The Assembly Pathway of the 19S Regulatory Particle of the Yeast 26S Proteasome([Image: see text])

The 26S proteasome consists of the 20S proteasome (core particle) and the 19S regulatory particle made of the base and lid substructures, and it is mainly localized in the nucleus in yeast. To examine how and where this huge enzyme complex is assembled, we performed biochemical and microscopic characterization of proteasomes produced in two lid mutants, rpn5-1 and rpn7-3, and a base mutant ΔN rpn2, of the yeast Saccharomyces cerevisiae. We found that, although lid formation was abolished in rpn5-1 mutant cells at the restrictive temperature, an apparently intact base was produced and localized in the nucleus. In contrast, in ΔN rpn2 cells, a free lid was formed and localized in the nucleus even at the restrictive temperature. These results indicate that the modules of the 26S proteasome, namely, the core particle, base, and lid, can be formed and imported into the nucleus independently of each other. Based on these observations, we propose a model for the assembly process of the yeast 26S proteasome

Search for a Higgs boson in the diphoton final state in pp collisions at sqrt[s]=1.96TeV.

A search for a narrow Higgs boson resonance in the diphoton mass spectrum is presented based on data corresponding to 7.0fb{-1} of integrated luminosity from pp collisions at sqrt[s]=1.96TeV collected by the CDF experiment. No evidence of such a resonance is observed, and upper limits are set on the cross section times branching ratio of the resonant state as a function of Higgs boson mass. The limits are interpreted in the context of the standard model and one fermiophobic benchmark model where the data exclude fermiophobic Higgs bosons with masses below 114GeV/c{2} at a 95% Bayesian credibility level

Search for the Higgs boson in the all-hadronic final state using the CDF II detector

We report on a search for the production of the Higgs boson decaying to two bottom quarks accompanied by two additional quarks. The data sample used corresponds to an integrated luminosity of approximately 4 fb(-1) of p (p) over bar collisions at root s = 1.96 TeV recorded by the CDF II experiment. This search includes twice the integrated luminosity of the previous published result, uses analysis techniques to distinguish jets originating from light flavor quarks and those from gluon radiation, and adds sensitivity to a Higgs boson produced by vector boson fusion. We find no evidence of the Higgs boson and place limits on the Higgs boson production cross section for Higgs boson masses between 100 GeV/c(2) and 150 GeV/c(2) at the 95% confidence level. For a Higgs boson mass of 120 GeV/c(2), the observed (expected) limit is 10.5 (20.0) times the predicted standard model cross section