing of diagnostic preparation of plague bacteriophage L-413C. Experimental media provide high concentration of phage particles at the stage of propagation, and good survivability in lyophilization. Media in which yeast autolizate was a nutrient protein basis appeared to be more effective than those in which it was a stimulating additive. Phage preparations preserved stability of properties during storage at 4-8 °С, and at a higher temperature in the test of accelerated aging. Introduction of yeast nutrient media in technology of plague diagnostic bacteriophage L-413C manufacturing opens good prospects for increasing of production efficiency and decreasing of cost value of the preparatio

Development and Main Stages of Introduction of the Preparation “Cholera O139 Diagnostic Fluorescent Immunoglobulins”

– for environmental objects. Application of the preparation for practical purposes was considered to be promising, and it was recommended for State registration as a product of medical application

Prospects for Application of Ultrafiltration Technology for the Scaled Preparation of Plague Microbe and Cholera Vibrio Major Antigens

Demonstrated is the possibility of application of ultrafiltration technologies in the process of cholera toxin and plague agent capsular antigen precipitation under production conditions. Application of ultrafiltration techniques permits of the reduction of losses at the stages of isolation and purification of antigen preparations; and concentration of raw material or semi-finished product provides for the reduction of labor inputs. Thus it leads to the increase in productivity and economical efficiency

Non-Waste Alternative Technologies in the Production of Heterologous Anti-Rabies Immunoglobulin

Presented is a comprehensive approach to utilization of the wastes that appear in the process of heterologous anti-rabies immunoglobulin production (packed red cells, fibrin, and alcohol-containing products). Specific immunoglobulin is extracted from the surface of red blood cells using desorption technique. Additional yields of immunoglobulin after exposure of erythrocytes to non-ionic detergent amount to 10-19 % of the output. Rich protein supplement feeding for horses-producers is obtained from spray-dried packed red cells. Solid nutritious substrate for microbiological media production is obtained from fibrin using enzymic hydrolysis method. The efficiency of the fibrin hydrolysate-based media is 1.5-2 times higher in comparison with that of the media based on the digest of meat and casein, as demonstrated by the results of Vibrio cholerae scaled cultivation. Furthermore, worked out is the technology of ethanol regeneration after the rivanol-ethanolic precipitation of gamma globulin, alcohol content by volume being (93±1) % after the regeneration. It is demonstrated that the regenerated alcohol can be used as a precipitator in the process of anti-rabies serum fractioning. All in all, the developed techniques make it possible to utilize the wastes of anti-rabies immunoglobulin production and provide for further use of derivatives while producing medical immunobiological preparations

Comparative Assessment of <i>Yersinia pestis</i> Strains on the Level of Plague Microbe Capsular Antigen Production

Yersinia pestis recombinant strain KM277 (EV11MpFSK-3) Kmr is a potential producer-strain of capsular antigen (F1), since its productive properties and immunochemical activity of F1-antigen, synthesized by it, are higher than in Y. pestis EV and its derivatives. The apparent advantage of the Y. pestis KM277 strain is the ability to synthesize F1 antigen at 28 °C, and the absence of its own plague-microbe plasmids in cells. Worked out is the scheme of scaled cultivation of the recombinant strain and obtainment of capsular antigen preparation